Rocky Mountain Neurological Disorders Core

落基山神经疾病核心

基本信息

批准号：
8427301
负责人：
Angeles Badell Ribera
金额：
$ 11.72万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-09-15 至
项目状态：
未结题

项目摘要

Zebrafish Core C3.1 Rationale. Core C maintains wild type, transgenic and mutant zebrafish strains for RMNDC investigators. The unique developmental and optical imaging properties of the zebrafish embryo makes it an ideal model to address questions at the heart of the research programs RMNDC investigators. UCD NINDS researchers are internationally recognized and expert in the fields of neurophysiology, neurodevelopment and optical imaging. During the previous award period. Core C removed technical barriers for RMNDC investigators who were interested in using the model system (e.g., Betz, Caldwell, Clouthier, Niswander, Restrepo, Taylor, Williams) but did not have experience or means for maintaining lines. We propose three Specific Aims for Core C that will allow RMNDC members to take advantage of the zebrafish system for neurodevelopmental and behavioral studies. In addition to the 13 current Core C users, the facility will allow access to the zebrafish model to investigators who have not considered its use because of the pragmatic limitations associated with maintaining and breeding zebrafish. In particular, there has been growing interest in complementing rodent studies (e.g.. Core B) with ones done in zebrafish (e.g., Appel, Artinger, Barlow, Clouthier, Macklin, Niswander, Restrepo, Williams), and vice versa. The core will also be a resource for the larger scientific community because transgenic lines will be described on the RMNDC website and made available to the Zebrafish International Resource Center (ZIRC) by the time of the first publication.

斑马鱼核心 C3.1 基本原理。 Core C 为 RMNDC 研究人员维护野生型、转基因和突变斑马鱼品系。斑马鱼胚胎独特的发育和光学成像特性使其成为解决 RMNDC 研究人员研究项目核心问题的理想模型。 UCD NINDS 研究人员是神经生理学、神经发育和光学成像领域国际公认的专家。上一奖励期间。核心 C 为有兴趣使用模型系统的 RMNDC 调查人员消除了技术障碍（例如 Betz、Caldwell、Clouthier、Niswander、Restrepo、Taylor、Williams）但没有维护线路的经验或手段。我们为核心 C 提出了三个具体目标，使 RMNDC 成员能够利用斑马鱼系统进行神经发育和行为研究。除了当前的 13 个 Core C 用户之外，该设施还将允许那些由于与维持和繁殖斑马鱼相关的实用限制而未考虑使用斑马鱼模型的研究人员访问斑马鱼模型。特别是，已经有人们越来越有兴趣用斑马鱼研究（例如 Appel、Artinger、Barlow、Clouthier、Macklin、Niswander、Restrepo、Williams）来补充啮齿动物研究（例如 Core B），反之亦然。该核心也将成为更大科学界的资源，因为转基因品系将在 RMNDC 网站上进行描述，并在首次出版时提供给斑马鱼国际资源中心 (ZIRC)。