All-Optical Methods for Studying Sequential Motor Behaviors

研究顺序运动行为的全光学方法

• 批准号: 9203398
• 负责人: TODD F ROBERTS
• 金额: $ 11.24万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-22 至 2018-02-21
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9203398
• 关键词: BRAIN initiative; Behavior; Behavioral; Birds; Brain; Brain region; Calcium; Calcium Signaling; Cells; Collaborations; Complex; Electrophysiology (science); Exhibits; Generations; Gestures; Goals; Image; Laboratories; Language; Lasers; Learning; Light; Lighting; London; Maps; Methods; Microscope; Microscopy; Motor; Neurons; Neurosciences; Optical Methods; Optics; Pattern; Performance; Physiologic pulse; Physiological; Physiology; Play; Population; Production; Property; Protocols documentation; Research; Resolution; Scanning; Series; Songbirds; Stereotyping; Study models; Synapses; System; Techniques; Testing; Training; Universities; Visit; Whole-Cell Recordings; bird song; calcium indicator; college; design; in vivo; insight; learned behavior; liquid crystal; neuronal patterning; novel; optogenetics; relating to nervous system; research study; two-photon; vocal learning

PROJECT SUMMARY The execution of learned sequential motor behaviors, like those involved in playing a well learned tune on the piano, are thought to be supported by precise sequences of neuronal activity in the brain. However, probing the ties between sequential neuronal activity, neuronal connectivity and behavior is challenging without methods for simultaneously observing and controlling neuronal activity with spatial and temporal precision. We propose to apply all-optical physiological methods, combining concurrent optogenetic manipulation, and population calcium imaging, to map the functional organization of circuits involved in a well-studied sequential motor behavior. This research will involve dissemination of cutting-edge optical methods central to the BRAIN Initiative and facilitate a better understanding of how patterns of neuronal activity underlying precise complex behaviors are generated in the brain.

项目概要 执行习得的顺序运动行为，例如演奏一首习得的曲子 在钢琴上，被认为是由大脑中精确的神经元活动序列支持的。然而， 探究连续的神经元活动、神经元连接和行为之间的联系是具有挑战性的，如果没有 以空间和时间精度同时观察和控制神经元活动的方法。我们 提出应用全光学生理方法，结合并发光遗传学操作，以及 群体钙成像，绘制经过充分研究的序列所涉及的电路的功能组织图 运动行为。这项研究将涉及传播大脑核心的尖端光学方法 主动并促进更好地理解精确复杂的神经元活动模式 行为是在大脑中产生的。