LBRN: MOLECULAR CELL BIOLOGY CORE

LBRN：分子细胞生物学核心

• 批准号: 7959459
• 负责人: Konstantin G Kousoulas
• 金额: $ 12.25万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959459
• 关键词: Animals; Apoptosis; Area; Arts; Bioinformatics; Biological; Biomedical Research; Biotechnology; Cancer Biology; Cell Aging; Cell Cycle; Cell Differentiation process; Cells; Cellular biology; Centers of Research Excellence; Communication; Computer Retrieval of Information on Scientific Projects Database; Confocal Microscopy; Development; Developmental Biology; Electrons; Ensure; Equipment; Funding; Genetic; Genome; Genomics; Goals; Grant; Human; Human Resources; Immunology; Individual; Infectious Diseases Research; Institution; Louisiana; Methods; Mining; Molecular; Molecular Biology; Molecular Medicine; National Center for Research Resources; Natural Products Chemistry; Neurobiology; Organism; Physiological; Production; Proteins; Proteomics; Research; Research Personnel; Research Support; Resources; Schools; Science; Services; Source; Structure; Technology; Training; Training Support; United States National Institutes of Health; Universities; Veterinary Medicine; base; cell age; environmental toxicology; improved; innovation; interest; molecular pathology; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Original Rationale and Specific Aims Molecular Cell Biology provides an essential linkage among important basic fields of biomedical sciences, such as genetics, developmental biology, immunology, neurobiology and cancer biology. In recent years, the application of molecular biological principles and methods has made it possible to vastly improve our understanding of various cellular mechanisms at cellular and subcellular levels including the phenomena of cell cycle, cell differentiation, cell development, cell aging and apoptosis. The elucidation of the human and other animal genomes in conjunction with analysis of all forms of the encoded proteins will soon enable the identification of all the gene products that are potentially synthesized by an organism. The vast information generated by genomic sequencing and proteomic analysis can be effectively mined only by advanced biocomputation/bioinformatics approaches, which will ultimately, connect sequence information (genomics) and protein identification (proteomics) to physiological function within the context of the cell. Therefore, molecular cell biology is at the center of a new era of biomedical research. We have chosen the theme of Molecular Cell Biology as an all encompassing theme that includes the broad research interests of all participating institutions, as well as the specific interests of the assembled cadre of junior investigators. For example, the PUI investigators at Southern University focus on molecular and environmental toxicology, as well as cancer biology, while those at the University of Louisiana at Monroe study natural products chemistry and neurobiology. Based on institutional goals, it is clear that additional new investigators will be added within participating institutions to enhance their molecular cell biology programs. To ensure ample personnel training and support of individual projects, as well as to facilitate effective coordination and communication among all researchers including coordination and communication with bioinformatics/biocomputation researchers, we have established a Molecular and Cell Biology Core (MCBC). Since its inception, the INBRE MCBC took advantage of the technological strengths and state-of-the-art molecular facilities of a Molecular Biology and Immunology Core (LSU-MBIC) of the LSU-Tulane COBRE in Experimental Infectious Disease Research (PI, K. G. Kousoulas). This Core is located within and administered by the Division of Biotechnology and Molecular Medicine (BIOMMED) at the LSU School of Veterinary Medicine (see BIOMMED description below). The integration of the LSU COBRE-MBIC and the INBRE MCBC under the direction of BIOMMED was intended to leverage and expand the available resources and expertise of each unit and to synergistically enhance the function of the two cores, while minimizing the duplication of equipment and services. The COBRE/INBRE MCBC has evolved over the last few years to encompass additional service functions including molecular pathology, electron and confocal microscopy, FACS and protein production and purification. In this regard MCBC has been able to coordinate and facilitate research support provided by other LSU-based centralized facilities to structure the greatest possible intellectual platform upon which innovative and creative ideas will merge with state-of-the-art technologies. This INBRE/COBRE resource cross-utilization constitutes NCRR programmatic goals that can effectively enhance statewide research competitiveness in the biomedical sciences. The specific aims of the MCBC are: Specific Aim I: To support the research efforts and activities of the INBRE investigators by providing advanced molecular cell biology training and access to state-of-the-art equipment. Specific Aim II: To train and develop a critical mass of investigators who will expand the potential for extramurally-funded molecular cell biology research in PUI campuses. Specific Aim III: To coordinate and facilitate interactions among MCBC and other INBRE investigators, as well as between INBRE investigators and other senior NIH-funded investigators. Specific Aim IV: To coordinate and facilitate collaborative research endeavors between researchers of the MCBC and BBC thematic areas of the INBRE.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 最初的理由和具体目标 分子细胞生物学在生物医学科学的重要基础领域（如遗传学、发育生物学、免疫学、神经生物学和癌症生物学）之间提供了重要的联系。近年来，分子生物学原理和方法的应用，极大地提高了我们对细胞和亚细胞水平上各种细胞机制的理解，包括细胞周期、细胞分化、细胞发育、细胞衰老和凋亡等现象。对人类和其他动物基因组的阐明以及对所有形式的编码蛋白质的分析将很快能够鉴定有机体可能合成的所有基因产物。基因组测序和蛋白质组分析产生的大量信息只能通过先进的生物计算/生物信息学方法才能有效地挖掘，这些方法最终将序列信息（基因组学）和蛋白质鉴定（蛋白质组学）与细胞背景下的生理功能联系起来。因此，分子细胞生物学是生物医学研究新时代的中心。 我们选择分子细胞生物学作为一个包罗万象的主题，其中包括所有参与机构的广泛研究兴趣，以及初级研究人员骨干的具体兴趣。例如，南方大学的 PUI 研究人员专注于分子和环境毒理学以及癌症生物学，而路易斯安那大学门罗分校的研究人员则研究天然产物化学和神经生物学。根据机构目标，很明显，参与机构内将增加更多的新研究人员，以加强其分子细​​胞生物学项目。为了确保充足的人员培训和单个项目的支持，以及促进所有研究人员之间的有效协调和沟通，包括与生物信息学/生物计算研究人员的协调和沟通，我们建立了分子和细胞生物学核心（MCBC）。 自成立以来，INBRE MCBC 在实验传染病研究（PI、K. G.库苏拉斯）。该核心位于路易斯安那州立大学兽医学院生物技术和分子医学 (BIOMMED) 部门并由其管理（参见下面的 BIOMMED 描述）。在 BIOMMED 的指导下，路易斯安那州立大学 COBRE-MBIC 和 INBRE MCBC 的整合旨在利用和扩展每个单位的可用资源和专业知识，协同增强两个核心的功能，同时最大限度地减少设备和服务的重复。 COBRE/INBRE MCBC 在过去几年中不断发展，涵盖了额外的服务功能，包括分子病理学、电子和共聚焦显微镜、FACS 以及蛋白质生产和纯化。在这方面，MCBC 能够协调和促进其他位于路易斯安那州立大学的集中设施提供的研究支持，以构建尽可能最大的智力平台，在此基础上创新和创意思想将与最先进的技术相融合。这种 INBRE/COBRE 资源交叉利用构成了 NCRR 的规划目标，可以有效增强全州生物医学科学的研究竞争力。 MCBC 的具体目标是： 具体目标 I：通过提供先进的分子细胞生物学培训和最先进的设备，支持 INBRE 研究人员的研究工作和活动。 具体目标 II：培训和培养一批关键的研究人员，他们将扩大 PUI 校园内外部资助的分子细胞生物学研究的潜力。 具体目标 III：协调和促进 MCBC 和其他 INBRE 研究人员之间以及 INBRE 调查人员和 NIH 资助的其他高级研究人员之间的互动。 具体目标 IV：协调和促进 MCBC 和 BBC INBRE 主题领域的研究人员之间的合作研究工作。