Development of an SPF Macaca nemestrina Breeding Colony

SPF 猕猴饲养群的开发

• 批准号: 9109080
• 负责人: ROBERT J. ADAMS
• 金额: $ 93.46万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-21 至 2017-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9109080
• 关键词: Alleles; Animal Model; Animals; Area; Breeding; Cercopithecine Herpesvirus 1; Characteristics; Communities; Data; Development; Disease; Disease Management; Disease Outcome; Enterocolitis; Family suidae; Female; Frequencies; Funding; Genetic; Genetic Markers; Genome; Genotype; Grant; Immunogenetics; Institutes; Institution; Left; Letters; MHC Class I Genes; Macaca; Macaca nemestrina; Maps; Request for Proposals; Research; Research Personnel; Resources; SIV; Sales; Serologic tests; Serological; Simian Retroviruses; Single Nucleotide Polymorphism; Tail; Testing; Time; United States National Institutes of Health; Universities; Viral; Virus; Work; animal resource; base; genetic pedigree; germ free condition; health data; human disease; knowledge base; male; member

This resubmission of our animal resource grant renewal proposal requests five years of funding to continue support for a Specific Pathogen Free (SPF) colony of pig-tailed macaques (Macaca nemestrina) at the Johns Hopkins University that was first funded on September 21, 2006. It has the following resource component and research component aims: A. Resource Component Related Aims: (1) To expand the size of the current SPF breeding colony to increase the numbers of females for breeding and males available for sale to NIH funded investigators both within Johns Hopkins University and at other research institutions. (2) To maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV). At present all of the current members of the colony have repeatedly tested negative for these agents. (3) To maintain pedigree and health data on all animals. This data is available on all of the animals in the colony and will be expanded as the colony grows. B. Research Component Aims: To expand the genetic data available for this species to a) increase the research utility of the species and b) assist in disease management of the colony. This will be done through three research aims. (1) To characterize pig-tailed macaque immunogenetics in detail via MHC class I genotyping. (2) To characterize pig-tailed macaque genetics by single nucleotide polymorphism (SNP) genetic marker mapping throughout the pig-tailed macaque genome. (3) To determine whether either MHC class I allele expression or SNPs are associated with a) SIV-related disease outcomes and b) naturally occurring enterocolitis in pig-tailed macaques.

我们重新提交的动物资源拨款续订提案要求提供五年的资金，以继续支持约翰·霍普金斯大学的无特定病原体 (SPF) 猪尾猕猴 (Macaca nemestrina) 群体，该群体于 2006 年 9 月 21 日首次获得资助。它具有以下资源组成部分和研究组成目标： A. 资源组件相关目标： (1) 扩大当前 SPF 繁殖群体的规模，以增加用于繁殖的雌性和可供出售给 NIH 资助的约翰·霍普金斯大学和其他研究机构的研究人员的雄性数量。 (2) 通过定期血清学和 PCR 检测 4 种病毒来维持菌落的 SPF 状态：猴疱疹病毒 1（B 病毒）、猿免疫缺陷病毒 (SIV)、猿逆转录病毒 (SRV) 和猿 T 淋巴细胞病毒 (STLV) ）。目前，该群体的所有现有成员均多次检测出这些物质呈阴性。 (3) 保存所有动物的谱系和健康数据。该数据适用于群体中的所有动物，并将随着群体的发展而扩展。 B. 研究部分目标：扩大该物种可用的遗传数据，以 a) 增加该物种的研究效用，b) 协助群体的疾病管理。这将通过三个研究目标来完成。 (1) 通过 MHC I 类基因分型详细表征猪尾猕猴免疫遗传学。 (2) 通过整个猪尾猕猴基因组的单核苷酸多态性（SNP）遗传标记图谱来表征猪尾猕猴遗传学。 (3) 确定 MHC I 类等位基因表达或 SNP 是否与 a) SIV 相关疾病结果和 b) 猪尾猕猴中自然发生的小肠结肠炎相关。