Acquisition of GE Typhoon FLA9500 Variable Mode Imager

采购 GE Typhoon FLA9500 可变模式成像仪

基本信息

批准号：
9274438
负责人：
J. Michael MCCAFFERY
金额：
$ 14.67万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-03-01 至 2018-02-28
项目状态：
已结题

项目摘要

A diverse user-group of 9 NIH funded investigators, within the Krieger School of Arts & Sciences, the Whiting School of Engineering at The Johns Hopkins University, and the nearby Carnegie Institution, request funds to purchase a GE Typhoon FLA9500 Variable Mode Imager to complement, and ultimately replace, an aging and heavily used Typhoon 9410, which has been the primary workhorse for the Biology department and the entire Hopkins’ Homewood Campus since 2005. investigators, all well funded through the NIH, work on a host of basic cell biological/biomedical related questions including, though not limited to, regulation of transcription and DNA binding by proteins, metabolic signal transduction, DNA alkylation, membrane organization and dynamics;; DNA methylation detection;; and RNA- protein assembly. In the requested configuration, the FLA9500 phosophorimager will afford our disparate group of investigators a broad range of basic/advance capabilities for imaging/quantifying gel sandwiches, full-sized agarose and polyacrylamide gels, membranes, microplates, and microarrays. A critical angst that inspires this submission pertains to the age of our present Typhoon 9410 and concerns regarding future maintenance. We have been told that GE will not guarantee maintenance beyond 2018. Consequently, given the essential nature of quantitive gel blot and storage phosphor analysis to the basic research effort of our broader scientific community;; we are seeking to address this concern with the FLA9500 variable mode imager. The new Typhoon will be conveniently located, administered, and maintained, in the Johns Hopkins' Integrated Imaging Center (IIC);; and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.

Krieger艺术与科学学院内的NIH 9H资助调查人员的多样化用户组，Whiting School 约翰霍普金斯大学和附近卡内基机构的工程要求资金购买GE 台风FLA9500可变模式成像仪将完成，并最终替换，吞噬和天上使用的台风 9410，它一直是生物学部门和整个霍普金斯的霍姆伍德校园的主要主力军自2005年以来，研究人员都通过NIH资助了大量资金蛋白质的转录和DNA结合的调节，包括蛋白质，代谢信号，包括但不限于转录和DNA结合的问题转导，DNA烷基化，膜组织和动力学； DNA甲基化检测；和RNA- 蛋白质组件。在请求的配置中，FLA9500 phosophorimager将负担我们不同的组调查人员有多种基本/提前功能，用于成像/量化凝胶三明治，全尺寸琼脂糖和聚丙烯酰胺凝胶，膜，微板和微阵列。一种启发这一提交的关键焦虑与我们目前的台风9410的年龄有关，并涉及常规未来的维护。我们被认为是GE不会保证在2018年以后的维护。因此，定量凝胶印迹和储存磷酸分析的基本性质，我们广播公司科学的基础研究工作社区;;我们希望通过FLA9500可变模式成像仪来解决这一问题。新台风将在约翰·霍普金斯（Johns Hopkins）的集成成像中方便地，管理和维护中心（IIC）;;它将被整合到IIC现有的，已建立的充电系统中，以确保恢复用于供应和维护的资金。