FEI Tecnai F20 Transmission Electron Microscope for Core Microscopy Facility

用于岩芯显微设备的 FEI Tecnai F20 透射电子显微镜

• 批准号: 8733931
• 负责人: J. Michael MCCAFFERY
• 金额: $ 128.92万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2015-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8733931
• 关键词: Arts; Biochemical; Biological; Biology; Cell Adhesion; Cells; Chromosomes; Communities; Complement; Cryoelectron Microscopy; DNA; Detection; Dose; Electron Microscope; Endocytosis; Endosomes; Engineering; Ensure; Free Will; Funding; Future; Genes; Growth; HIV; Housing; Image; Institutes; Internet; Laboratories; Letters; Lysosomes; Maintenance; Membrane; Membrane Protein Traffic; Microscopy; Minor; Molecular; Recovery; Regulation; Research; Research Personnel; Resources; Schools; Science; Services; Sialic Acids; Simplexvirus; Structure; System; Telephone; United States National Institutes of Health; Work; interest; medical schools; nanobiotechnology; oncology; particle; trafficking; transmission process

DESCRIPTION (provided by applicant): A diverse group of 9 major and 8 minor users who are well funded NIH investigators, within the Johns Hopkins School of Arts & Sciences, Whiting School of Engineering, and the JHMI School of Medicine, request funds to purchase an FEI Tecnai F20 TWIN 200kV transmission electron microscope (TEM) with a Falcon II direct detection camera. This new state-of-the-art TEM will be housed in the Hopkins Integrated Imaging Center (www.jhu.edu/IIC) that is the only TEM facility on the Hopkins' Homewood campus; and is well utilized by the Hopkins-wide research community. It will replace a 30 year old Philips EM 420 for which service and day-to-day reliability has become critically dubious (see letter from John Fike, East Regional Service Manager, FEI in Appendix A); and perfectly complement an existing Tecnai 12. If funded, the Tecnai F20 will become the primary TEM platform for cryoelectron microscopy and low dose applications at Hopkins. Its 200kV capability, FEG, and Falcon II direct detection camera will add upgraded capabilities in cryo and molecular TEM, that are not currently present, astonishingly, anywhere at Hopkins; and it will free up our existing T12 for use in more conventional, routine TEM applications. Our investigators, all well-funded through the NIH, work on a host of basic cell biological, biochemical, and biomolecular related questions including, though not limited to: HIV structure and function, HSV assembly and maturation; DNA regulation and chromosome suprastructure, membrane organization and dynamics; endosome/lysosome dynamics; membrane trafficking; trafficking of polymeric gene carriers; sialic acid display and cell adhesion; and mechanisms of endocytosis. The new Tecnai F20 will ensure that our diverse group of investigators have continued and uninterrupted access to a TEM optimized for many disparate applications; as well as allowing for future growth in more exotic structural applications of cryoTEM and single particle analysis. The Tecnai F20 will be conveniently located, administered, and maintained, in the Biology Department's soon to be renovated Integrated Imaging Center (IIC), a Homewood campus/Hopkins-wide microscopy resource that is utilized regularly by multiple schools and departments comprising >150 laboratories and >400 users. The IIC is a Hopkins' Homewood campus core microscopy resource associated with the Institute for NanoBioTechnology (INBT); and the newly established Engineering in Oncology Center. It is jointly supported by the Krieger School of Arts and Sciences and the Whiting School of Engineering; and is utilized regularly by multiple schools/departments The new system will be incorporated into the IIC's existing recharge system to ensure recovery of funds for supplies and maintenance; and it will be made freely available to all interested users Hopkins-wide through our convenient IIC web-scheduler and smart phone app.

描述（由申请人提供）：约翰·霍普金斯艺术与科学学院，惠廷工程学院和JHMI医学学院内的9个专业和8个未次要用户的多元化组，他们是资助良好的NIH调查员带有Falcon II直接检测摄像头的Fei Tecnai F20 Twin 200KV传输电子显微镜（TEM）。这个新的最先进的TEM将安置在霍普金斯集成成像中心（www.jhu.edu/iic）中，该中心是霍普金斯家族校园唯一的TEM设施；并被霍普金斯范围内的研究社区充分利用。它将取代30岁的飞利浦EM 420，为此，服务和日常可靠性已变得非常怀疑（请参阅附录A的FEI的East Regional Service Servication Manager的信函）；并完美地补充了现有的tecnai 12。如果资助，Tecnai F20将成为霍普金斯（Hopkins）冷冻电子显微镜和低剂量应用的主要TEM平台。它的200kV功能，FEG和Falcon II直接检测摄像机将在霍普金斯的任何地方都添加升级的冷冻和分子TEM的升级功能。它将释放我们现有的T12，以用于更常规的常规TEM应用程序。我们的研究人员都通过NIH资金充分资助，致力于许多基本细胞生物学，生化和生物分子相关的问题，包括但不限于：HIV结构和功能，HSV组装和成熟； DNA调节和染色体上建筑，膜组织和动力学；内体/溶酶体动力学；膜贩运；聚合基因载体的贩运；唾液酸显示和细胞粘附；和内吞作用的机制。新的Tecnai F20将确保我们多样化的调查人员持续且不间断地访问了针对许多不同应用程序优化的TEM；以及允许在冷冻物和单个颗粒分析的更外来结构应用中实现未来的增长。 Tecnai F20将在生物学部门的综合成像中心（IIC）中方便地，管理和维护，这是Homewood校园/霍普金斯范围内的显微镜资源，该资源由多个学校和部门定期使用，由多个学校和部门> 150个实验室组成和> 400个用户。 IIC是霍普金斯的Homewood校园核心显微镜资源，与纳米型技术研究所有关（INBT）；以及新成立的肿瘤学中心工程。它得到了克里格艺术与科学学院和惠廷工程学院的共同支持；并经常被多个学校/部门使用，新系统将纳入IIC现有的充值系统中，以确保收回用于供应和维护的资金；它将通过我们方便的IIC网络制定器和智能手机应用程序免费提供给所有感兴趣的用户霍普金斯范围内的用户。