Acquisition of Sony Biotechnology iCyt Synergy SY3200 BSC Cell Sorter

收购索尼生物技术 iCyt Synergy SY3200 BSC 细胞分选仪

• 批准号: 8639196
• 负责人: J. Michael MCCAFFERY
• 金额: $ 51.97万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-15 至 2015-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639196
• 关键词: Affect; Age; Area; Arts; Baltimore; Biology; Biotechnology; Cell Separation; Cells; Chemicals; Computers; Containment; Education; Educational Curriculum; Educational workshop; Engineering; Ensure; Funding; Head; Hospitals; Image; Laboratories; Letters; Maintenance; Microscopy; Recovery; Research Personnel; Resources; Schools; Science; Site; Sorting - Cell Movement; Specimen; System; Training; Universities; design; interest; oncology; operation

DESCRIPTION (provided by applicant): 13 investigators from The Johns Hopkins University, comprising the School of Arts & Sciences (and the Department of Biology); the Whiting School of Engineering (and the Department of Chemical and Biomolecular Engineering and the PS-OC Engineering in Oncology Center); and the Union Memorial Hospital and the Orthobiologic Laboratory request $519,686 to purchase a Sony Biotechnology Synergy SY3200 Cell sorter. This system will be sited in the Johns Hopkins' Integrated Imaging Center (IIC, ://www.jhu.edu/iic); and become the centerpiece of an upgraded BSL-2 cell sorting facility serving the diverse needs of the Hopkins' Homewood Campus and North Baltimore area generally. The IIC is a Homewood campus/Hopkins-wide microscopy resource, jointly supported by the Krieger School of Arts and Sciences and the Whiting School of Engineering (see Ball & Douglas letter); and utilized regularly by multiple schools and departments comprising >150 laboratories and >450 users. The new Synergy sorter will replace a BD FACSVantage SE with DIVA option whose antiquated design and complexity makes it difficult and costly to operate/use routinely; dangerous for the operator due to bio- containment concerns during sorting that necessarily limit sorting to BL-1 level specimens; and unreliable and problematic to maintain due to its age and lack of parts availability. The new iCyt Synergy SY3200 sorter incorporates a sorting head that is integrated into a Baker SterilGARD(R) lll hood making it particularly safe for the operator with the sorter operations/adjustments being affected remotely by computer. Nozzle exchange is simple and necessary alignments automated and computer controlled. Additionally, with the sorter head integrated into the Baker BSC, the design is particularly compact. When installed, the new system will provide a BSL-2 sorting capability not currently available in North Baltimore and on the Hopkins' Homewood campus. Currently, users must wait for weeks to gain access to sorters available at the Hopkins' East Baltimore or Bay View campuses located inconveniently five and ten miles away respectively. It will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance; it will be made freely available to all interested users Hopkins-wide through workshops & hands-on training; and it will be incorporated into the Center for Biotechnology Education's Masters in Biotechnology curriculum; and the IIC's annual undergraduate/graduate course offerings. (://www.jhu.edu/iic/academic.htm).

描述（由申请人提供）：来自约翰·霍普金斯大学的 13 名研究人员，包括艺术与科学学院（以及生物系）；怀廷工程学院（以及化学和生物分子工程系以及肿瘤学中心的 PS-OC 工程）；联合纪念医院和骨科生物实验室请求 519,686 美元购买索尼生物技术 Synergy SY3200 细胞分选机。该系统将位于约翰·霍普金斯大学综合成像中心（IIC，://www.jhu.edu/iic）；并成为升级后的 BSL-2 细胞分选设施的核心，满足霍普金斯大学霍姆伍德校区和北巴尔的摩地区的多样化需求。 IIC 是霍姆伍德校区/霍普金斯大学范围内的显微镜资源，由克里格艺术与科学学院和怀廷工程学院联合支持（参见 Ball & Douglas 的信件）；并被多个学校和部门定期使用，其中包括超过 150 个实验室和超过 450 个用户。新的 Synergy 分拣机将用 DIVA 选件取代 BD FACSVantage SE，后者过时的设计和复杂性使得日常操作/使用变得困难且成本高昂；由于分选过程中的生物防护问题，必然限制分选 BL-1 级样本，这对操作员来说是危险的；由于其老化和缺乏零件可用性，其不可靠且难以维护。新型 iCyt Synergy SY3200 分选机采用集成到 Baker SterilGARD(R) lll 罩中的分选头，使其特别安全 操作员的分拣机操作/调整受到计算机的远程影响。喷嘴更换非常简单，并且必要的对准是自动化且由计算机控制的。此外，由于分拣机头集成到 Baker BSC 中，设计特别紧凑。安装后，新系统将提供目前在北巴尔的摩和霍普金斯大学霍姆伍德校区尚不具备的 BSL-2 分拣功能。目前，用户必须等待数周才能使用霍普金斯东巴尔的摩校区或湾景校区的分拣机，这些校区分别距离五英里和十英里，不方便。它将纳入IIC现有的、完善的充值系统，以确保回收供应和维护资金；它将通过研讨会和实践培训免费提供给霍普金斯范围内所有感兴趣的用户；并将纳入生物技术教育中心的生物技术硕士课程；以及 IIC 的年度本科生/研究生课程。 (://www.jhu.edu/iic/academic.htm)。

项目成果

Differential HDAC6 Activity Modulates Ciliogenesis and Subsequent Mechanosensing of Endothelial Cells Derived from Pluripotent Stem Cells.

差异 HDAC6 活性调节纤毛发生和随后源自多能干细胞的内皮细胞的机械传感。

• DOI: 10.1016/j.celrep.2018.06.083
• 发表时间: 2018
• 期刊: Cell reports
• 影响因子: 8.8
• 作者: Smith,Quinton;Macklin,Bria;Chan,XinYi;Jones,Hannah;Trempel,Michelle;Yoder,MervinC;Gerecht,Sharon
• 通讯作者: Gerecht,Sharon

Mimicking lichens: incorporation of yeast strains together with sucrose-secreting cyanobacteria improves survival, growth, ROS removal, and lipid production in a stable mutualistic co-culture production platform.

• DOI: 10.1186/s13068-017-0736-x
• 发表时间: 2017
• 期刊: Biotechnology for biofuels
• 影响因子: 6.3
• 作者: Li T;Li CT;Butler K;Hays SG;Guarnieri MT;Oyler GA;Betenbaugh MJ
• 通讯作者: Betenbaugh MJ

E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid.

E-选择素介导的滚动促进胰腺癌细胞与透明质酸的粘附。

• DOI: 10.1096/fj.201700331r
• 发表时间: 2017
• 期刊: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
• 作者: Shea,DanielJ;Li,YiW;Stebe,KathleenJ;Konstantopoulos,Konstantinos
• 通讯作者: Konstantopoulos,Konstantinos

Compliant substratum guides endothelial commitment from human pluripotent stem cells.

• DOI: 10.1126/sciadv.1602883
• 发表时间: 2017-05
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Smith Q;Chan XY;Carmo AM;Trempel M;Saunders M;Gerecht S
• 通讯作者: Gerecht S

Histone H3 Threonine Phosphorylation Regulates Asymmetric Histone Inheritance in the Drosophila Male Germline.

• DOI: 10.1016/j.cell.2015.10.002
• 发表时间: 2015-11-05
• 期刊: Cell
• 影响因子: 64.5
• 作者: Xie J;Wooten M;Tran V;Chen BC;Pozmanter C;Simbolon C;Betzig E;Chen X
• 通讯作者: Chen X