Monkey model of Alzheimer's disease

阿尔茨海默病的猴子模型

• 批准号: 8736543
• 负责人: Julie Mattison
• 金额: $ 2.28万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8736543
• 关键词: Accounting; Adult; Alzheimer' s Disease; Alzheimer' s disease model; Amyloid; Amyloid beta-Protein; Animal Model; Brain; Catecholamines; Chemicals; DSP 4; Deposition; Dopamine-beta-monooxygenase; Elderly; Female; Genes; Human; Immunohistochemistry; Injection of therapeutic agent; Lesion; Macaca mulatta; Manuscripts; Measurement; Monkeys; Mus; Neurotoxins; Pathology; Rodent; Saimiri; Sampling; Tissues; aged; beta-site APP cleaving enzyme 1; in vivo; locus ceruleus structure; mutant; neuroinflammation; nonhuman primate

Adult and aged female rhesus macaques and squirrel monkeys were injected with the neurotoxin DSP4 (N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine) or vehicle, with repeat injections after three and six months. At nine months post-initial treatment, brain samples were collected for measurement of catecholamines, amyloid precursor protein, soluble amyloid-Beta peptides (1-40 and 1-42) and Beta-site APP cleaving enzyme-1. Immunohistochemistry was used to assess amyloid distribution (6E10) and localization of dopamine Beta-hydroxylase in locus coeruleus sections. The in vivo portion of this study and all tissue analyses have been completed. The manuscript is nearing completion.

在三半月后重复注射成年和老年雌性猕猴和松鼠猴或猴子猴子（N-（2-氯乙基）-N-乙基-2-溴苯胺）或载体，三分之三和六个月后重复注射。在初始化后九个月，收集大脑样品，以测量儿茶酚胺，淀粉样蛋白前体蛋白，可溶性淀粉样蛋白-BETA肽（1-40和1-42）和Beta-Site App App Cleaeaving酶-1。 免疫组织化学用于评估淀粉样蛋白分布（6E10）和多巴胺β-羟化酶在基因座切片中的定位。 本研究的体内部分和所有组织分析均已完成。手稿即将完成。