Tissue Resource and Molecular Pathology Core

组织资源和分子病理学核心

基本信息

批准号：
8277117
负责人：
WILLIAM E. GRIZZLE
金额：
$ 18.31万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8277117
关键词：
Acceleration African American Agreement Alabama Aliquot Animal Model Animals Area Authorization documentation Autopsy Award Benign Biological Assay Biological Markers Biopsy Biostatistics Core Breast Cell Line Cell model Cells Clinical Clinical Data Clinical Treatment Collaborations Collection Communication Consent Cooperative Human Tissue Network Databases Detection Development Diagnosis Diagnostic Disease Ensure Enzyme-Linked Immunosorbent Assay Equipment and supply inventories Exocrine pancreas Extramural Activities Faculty Fine needle aspiration biopsy Flow Cytometry Free Will Freezing Gene Expression Genes Head Health Insurance Portability and Accountability Act Hepato-Biliary Neoplasm Hepatobiliary Histology Hospitals Human Human Pathology Human Resources Immunoassay Immunoelectron Microscopy Immunohistochemistry In Situ Hybridization Individual Informed Consent Institutional Review Boards Instruction Investigational Therapies Islet Cell Tumor Laboratories Laser Microscopy Lead Lesion Light Link Liquid substance Malignant Neoplasms Malignant neoplasm of pancreas Mass Spectrum Analysis Methods Microdissection Microscopic Minnesota Molecular Molecular Analysis Molecular Profiling Molecular Target Monitor National Cancer Institute Needles Neoplasms Nitrogen Operative Surgical Procedures Other Genetics Outcome Pancreas Pancreatic Adenocarcinoma Paraffin Paraffin Embedding Pathologic Pathologist Pathology Patients Phase Plasma Preparation Procedures Process Proteins Protocols documentation Publishing Quality Control Reagent Recording of previous events Research Research Methodology Research Personnel Research Project Grants Research Support Resources Saliva Sampling Schedule Secondary to Serum Services Signal Transduction Pathway Slide Social Welfare Solid Specimen Staging Standardization Surrogate Endpoint System Technical Expertise Techniques Technology Terminology Testing Time Tissue Banking Tissue Banks Tissue Microarray Tissue Model Tissue Sample Tissues Transfection Transgenic Animals Translational Research Ultrasonography Universities Urine Work Xenograft procedure animal tissue base career chronic pancreatitis clinical Diagnosis cost data management density diagnosis standard effectiveness measure experience human disease human tissue improved member molecular marker molecular pathology neoplastic neoplastic cell novel pancreas xenograft pancreatic neoplasm programs quality assurance repository success sulfated glycoprotein 2 tissue preparation tissue processing tissue resource tumor

项目摘要

PROJECT SUMMARY (See instructions): UAB has extensive experience in collecting, processing, storing, and supplying a wide range of human tissues to support research. Fresh, frozen, and paraffin preparations of tissues can be supplied as well as unstained tissue slides and other histology services. We will expand our current tissue resource services as part of the Pancreatic SPORE and join with our partners at the University of Minnesota (UMN) to establish a seamless tissue resource. We will 1) optimize pancreatic tissue collections so that specimens are snap frozen in liquid nitrogen in less than 15 minutes and the number of pancreatic tissues collected are greatly increased; 2) expand our bank of well characterized pancreatic pre-invasive and invasive neoplastic specimens and matching uninvolved specimens from patients who have consented for their tissues to be used in genetic and other research along with clinical data/ outcome; 3) provide microdissected specimens for sensitive technologies requiring pure samples of pancreatic neoplasia; 4) establish uniform collection procedures with UMN and other SPORES 5) develop tissue matrix arrays of samples of pre-invasive and invasive pancreatic neoplasia to permit the concomitant study of many different specimens; and 5) to receive, store and distribute primary pancreatic xenograft cells from the collaboration with Johns Hopkins. This Core also will provide access to methods of morphological analysis that are difficult for individual projects to support efficiently, including quantitative light microscopic and immunocytochemical interpretation of tissues and cytologic materials and will offer methods to detect gene products within transfected cells and adjacent tissues. Investigators will have access to sophisticated techniques usually available only for human pathology, including multiplex immunoassays, tissue microdissection, special histology services, in situ hybridization, confocal laser microscopy, quantitative cytomorphometric analysis and flow cytometry. The core will enable unified purchase, characterization and utilization of shared sets of reagents; centrally and expertly performed procedures will free investigators from duplication of research methods, allowing more productive work with the available resources and acceleration of experimental timetables.

项目摘要（请参阅说明）： UAB在收集，加工，存储和提供广泛的人类方面具有丰富的经验支持研究的组织。可以提供新鲜，冷冻和石蜡制剂以及未染色的组织幻灯片和其他组织学服务。我们将扩大当前的组织资源服务一部分胰腺孢子，并与明尼苏达大学（UMN）的合作伙伴一起建立一个无缝组织资源。我们将1）优化胰组织收集，以便标本是快照在不到15分钟的时间内冷冻在液氮中，收集的胰组织数量很大增加； 2）扩大我们富有特征的胰腺前侵入和侵入性肿瘤的银行来自同意其组织的患者的标本和匹配的未参考标本用于遗传和其他研究以及临床数据/结果； 3）提供显微解剖标本对于需要胰腺肿瘤的纯样品的敏感技术； 4）建立统一收集使用UMN和其他孢子的程序5）开发侵入前和侵入性样品的组织矩阵阵列侵入性胰腺肿瘤允许对许多不同标本进行伴随研究；和5）从与约翰·霍普金斯（Johns Hopkins）的合作中接收，存储和分发主要的胰腺异种移植细胞。该核心还将提供对形态分析方法的访问，这对于个人来说很难有效支持的项目，包括定量光显微镜和免疫细胞化学解释组织和细胞学材料，并将提供检测转染细胞内基因产物的方法邻近组织。调查人员将可以使用通常仅适用于人类的复杂技术病理学，包括多重免疫测定，组织显微解剖，特殊的组织学服务，原位杂交，共聚焦激光显微镜，定量的细胞形态分析和流式细胞术。这核心将使共享试剂集的统一购买，表征和利用；集中专业执行的程序将使研究人员免于重复研究方法，从而允许更多可用资源和实验时间表加速的生产性工作。