MATURATION INTERMEDIATES OF A T=4 VIRUS CAPSID STUDIED BY TIME-RESOLVED X-RAY SC

通过时间分辨 X 射线 SC 研究 T=4 病毒衣壳的成熟中间体

• 批准号: 8362057
• 负责人: HIROTSUGU TSURUTA
• 金额: $ 0.58万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362057
• 关键词: Caliber; Capsid; Electrostatics; Event; Funding; Grant; Kinetics; Manuals; National Center for Research Resources; Principal Investigator; Process; RNA; Radiation; Research; Research Infrastructure; Resolution; Resources; Roentgen Rays; Series; Shapes; Source; Time; Time Study; United States National Institutes of Health; Virus; Virus-like particle; cost; data acquisition; instrumentation; millisecond; structural biology; time resolved data

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Nudaurelia capensis omega virus-like particle, a T=4 virus capsid, undergoes a pH promoted maturation process. Using the fully upgraded instrumentation on SSRL BL4-2 we have begun studying the kinetics of structural change associated with the maturation process. Performing manual mixing, followed by a series of time-resolved data acquisition (each 3 sec exposure), we have recently been able to isolate a structural intermediate at intermediary pH values between neutral (pH 7.6) and pH 5.0, which was used to promote the maturation in our earlier studies. We also observed that two VLPs with different RNA contents show slightly different shape and diameter. We have initiated the time-resolved studies of the entire process of maturation with a millisecond time-resolution in an attempt to examine our hypothesis that the repulsive electrostatic forces among the capsid subunits at the 3-fold and quasi 3-fold axies being the key triggering event in the maturation.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 nudaurelia capensis omega病毒样粒子（t = 4病毒capsid）经历了pH促进的成熟过程。使用SSRL BL4-2上的完全升级的仪器，我们已经开始研究与成熟过程相关的结构变化动力学。 执行手动混合，然后进行一系列时间分辨的数据采集（每3秒暴露），我们最近能够在中性pH值（pH 7.6）和pH 5.0之间隔离一个结构中间体，用于促进我们早期研究的成熟。我们还观察到两个具有不同RNA含量的VLP显示出略有不同的形状和直径。 我们已经开始使用毫秒的时间分辨率开始对整个成熟过程进行的时间分辨研究，以研究我们的假设，即在3倍和准轴上，在3倍和准轴的capsid亚基中排斥的静电力是关键在成熟中触发事件。