Genomics Shared Resource

基因组学共享资源

• 批准号: 8369395
• 负责人: MICHELE CARBONE
• 金额: $ 3.75万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8369395
• 关键词: Accounting; Archives; Area; Basic Science; Biological Assay; Blood; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cancer Etiology; Cell Fraction; Cells; Clinical Research; Complementary DNA; Consultations; DNA; DNA Resequencing; Epidemiologic Studies; Faculty; Fee-for-Service Plans; Fees; Freezing; Gene Chips; Gene Expression; Genes; Genetic Heterogeneity; Genomics; Genomics Shared Resource; Genotype; Goals; Grant; Hawaii; Human Genome; Human Resources; Information Services; Institutional Review Boards; Knowledge; Laboratories; Messenger RNA; Methods; Methylation; MicroRNAs; Mission; Molecular Biology; Molecular Genetics; Mutation; Nucleic Acids; Peer Review; PicoGreen; Population; Preparation; Prevention; Price; RNA; RNA chemical synthesis; Research; Research Personnel; Resources; Saliva; Sampling; Services; Small RNA; Time; Tissues; Translating; Universities; Wages; Whole Blood; base; bisulfite; cost; gel electrophoresis; genome-wide; high throughput technology; member; nano

The Genomics Shared Resource (GSR) is a core laboratory that has been supported in part by the CCSG since 1999. The purpose of the GSR laboratory is to provide expertise and services in the fast developing area of genomic analysis. During the current grant period, the GSR has expanded its services from genotyping to a number of other genomic analyses. Currently, the GSR provides nucleic acid isolation and quality analysis, genotyping, and also gene expression, copy number alteration and methylation analysis services in support of the peer-reviewed and IRB-approved research conducted by University of Hawai'i (UH) Cancer Center members. A variety of assays are performed on samples from cancer biology studies as well as on those collected in epidemiological and clinical studies that take advantage of the genetic heterogeneity of multiethnic populations, especially from Hawai'i. The scale of these studies varies from a few to tens of thousands of samples, the focus ranges from a single SNP or gene to genome-wide, and the assays performed on each sample range from one to over a million. The goal is to translate information now available on the human genome into new knowledge about cancer etiology, prevention and treatment. With the addition of several microarray platforms the GSR has significantly extended the range of its services and is serving a larger number of users (including basic science investigators) at UH Cancer Center and The Consortium. The GSR reduces redundancies at the Center by furnishing a central service that uses cutting edge and high-throughput technologies. In addition, the personnel of the GSR can provide project planning advice and troubleshooting capabilities during the execution of the projects. The personnel of the GSR are highly knowledgeable in the areas of molecular biology and genetics, and can provide both the novice and the advanced users with a level of technical support, consultation and information services that are not found elsewhere in Hawai'i.

基因组学共享资源 (GSR) 是一个核心实验室，自 1999 年以来一直得到 CCSG 的部分支持。GSR 实验室的目的是在快速发展的基因组分析领域提供专业知识和服务。在当前资助期内，GSR 已将其服务从基因分型扩展到许多其他基因组分析。目前，GSR 提供​​核酸分离和质量分析、基因分型以及基因表达、拷贝数改变和甲基化分析服务，以支持夏威夷大学 (UH) 癌症中心进行的同行评审和 IRB 批准的研究成员。对癌症生物学研究的样本以及流行病学和临床研究中收集的样本进行了各种分析，这些分析利用了多种族人群（尤其是来自夏威夷的多种族人群）的遗传异质性。这些研究的规模从几个到数万个样本不等，重点范围从单个 SNP 或基因到全基因组，对每个样本进行的检测范围从 1 个到超过 100 万个不等。目标是将现有的人类基因组信息转化为有关癌症病因、预防和治疗的新知识。随着几个微阵列平台的增加，GSR 显着扩展了其服务范围，并为夏威夷大学癌症中心和联盟的更多用户（包括基础科学研究人员）提供服务。 GSR 通过提供使用尖端和高吞吐量技术的中央服务来减少中心的裁员。此外，GSR的人员可以在项目执行过程中提供项目规划建议和故障排除能力。 GSR 的人员在分子生物学和遗传学领域拥有丰富的知识，可以为新手和高级用户提供夏威夷其他地方所没有的技术支持、咨询和信息服务。