STRUCTURAL MOLECULAR BIOLOGY SMALL ANGLE X-RAY SCATTERING STATION BEAM LINE 4-2

结构分子生物学小角度X射线散射站束线4-2

• 批准号: 8362069
• 负责人: HIROTSUGU TSURUTA
• 金额: $ 8.22万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362069
• 关键词: Biological; Characteristics; Data Collection; Development; Environment; Fiber; Funding; Grant; In Situ; Incidence; Length; Lipids; Membrane Lipids; Molecular Biology; Monitor; National Center for Research Resources; Principal Investigator; Proteins; Radiation; Research; Research Infrastructure; Resources; Roentgen Rays; Sampling; Silicon; Solutions; Source; System; Temperature; Time; United States National Institutes of Health; Virion; X-Ray Crystallography; cost; detector; instrumentation; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The SSRL SMB small-angle x-ray scattering station BL4-2 is dedicated to structural biology studies, providing state-of-the-art experimental facilities for non-crystalline diffraction and scattering studies on proteins, protein assemblies, virus particles, biological fibers and lipid membranes. The in-hutch instrumentation features a variable-distance pin-hole geometry x-ray scattering camera system, which can be configured to accommodate several data collection modes, including solution scattering, single crystal diffraction, lipid/fiber diffraction and gracing-incidence scattering. This system covers the characteristic length scale in the range of 2 A to 2000 A with seven discrete sample-to-detector distances. Advanced x-ray detectors, Rayonix MX225HE CCD and Dectris Pilatus 300K silicon pixel array detectors, are used for static and time-resolved projects, repsectively. The beam line has seen continued development of instrumentation for sample handling, in-situ monitoring of sample quality, and temperature control of sample environments.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 SSRL SMB小角度X射线散射站BL4-2专用于结构生物学研究，为非晶状体衍射和蛋白质，蛋白质组件，病毒颗粒，生物纤维的非晶体衍射和散射研究提供了最新的实验设施和脂质膜。插入仪器具有可变距离孔孔几何X射线散射摄像头系统，可以配置以适应几种数据收集模式，包括溶液散射，单晶衍射，脂质/纤维衍射和散射散射。该系统涵盖了2 a至2000 a范围内的特征长度比例，具有七个离散的样本到检测器距离。先进的X射线检测器，Rayonix MX225HE CCD和Dectris Pilatus 300K硅像素阵列探测器，用于静态和时间分辨的项目，并重复进行。 梁线已经看到了仪器的持续开发，用于样品处理，样品质量的原位监测以及样品环境的温度控制。