GENETIC TRANSFORMATION OF MOUSE EMBRYOS

小鼠胚胎的遗传转化

• 批准号: 8172947
• 负责人: Hans Michael Kubisch
• 金额: $ 6.18万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8172947
• 关键词: Animals; Area; Computer Retrieval of Information on Scientific Projects Database; Embryo; Female; Funding; Genetic Transformation; Grant; Housing; Human Chorionic Gonadotropin; Individual; Injection of therapeutic agent; Institution; Litter Size; Location; Longevity; Maintenance; Methods; Mus; Oocytes; Ovum; Production; Research; Research Personnel; Resources; Source; Superovulation; System; Testing; Transgenic Mice; United States National Institutes of Health; design; male

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: While static and ventilated caging systems are both widely utilized in transgenic mouse production facilities, the effects of caging on mouse oocyte and progeny production have not been sufficiently analyzed. This study was designed to compare the oocyte yield of FVB and B6 mice on individual ventilated and static isolated cages. Methods: FVB and B6 mice were maintained in either static or ventilated cages. Females were subjected to superovulation by injections of FHS and hCG and placed with males. Ova were collected 44 hrs later. Results/Discussion: There appeared to be no differences in oocyte yield between FVB and B6 females or caging system. The average oocyte per female in the static vs. ventilated caging system was 18 vs. 21 in FVB and 31 vs. 31 in B6, respectively. We subsequently tested the females' oocyte yield when they were housed in different areas of the ventilated rack (VR). Animals in cages located at the bottom o the VR produced more oocytes than those animals placed at the middle or top of the rack. To further determine whether this location effect could influence the progeny yield, FVB and ICR dams - six groups per strain at two mice per cage, were paired with one male each. The breeder trios were housed at the middle or the bottom of VR, and their litters and litter size were tracked for one year. Dams housed at the bottom of the VR produced significantly more litters than those housed at the middle of the rack, which was mostly due to a longer life span. This information should prove useful for colony management for both transgenic mouse production and general breeder colony maintenance.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 背景：虽然静态和通风笼养系统都广泛应用于转基因小鼠生产设施中，但笼养对小鼠卵母细胞和后代产生的影响尚未得到充分分析。本研究旨在比较单独通风和静态隔离笼中 FVB 和 B6 小鼠的卵母细胞产量。 方法：FVB 和 B6 小鼠饲养在静态或通风笼中。通过注射 FHS 和 hCG 使雌性超数排卵，并将其与雄性放在一起。 44小时后收集卵子。 结果/讨论：FVB 和 B6 雌性或笼养系统之间的卵母细胞产量似乎没有差异。 在静态笼养系统与通风笼养系统中，FVB 中每只雌性的平均卵母细胞数分别为 18 和 21，B6 中每只雌性的平均卵母细胞数分别为 31 和 31。随后，我们测试了将雌性饲养在通风架（VR）的不同区域时的卵母细胞产量。 位于 VR 底部的笼子中的动物比放置在架子中部或顶部的动物产生更多的卵母细胞。 为了进一步确定这种位置效应是否会影响后代产量，将 FVB 和 ICR 母鼠（每笼两只小鼠，每品系 6 组）与每只雄性配对。 这三只种鸡被安置在 VR 的中部或底部，对它们的产仔数和产仔数进行了为期一年的跟踪。 位于 VR 底部的水坝比位于架子中部的水坝产生的垃圾明显更多，这主要是因为水坝的寿命更长。 该信息对于转基因小鼠生产和一般饲养者群体维护的群体管理很有用。