Molecular Biology

分子生物学

• 批准号: 10341064
• 负责人: Konstantin G Kousoulas
• 金额: $ 22.02万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-02 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10341064
• 关键词: Acute Lung Injury; Address; Allergens; Antibodies; Biological; Biological Assay; Biology; Biomedical Research; Biotechnology; Cells; Cellular biology; Centers of Research Excellence; Computer software; Consult; Consultations; Contract Services; Core Facility; Data; Data Analyses; Development; Disease; Electron Microscopy; Endotoxins; Ensure; Equipment; Experimental Designs; Extrinsic asthma; Faculty; Faculty Recruitment; Funding; Future; Gene Chips; Gene Expression; Gene Expression Profiling; Generations; Genomics; Genotype; Goals; Human; Human Genome; Immunofluorescence Microscopy; Immunologics; Infection; Inflammation; Influenza; Institution; Kidney; Laboratories; Liver; Louisiana; Lung; Lung diseases; Maintenance; Measures; Mediating; Mentors; Methodology; Methods; Molecular; Molecular Biology; Molecular Biology Techniques; Molecular Medicine; Mus; National Institute of General Medical Sciences; Organ; Pathogenesis; Phase; Plasmid Cloning Vector; Preparation; Productivity; Proteins; Proteomics; Quantitative Reverse Transcriptase PCR; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Sampling; Sepsis; Septic Shock; Services; Students; Technical Expertise; Technology; Training; Training and Education; Tuberculosis; United States National Institutes of Health; Universities; Update; Veterinary Medicine; Veterinary Schools; Viral Vector; assault; bioimaging; biomarker validation; cecal ligation puncture; experience; immunopathology; improved; inflammatory lung disease; instrumentation; interest; microbial; mouse genome; mouse model; nano-string; new technology; repaired; response; single cell sequencing; transcriptome sequencing; transcriptomics

PROJECT SUMMARY The Molecular Biology (MB) Core has been created to meet the need for advanced molecular biology support by PJIs. In particular, access to and experience in utilizing advanced molecular biology that are limited for most PJIs and established NIH-funded investigators. Specifically, MB Core will leverage and expand the resources and expertise of the Genelab Core facility which is comprised of three existing Core Facilities operated by the Division of Biotechnology and Molecular Medicine (BioMMED) at the LSU School of Veterinary Medicine (SVM): Gene expression and sequencing Laboratory (GeneLab), Protein characterization and purification laboratory (PCPL), which is also responsible for generation of monospecific antibodies to proteins of interest, and the Molecular Immunopathology laboratory (MIPL) that provides expertise and access to bioimaging and advanced immunofluorescence and electron microscopy. These three laboratories are currently co-supported by the NIGMS-funded CEIDR-COBRE (Phase III) as well as the Louisiana Biomedical Research Network (LBRN/INBRE). The goal of the MB Core Facility of the Lung COBRE is to provide high quality instrumentation, sample preparation and training in a broad spectrum of fundamental and advanced molecular biology techniques to all COBRE PJIs and other NIH-funded investigators to achieve their research objectives. The MB Core has established 2 specific aims. Aim 1 is to provide the COBRE and other NIH-funded investigators with resources and expertise in molecular and cell biology, including instrumentation, technical support, consultation, and training. Aim 2 is to organize, coordinate, and facilitate interactions among COBRE investigators, mentors, and students, promote the development and use of new technologies and assays, and train PJI faculty, staff, and students in advanced molecular biology technologies.

项目摘要 已经创建了分子生物学（MB）核心，以满足对高级分子生物学支持的需求 由PJIS。特别是，使用和使用高级分子生物学的访问和经验，这些生物学受到限制 PJI并建立了NIH资助的研究人员。具体而言，MB核心将利用和扩展资源 Genelab核心设施的专业知识，该设施由三个现有的核心设施组成 LSU兽医学院的生物技术与分子医学系（BIOMMED） （SVM）：基因表达和测序实验室（GenElab），蛋白质表征和纯化 实验室（PCPL）也负责生成对感兴趣蛋白质的单特异性抗体， 以及分子免疫病理学实验室（MIPL），可提供专业知识并获得生物成像和获取 晚期免疫荧光和电子显微镜。这三个实验室目前是共同支持的 由NIGMS资助的Ceidr-Cobre（第三阶段）以及路易斯安那州生物医学研究网络 （lbrn/inbre）。肺部COBRE的MB核心设施的目标是提供高质量的仪器， 在广泛的基本和晚期分子生物学中的样品制备和训练 所有COBRE PJIS和其他由NIH资助的研究人员实现其研究目标的技术。 MB 核心已经建立了2个具体目标。 AIM 1是向毛病和其他NIH资助的调查人员提供 分子和细胞生物学的资源和专业知识，包括仪器，技术支持， 咨询和培训。 AIM 2是组织，协调和促进毛绒之间的相互作用 调查人员，导师和学生，促进新技术和测定的开发和使用，以及 培训PJI高级分子生物学技术的PJI教职员工和学生。