Amersham Typhoon 9410 Variable Model Imager

Amersham Typhoon 9410 变量模型成像仪

• 批准号: 6877605
• 负责人: J. Michael MCCAFFERY
• 金额: $ 13.74万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877605
• 关键词: autoradiography; bioimaging /biomedical imaging; biomedical equipment purchase; biomedical equipment resource; fluorescence; imaging /visualization /scanning; luminescence; phosphorescence

DESCRIPTION (provided by applicant): A diverse user-group of 10 NIH funded investigators, within the Department of Biology at The Johns Hopkins University, request funds to purchase an Amersham 9410 Typhoon Variable Mode Imager to replace an outmoded 1993 Molecular Dynamics SF Model 455, which has been the primary workhorse for the department since 1993. This state of- the-art Typhoon phosophorimager will replace and constitute a significant upgrade from our older SF 455 system in terms of capabilities and, most importantly, reliability. Department investigators, all well funded through the NIH, work on a host of basic cell biological/biochemical related questions including, though not limited to, calcium signaling in yeast; helicases and bacterial conjugation, regulation of transcription and DNA binding by proteins, glycose phosphotransferase system, membrane organization and dynamics; microtubule-based motors; membrane trafficking; germ cell development and migration; sensory neuron development; and mechanisms of endocytosis. In the requested configuration, the 9410 phosophorimager will afford our disparate group of investigators a broad range of basic/advance capabilities for imaging/quantifying gel sandwiches, full-sized agarose and polyacrylamide gels, membranes, microplates, and microarrays. These capabilities required by our investigators are absent or deficient on our current SF 455 system, a limited format system. An even more critical concern in this submission pertains to the age-related unreliability of our older SF 455 system; and that Amersham has discontinued supplying service/parts to maintain the system in competent working condition. Particular worrisome is that the MD 455 design incorporates a unique, un-replacable integral computer design for which parts have been progressively more difficult to acquire, reinforcing our concern that in the near future the system will become un-repairable leaving us with no system. An additional major consideration relates to the rather costly/messy maintenance of the film processor and safe handling/disposal of photographic chemicals. Consequently, given the essential nature of gels/blots to our basic biological/biochemical research and the evolving use of microarray analysis in genomics/proteomics by department investigators; coupled with escalating concerns pertaining to reliability and performance limitations of our current system; we are seeking to upgrade our common-use facilities with the addition of the 9410 variable mode imager. The Typhoon 9410 will be conveniently located, administered, and maintained, in the Department's Integrated Imaging Center (IIC); and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.

描述（由申请人提供）：约翰·霍普金斯大学生物系内由 10 名 NIH 资助的研究人员组成的不同用户组请求资金购买 Amersham 9410 Typhoon 可变模式成像仪，以替换过时的 1993 年 Molecular Dynamics SF Model 455 ，自 1993 年以来一直是该部门的主要主力。这架最先进的台风Phosophorimager 将取代我们旧的 SF 455 系统，并在功能和最重要的可靠性方面进行重大升级。该部门的研究人员均通过 NIH 获得充足的资助，致力于研究一系列基本的细胞生物学/生化相关问题，包括但不限于酵母中的钙信号传导；解旋酶和细菌接合、蛋白质转录和 DNA 结合的调节、糖磷酸转移酶系统、膜组织和动力学；基于微管的马达；膜贩运；生殖细胞发育和迁移；感觉神经元发育；和内吞作用的机制。在所要求的配置中，9410 磷光成像仪将为我们不同的研究小组提供广泛的基本/高级功能，用于成像/定量凝胶三明治、全尺寸琼脂糖和聚丙烯酰胺凝胶、膜、微孔板和微阵列。我们的调查人员所需的这些功能在我们当前的 SF 455 系统（有限格式系统）上不存在或不足。 本次提交的一个更关键的问题涉及我们较旧的 SF 455 系统与年龄相关的不可靠性； Amersham 已停止提供服务/零件以维持系统处于合格的工作状态。特别令人担忧的是，MD 455 设计采用了独特的、不可更换的整体计算机设计，其零件越来越难以获得，这更加增强了我们的担忧，即在不久的将来该系统将变得无法修复，导致我们失去系统。另一个主要考虑因素涉及胶片冲洗机相当昂贵/混乱的维护以及照相化学品的安全处理/处置。因此，考虑到凝胶/印迹对于我们基础生物/生化研究的本质以及部门研究人员在基因组学/蛋白质组学中微阵列分析的不断发展的应用；加上对我们当前系统的可靠性和性能限制的担忧不断升级；我们正在寻求通过添加 9410 可变模式成像仪来升级我们的常用设施。 Typhoon 9410 将在国防部的综合成像中心 (IIC) 内方便地定位、管理和维护；并将其纳入IIC现有的、完善的充值系统中，以确保回收供应和维护资金。