Molecular Biology and Immunopathology Core

分子生物学和免疫病理学核心

基本信息

批准号：
10360594
负责人：
Konstantin G Kousoulas
金额：
$ 30.67万
依托单位：
LOUISIANA STATE UNIV A&M COL BATON ROUGE
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-03-01 至 2026-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10360594
关键词：
Adopted Bioinformatics Biological Biological Assay Biomedical Research Biotechnology CRISPR/Cas technology Cell Culture Techniques Cells Cellular biology Centers of Research Excellence Collaborations Computer software Computers and Advanced Instrumentation Consult Consultations Contract Services Core Facility Data Disease Disease model Electron Microscopy Ensure Equipment Experimental Designs Faculty Faculty Recruitment Funding Gene Expression Genomics Genus Hippocampus Goals Human Human Resources Immunofluorescence Microscopy Immunologics Immunology Investigation Laboratories Leadership Life Louisiana Maintenance Malignant Neoplasms Measurement Measures Methodology Methods Molecular Molecular Biology Molecular Medicine Monoclonal Antibodies Pathology Patients Productivity Proteins Proteomics Quantitative Reverse Transcriptase PCR Reagent Research Research Personnel Resources Reverse Transcriptase Polymerase Chain Reaction Science Services Students Technical Expertise Technology Time Tissue Sample Training Training Activity Training and Education Treatment outcome United States National Institutes of Health Universities Update Veterinary Medicine Veterinary Schools Viral Vector Work animal imaging animal tissue anticancer research base bioimaging cost course module experimental study immunopathology in vivo instrumentation interest nano-string new technology novel therapeutic intervention pre-clinical preclinical evaluation predictive modeling repaired single cell sequencing transcriptome sequencing transcriptomics

项目摘要

Summary Molecular Biology Immunopathology Core. The proposed Center for Pre-Clinical Cancer Research (Cancer COBRE) at Louisiana State University (LSU) focuses on elucidating molecular and cellular mechanisms of human cancer using specialized disease models that are predictive of disease in humans. The Cancer COBRE will focus on devastating human cancers that have poor treatment outcome in patients in need of novel therapeutic strategies. The proposed projects led by highly Promising Junior Investigators (PJIs) require the application of advanced molecular biological and immunopathological principles, methods and particularly advances in genomics, transcriptomics and proteomics, immunology and pathology to delineate key cellular mechanisms for each specific cancer necessitating access to state-of-the-art equipment and technical expertise. The Molecular Biology and Immunopathology Core (MBIPC) has been created to leverage and expand the resources and expertise of the Division of Biotechnology and Molecular Medicine (BioMMED) at the LSU School of Veterinary Medicine (SVM), which administers six Core Facilities: Gene Expression and Sequencing Laboratory (GeneLab), Viral Vectors Laboratory, Protein Characterization and Purification laboratory (PCPL), which is also generates monospecific and monoclonal antibodies to proteins of interest, the Small Animal Imaging Laboratory (SAIL) and the Molecular Immunopathology laboratory (MIPL) that provides expertise in immunopathology and access to the SVM Centralized Facilities (FACS and Pathology Cost Centers), and also provides expertise and access to bioimaging and advanced immunofluorescence and electron microscopy. These laboratories are currently co-supported by the Louisiana Biomedical Research Network (LBRN/INBRE), which also operates the LBRN Bioinformatics Core in close collaboration with the LSU Center for Computation & Technology (CCT). The goal of MBIPC is to provide and coordinate access to state of the advanced instrumentation and services and provide training in a broad spectrum of fundamental and advanced molecular biology and immunopathology to all PJIs and other NIH- funded investigators to achieve their research objectives. MBIPC has identified two specific aims: Aim 1. To provide the Cancer COBRE and other NIH-funded investigators with resources and expertise in molecular and cell biology and immunopathology including instrumentation, technical support, consultation and training, and assistance in developing new methodologies. Aim 2: To organize, coordinate, and facilitate interactions among COBRE investigators and work collaboratively to develop and use new technologies and assays, and train PJIs, faculty, staff, and students in advanced molecular biology and immunopathology technologies.

分子生物学免疫病理学核心摘要。拟议的路易斯安那州立大学 (LSU) 临床前癌症研究中心 (Cancer COBRE) 专注于利用专门的疾病模型阐明人类癌症的分子和细胞机制可以预测人类疾病。癌症 COBRE 将重点关注毁灭性的人类癌症需要新治疗策略的患者治疗效果不佳。拟议的项目由高度领导领导有前途的初级研究者 (PJI) 需要应用先进的分子生物学和免疫病理学原理、方法，特别是基因组学、转录组学和蛋白质组学的进展，免疫学和病理学来描绘每种需要访问的特定癌症的关键细胞机制最先进的设备和技术专长。分子生物学和免疫病理学核心 (MBIPC) 的成立是为了利用和扩展生物技术部门的资源和专业知识路易斯安那州立大学兽医学院 (SVM) 的分子医学 (BioMMED) 管理着六个核心设施：基因表达与测序实验室（GeneLab）、病毒载体实验室、蛋白质表征和纯化实验室 (PCPL)，也生成单特异性和单克隆感兴趣的蛋白质抗体，小动物成像实验室 (SAIL) 和分子免疫病理学实验室 (MIPL)，提供免疫病理学专业知识和 SVM 访问权限集中设施（FACS 和病理学成本中心），还提供专业知识和生物成像的途径以及先进的免疫荧光和电子显微镜。这些实验室目前得到以下机构的共同支持：路易斯安那生物医学研究网络 (LBRN/INBRE)，该网络还运营着 LBRN 生物信息学核心与路易斯安那州立大学计算与技术中心 (CCT) 密切合作。 MBIPC 的目标是提供协调先进仪器和服务的获取，并提供广泛的培训涵盖所有 PJI 和其他 NIH 的基础和高级分子生物学和免疫病理学资助研究人员实现他们的研究目标。 MBIPC 确定了两个具体目标：目标 1. 为癌症 COBRE 和其他 NIH 资助的研究人员提供以下方面的资源和专业知识：分子和细胞生物学和免疫病理学，包括仪器、技术支持、咨询和培训和协助开发新方法。目标 2：组织、协调和促进 COBRE 研究人员之间的互动和协作开发和使用新技术和检测方法，并对 PJI、教职员工和学生进行高级培训分子生物学和免疫病理学技术。