GENETICS AND GENOME BANKING CORE

遗传学和基因组库核心

• 批准号: 7958696
• 负责人: Hans Michael Kubisch
• 金额: $ 5.8万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7958696
• 关键词: Animals; Blood specimen; Breeding; Cell Line; Collection; Computer Retrieval of Information on Scientific Projects Database; DNA; DNA Library; Data; Deformity; Equipment and supply inventories; Fibroblasts; Funding; Generations; Genome; Grant; Individual; Infant; Institution; Methods; Microsatellite Repeats; Paper; Parents; Phenotype; Primates; Research; Research Personnel; Resources; Sampling; Source; Tremor; United States National Institutes of Health; Work; base; cranium; father role; genetic resource; offspring; working group

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: The primary objective of the core is on banking of genetic resources and on generation of microsatellites to assign paternity. Methods: To date we have predominantly concentrated our efforts on the collection of blood samples from animals that are taken during inventory of the breeding corrals. We have collected blood samples from nearly 3520 animals and have blotted these samples onto archival filter paper. We have also extracted DNA from 116 of the collected samples. We have established primary fibroblast cell lines from 6 animals. These have included two homozygous Krabbe infants (HJ76, HR85) as well as two animals with unusual phenotypes (EA32, skull deformity; EC39, tremors) and their dams. The NPRC Genome Banking Working Group decided to establish DNA banks of representative animals at each of the primate centers consisting of 50 unrelated individuals and ten trios each containing both parents and one offspring. Results/Discussion: To date we have collected blood samples from 30 unrelated individual animals and from nine trios. We have generated microsatellites based on a set of markers developed by David Glen Smith at UC Davis. This set consists of 34 satellites arranged into 6 individual panels. To date we have generated satellites from 116 animals and have sent these to Dr. Smith's lab for fragment size analysis. We expect to obtain electropherogram data shortly and will use these to assign paternity. Work on the next group of animals is ongoing.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 中心，不一定是研究者的机构。 简介：该核心的主要目标是储存遗传资源和生成微卫星来指定亲子关系。方法：迄今为止，我们主要集中精力收集动物的血液样本，这些样本是在清点繁殖畜栏时采集的。我们收集了近 3520 只动物的血液样本，并将这些样本吸干到档案滤纸上。我们还从收集的 116 个样本中提取了 DNA。我们已经从 6 只动物中建立了原代成纤维细胞系。其中包括两只纯合子 Krabbe 婴儿（HJ76、HR85）以及两只具有异常表型的动物（EA32，颅骨畸形；EC39，震颤）及其母亲。 NPRC 基因组库工作组决定在每个灵长类动物中心建立代表性动物 DNA 库，其中包括 50 个无关个体和 10 个三人组，每个三人组包含父母和一个后代。结果/讨论：迄今为止，我们已经从 30 只不相关的个​​体动物和 9 只三只动物身上采集了血液样本。我们根据加州大学戴维斯分校的大卫·格伦·史密斯开发的一组标记生成了微卫星。该组由 34 颗卫星组成，排列成 6 个单独的面板。 迄今为止，我们已经从 116 只动物身上生成了卫星，并将它们发送到史密斯博士的实验室进行碎片大小分析。我们预计很快就会获得电泳图数据，并将使用这些数据来确定亲子关系。下一组动物的研究工作正在进行中。