CSHL Advanced Techniques In Molecular Neuroscience Course

CSHL 分子神经科学高级技术课程

• 批准号: 8934990
• 负责人: DAVID J. STEWART
• 金额: $ 10.57万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-06 至 2020-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8934990
• 关键词: Affinity Chromatography; Animal Viruses; Applied Research; Area; Attention; Basic Science; Biological Assay; Chromatin; Chromatin Structure; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; Disease; Educational Curriculum; Educational process of instructing; Electrophysiology (science); Electroporation; Ensure; Environment; Exercise; Faculty; Funding; Future; Gene Transfer; Genes; Home environment; Image; Individual; Infection; Institution; Laboratories; Left; Mainstreaming; Mammalian Cell; Mental Health; Messenger RNA; Methodology; Methods; Molecular; Molecular Neurobiology; Neurobiology; Neuroglia; Neurons; Neurosciences; Neurosciences Research; New York; Participant; Pharmacology; Physicians; Postdoctoral Fellow; Process; Publications; RNA Interference; Reporting; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Ribosomes; Role; Scientist; Series; Specific qualifier value; Students; Techniques; Time; Training; Training Programs; Transfection; Transgenic Organisms; Translating; United States; Update; Work; arm; base; career; design; experience; forging; gene delivery system; genome editing; graduate student; in vivo; instructor; interest; laboratory manuals; lecturer; medical specialties; member; nervous system disorder; programs; public health relevance; relating to nervous system; vector

 DESCRIPTION (provided by applicant): CSHL Laboratory Course in Advanced Techniques in Molecular Neuroscience The proposed Cold Spring Harbor Laboratory (CSHL) course Advanced Techniques in Molecular Neuroscience (ATMN), to be held annually in July 2015 - 2019, is part of the CSHL's postgraduate training program in neurobiology. ATMN is a short, intensive 2.5 week hands-on course which prepares students to enter directly into research that makes use of advanced and/or specialized molecular techniques in contemporary aspects of neurobiological research. Experimental modules in the course will include an introduction to the design and use of animal virus vectors in neuroscience research; the use of CRISPR genome editing and RNAi approaches for regulating the expression of specific genes in neurons and glia; practical exercises in gene delivery systems including mammalian cell infection and transfection and electroporation techniques for targeted gene transfer in vivo; an introduction to overall strategies, use and design of BAC transgenic vectors; real time RT-PCR analyses; assays of chromatin and chromatin structure in neurons, and mRNA isolation from specified neural subtypes by translating ribosome affinity purification. Experimental techniques are taught in the context of the broader conceptual advances that they may provide. The course invites lecturers who have made significant contributions in their fields to give up-to-the-minute reports on current research. The trainees are chosen by the course faculty from larger pools of applicants and range from graduate students to senior investigators and research physicians. Because of the short duration of this course, senior, as well as junior individuals can attend and receive a short, intense period of training in an environment remote from other demands on their time and attention. The ATMN course also provides an unusual opportunity for established scientists to apply these advanced techniques to their own research interests, or to retrain in an important specialty within neuroscience with which they may not be familiar. Methods and concepts taught in the course are disseminated to the wider research community through the publication of laboratory manuals and online resources.

 描述（由适用提供）：分子神经科学的高级技术实验室课程拟议的Cold Spring Harbour Laboratory（CSHL）分子神经科学（ATMN）的先进技术将于2015年7月 - 2019年7月至2019年举行，是CSHL神经生物学研究生培训计划的一部分。 ATMN是一项简短的2.5周动手课程，使学生直接进入研究，该研究利用神经生物学研究的当代方面使用先进和/或专业的分子技术。课程中的实验模块将包括对神经科学研究中动物病毒载体的设计和使用的介绍；使用CRISPR基因组编辑和RNAi方法来确定神经元和神经胶质中特定基因的表达；在基因递送系统中进行的实际练习，包括用于体内靶向基因转移的哺乳动物细胞感染以及转染和电穿孔技术； BAC转基因向量的整体策略，使用和设计的简介；实时RT-PCR分析；通过翻译核糖体亲和力纯化，神经元中染色质和染色质结构的测定，以及从指定神经元分离的mRNA。实验技术是在他​​们可能提供的更广泛的概念进步的背景下进行的。课程邀请讲师在其领域做出了重大贡献，以付出有关当前研究的最新报告。学员是由较大申请人池的课程教师选择的，范围从研究生到高级研究人员和研究医生。由于本课程的持续时间很短，因此高年级以及初中的人可以参加并在远离对其时间和关注的其他需求的环境中进行短暂而激烈的培训。 ATMN课程还为既定的科学家提供了不寻常的机会，可以将这些先进的技术应用于自己的研究兴趣，或者在神经科学中的重要专业中重新培训，他们可能不熟悉。课程中教授的方法和概念通过出版实验室手册和在线资源而传播到广泛的研究社区。