Monkey model of sarcopenia

肌肉减少症猴模型

• 批准号: 8931528
• 负责人: Julie Mattison
• 金额: $ 22.16万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8931528
• 关键词: Age; Aging; Animal Model; Area; Biochemical; Biological Assay; Biopsy; Data; Devices; Fiber; Freezing; Health Care Costs; Intervention; Intervention Studies; Macaca mulatta; Measures; Methods; Modeling; Monkeys; Mus; Muscle; Muscle Fibers; Muscle function; Myosin ATPase; Process; Rattus; Saimiri; Sampling; Testing; Therapeutic; Training; age related; arm; base; combat; disability; frailty; grasp; interest; muscle aging; muscle strength; muscular structure; nonhuman primate; sarcopenia; vastus lateralis; wasting

Vastus lateralis muscle biopsies have been collected from all of the squirrel monkeys. The samples were frozen for processing. Biochemical and histological analysis of the muscle samples is complete and the data are being analyzed. Assays include myosin ATPase activity for determination of muscle fiber type, proportions, and cross-sectional area. Preliminary data indicate an age-related decline in type II fibers, consistent with other species. A grip strength apparatus was developed and monkeys are currently being trained to use it. Data indicate a decrease in grip force with age. Grip force will provide a functional measure of muscle strength and an approximation of maximal grip force. As proof of principle for a disuse model, a monkey's arm was casted for 2 weeks. In subsequent grip testing, max force had decreased by approximately 30%. Further testing is planned.

从所有松鼠猴子收集了巨大的外侧肌肉活检。将样品冷冻以进行处理。肌肉样本的生化和组织学分析已完成，并且正在分析数据。测定包括用于测定肌肉纤维类型的肌球蛋白ATPase活性，比例和横截面区域。初步数据表明与其他物种一致的II型纤维与年龄相关的下降。开发了握力的设备，目前正在培训猴子来使用它。数据表明随着年龄的增长而减少握力。握力将提供肌肉强度和最大握力的近似值的功能度量。作为废弃模型原理的证明，猴子的手臂被铸造了2周。在随后的握力测试中，最大力量减少了约30％。计划进一步测试。