Zeiss/31 Stallion DDI Multi-Dimensional Live-Cell Imaging Workstation

Zeiss/31 Stallion DDI 多维活细胞成像工作站

• 批准号: 7046510
• 负责人: J. Michael MCCAFFERY
• 金额: $ 30.35万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7046510
• 关键词: aging; apoptosis; biotechnology; cell adhesion; centromere; complement; digital imaging; endocytosis; fasting; fluorescence; genes; lasers; lysosomes; medicine; membrane biogenesis; microscopy; mitochondria; molecular /cellular imaging; neoplasm /cancer; particle; photoactivation; public health; schools; telomere; university; vesicle /vacuole

DESCRIPTION (provided by applicant): A diverse group of 11 NIH funded investigators, within the Johns Hopkins Schools of Arts and Sciences, Engineering, Medicine, and Public Health; and the University of Maryland Biotechnology Institute in the University of Maryland School of Medicine request funds to purchase a Stallion DPI Live-Cell digital Imaging workstation from Carl Zeiss/lntelligent Imaging Innovations. The requested system integrates a Zeiss 200M TIRF-ready platform with dual ultra-fast/highly sensitive Cascade 512B EM digital cameras; a 405/488 FRAP/photoactivation laser module; fully integrated 31 Slidebook multidimensional acquisition/analysis software; and is optimized for 1) rapid/ultra-sensitive 4D two channel acquisition; 2) deconvolution; 3) FRET and ratio imaging; 4) particle tracking; and 5) FRAP and photoactivation. The new state-of-the-art workstation will complement our heavily utilized Zeiss LSM 510 METAs and aging Deltavision system, while constituting a significant upgrade in speed and sensitivity. Importantly, the Stallion DPI will provide new/unique widefield fluorescence capabilities for the Hopkins' Integrated Imaging Center, a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >60 laboratories and ~200 users. Our investigators, all well funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: telomere/centromere reorganization in tumors; HSV assembly and maturation; MHC I trafficking and assembly, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; mitochondria and apoptosis; sialic acid display and cell adhesion; and mechanisms of endocytosis. In the requested configuration, the Stallion DDI workstation will afford our disparate group of investigators a broad range of basic/advanced live-cell microscopy capabilities that are presently deficient/absent from the Hopkins Homewood campus. The new system will be conveniently located, administered, and maintained, in the Biology Department's Integrated Imaging Center (NO: and it will be incorporated into the IIC's existing, well established recharge system to ensure recovery of funds for supplies and maintenance.

描述（由申请人提供）：约翰·霍普金斯艺术和科学，工程，医学和公共卫生的约翰·霍普金斯学院内的11位NIH资助的研究人员组成的多元化组；马里兰大学医学院的马里兰大学生物技术学院要求资金从Carl Zeiss/Lntelligent Imaging Intovations购买种马DPI DPI Live-Cell数字成像工作站。所请求的系统将Zeiss 200M TIRF就绪平台与双超快速/高敏感的Cascade 512B EM数码相机相结合； 405/488 FRAP/光激活激光模块；完全集成的31幻灯簿多维获取/分析软件；并针对1）快速/超敏感的4D两个通道采集进行了优化； 2）反卷积； 3）fret和比率成像； 4）粒子跟踪；和5）手法和光激活。新的最先进的工作站将补充我们大量利用的Zeiss LSM 510 Metas和老化的三角车系统，同时构成了速度和灵敏度的重大升级。重要的是，种马DPI将为霍普金斯的集成成像中心提供新/独特的广场荧光功能，这是一个霍姆伍德校园/霍普金斯范围内的显微镜资源，由多个由60个实验室和〜200用户组成的学校和部门定期使用。我们的研究人员都通过NIH提供了充分的资金，从事许多基本细胞生物学，生化和生物分子相关的问题，包括但不限于：肿瘤中的端粒/Centromere重组； HSV组装和成熟； MHC I贩运和集会，膜组织和动态；内体/溶酶体动力学；膜贩运；聚合基因载体的贩运；线粒体和凋亡；唾液酸显示和细胞粘附；和内吞作用的机制。在要求的配置中，种马DDI工作站将为我们不同的调查人员提供多种基本/先进的活细胞显微镜功能，目前从Hopkins Homewood校园中缺乏/缺乏。新系统将在生物部集成成像中心中方便地，管理和维护（否：它将被整合到IIC现有的，良好的充值系统中，以确保恢复用于供应和维护的资金。