CSHL Advanced Techniques In Molecular Neuroscience Course

CSHL 分子神经科学高级技术课程

• 批准号: 8934990
• 负责人: DAVID J. STEWART
• 金额: $ 10.57万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-06 至 2020-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8934990
• 关键词: Affinity Chromatography; Animal Viruses; Applied Research; Area; Attention; Basic Science; Biological Assay; Chromatin; Chromatin Structure; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; Disease; Educational Curriculum; Educational process of instructing; Electrophysiology (science); Electroporation; Ensure; Environment; Exercise; Faculty; Funding; Future; Gene Transfer; Genes; Home environment; Image; Individual; Infection; Institution; Laboratories; Left; Mainstreaming; Mammalian Cell; Mental Health; Messenger RNA; Methodology; Methods; Molecular; Molecular Neurobiology; Neurobiology; Neuroglia; Neurons; Neurosciences; Neurosciences Research; New York; Participant; Pharmacology; Physicians; Postdoctoral Fellow; Process; Publications; RNA Interference; Reporting; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Ribosomes; Role; Scientist; Series; Specific qualifier value; Students; Techniques; Time; Training; Training Programs; Transfection; Transgenic Organisms; Translating; United States; Update; Work; arm; base; career; design; experience; forging; gene delivery system; genome editing; graduate student; in vivo; instructor; interest; laboratory manuals; lecturer; medical specialties; member; nervous system disorder; programs; public health relevance; relating to nervous system; vector

 DESCRIPTION (provided by applicant): CSHL Laboratory Course in Advanced Techniques in Molecular Neuroscience The proposed Cold Spring Harbor Laboratory (CSHL) course Advanced Techniques in Molecular Neuroscience (ATMN), to be held annually in July 2015 - 2019, is part of the CSHL's postgraduate training program in neurobiology. ATMN is a short, intensive 2.5 week hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of neurobiological research. Experimental modules in the course will include an introduction to the design and use of animal virus vectors in neuroscience research; the use of CRISPR genome editing and RNAi approaches for regulating the expression of specific genes in neurons and glia; practical exercises in gene delivery systems including mammalian cell infection and transfection and electroporation techniques for targeted gene transfer in vivo; an introduction to overall strategies, use and design of BAC transgenic vectors; real time RT-PCR analyses; assays of chromatin and chromatin structure in neurons, and mRNA isolation from specified neural subtypes by translating ribosome affinity purification. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The ATMN course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.

 描述（由申请人提供）：CSHL 分子神经科学高级技术实验室课程 拟议的冷泉港实验室 (CSHL) 分子神经科学高级技术 (ATMN) 课程将于 2015 年 7 月至 2019 年每年举办一次，是 CSHL 课程的一部分ATMN 神经生物学研究生培训项目是一门为期 2.5 周的短期强化实践课程，帮助学生做好直接进入研究的准备。课程中的实验模块将介绍先进和/或专门的分子技术在神经科学研究中的设计和使用；使用 CRISPR 基因组编辑和 RNAi 方法来调节神经生物学。神经元和神经胶质细胞中特定基因的表达；基因传递系统的实践练习，包括哺乳动物细胞感染和转染以及体内靶向基因转移的电穿孔技术；BAC 转基因载体的总体策略、使用和设计的介绍；神经元中染色质和染色质结构的测定，以及通过翻译核糖体亲和纯化从特定神经亚型中分离 mRNA。本课程邀请在其可能提供的更广泛的概念进展的背景下教授实验技术。由于培训时间较短，培训生是由课程教师从大量申请者中挑选出来的，其中包括研究生、高级研究人员和研究医师。 ATMN 课程还为成熟的科学家提供了一个不寻常的机会，让他们能够将这些先进技术应用到实践中。课程中教授的方法和概念通过出版实验室手册和在线资源传播给更广泛的研究界。