Amersham Typhoon 9410 Variable Model Imager

Amersham Typhoon 9410 变量模型成像仪

• 批准号: 6877605
• 负责人: J. Michael MCCAFFERY
• 金额: $ 13.74万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877605
• 关键词: autoradiography; bioimaging /biomedical imaging; biomedical equipment purchase; biomedical equipment resource; fluorescence; imaging /visualization /scanning; luminescence; phosphorescence

DESCRIPTION (provided by applicant): A diverse user-group of 10 NIH funded investigators, within the Department of Biology at The Johns Hopkins University, request funds to purchase an Amersham 9410 Typhoon Variable Mode Imager to replace an outmoded 1993 Molecular Dynamics SF Model 455, which has been the primary workhorse for the department since 1993. This state of- the-art Typhoon phosophorimager will replace and constitute a significant upgrade from our older SF 455 system in terms of capabilities and, most importantly, reliability. Department investigators, all well funded through the NIH, work on a host of basic cell biological/biochemical related questions including, though not limited to, calcium signaling in yeast; helicases and bacterial conjugation, regulation of transcription and DNA binding by proteins, glycose phosphotransferase system, membrane organization and dynamics; microtubule-based motors; membrane trafficking; germ cell development and migration; sensory neuron development; and mechanisms of endocytosis. In the requested configuration, the 9410 phosophorimager will afford our disparate group of investigators a broad range of basic/advance capabilities for imaging/quantifying gel sandwiches, full-sized agarose and polyacrylamide gels, membranes, microplates, and microarrays. These capabilities required by our investigators are absent or deficient on our current SF 455 system, a limited format system. An even more critical concern in this submission pertains to the age-related unreliability of our older SF 455 system; and that Amersham has discontinued supplying service/parts to maintain the system in competent working condition. Particular worrisome is that the MD 455 design incorporates a unique, un-replacable integral computer design for which parts have been progressively more difficult to acquire, reinforcing our concern that in the near future the system will become un-repairable leaving us with no system. An additional major consideration relates to the rather costly/messy maintenance of the film processor and safe handling/disposal of photographic chemicals. Consequently, given the essential nature of gels/blots to our basic biological/biochemical research and the evolving use of microarray analysis in genomics/proteomics by department investigators; coupled with escalating concerns pertaining to reliability and performance limitations of our current system; we are seeking to upgrade our common-use facilities with the addition of the 9410 variable mode imager. The Typhoon 9410 will be conveniently located, administered, and maintained, in the Department's Integrated Imaging Center (IIC); and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.

描述（由申请人提供）：约翰·霍普金斯大学生物学系内的10位NIH资助的研究人员的多样化用户群，要求资金购买Amersham 9410台风可变模式图像师，以替换1993年过时的1993年分子动力学SF Model 455，这是该部门的主要工作，这是1993年以来的主要工作。就能力和最重要的是可靠性而言，从较旧的SF 455系统构成了重大升级。部门的调查人员都通过NIH提供了良好的资金，研究了许多基本的细胞生物学/生化相关问题，包括但不限于酵母中的钙信号传导；解旋酶和细菌结合，蛋白质的转录和DNA结合调节，糖磷酸转移酶系统，膜组织和动力学；基于微管的电动机；膜贩运；生殖细胞发育和迁移；感觉神经元发展；和内吞作用的机制。在请求的配置中，9410 phosophorimager将为我们的研究人员提供各种基本/预先的能力，用于成像/量化凝胶三明治，全尺寸的琼脂糖和聚丙烯酰胺凝胶，膜，微板，微板板和微阵列。我们的研究人员所需的这些功能缺乏或缺乏我们当前的SF 455系统，这是一种有限的格式系统。 在此提交中，更关注的问题与我们较旧的SF 455系统的年龄相关的不可靠性有关；而且，阿默瑟姆（Amersham）停止提供服务/零件，以维护系统的工作条件。特别令人担忧的是，MD 455设计结合了一种独特的，不可替代的积分计算机设计，逐渐更难获取零件，这加剧了我们的担忧，即在不久的将来，该系统将变得无法修复，使我们没有系统。另一个重大考虑是涉及胶片处理器的昂贵/凌乱维护以及摄影化学品的安全处理/处置。因此，鉴于我们的基本生物学/生化研究的凝胶/印迹的基本性质以及部门研究人员在基因组学/蛋白质组学中对微阵列分析的不断发展的使用；再加上与我们当前系统的可靠性和绩效限制有关的问题；我们正在寻求通过添加9410变量模式成像仪来升级通用设施。 台风9410将在该部门的集成成像中心（IIC）中方便地，管理和维护；它将被整合到IIC现有的，良好的充值系统中，以确保恢复用于供应和维护的资金。