Tissue Resource and Molecular Pathology Core

组织资源和分子病理学核心

基本信息

批准号：
7962145
负责人：
WILLIAM E. GRIZZLE
金额：
$ 19.31万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-01 至 2015-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7962145
关键词：
Acceleration African American Agreement Alabama Aliquot Animal Model Animals Area Authorization documentation Autopsy Award Benign Biological Assay Biological Markers Biopsy Biostatistics Core Breast Cell Line Cells Clinical Clinical Data Clinical Treatment Collaborations Collection Communication Consent Cooperative Human Tissue Network Databases Detection Development Diagnosis Diagnostic Disease Ensure Enzyme-Linked Immunosorbent Assay Equipment and supply inventories Exocrine pancreas Extramural Activities Faculty Fine needle aspiration biopsy Flow Cytometry Free Will Freezing Gene Expression Genes Head Health Insurance Portability and Accountability Act Hepato-Biliary Neoplasm Hepatobiliary Histology Hospitals Human Human Pathology Human Resources Immunoassay Immunoelectron Microscopy Immunohistochemistry In Situ Hybridization Individual Informed Consent Instruction Investigational Therapies Islet Cell Tumor Laboratories Laser Microscopy Lead Lesion Light Link Liquid substance Malignant Neoplasms Malignant neoplasm of pancreas Mass Spectrum Analysis Methods Microdissection Microscopic Minnesota Molecular Molecular Analysis Molecular Profiling Molecular Target Monitor National Cancer Institute Needles Neoplasms Nitrogen Operative Surgical Procedures Other Genetics Outcome Pancreas Pancreatic Adenocarcinoma Paraffin Paraffin Embedding Pathologic Pathologist Pathology Patients Phase Plasma Preparation Procedures Process Proteins Protocols documentation Publishing Quality Control Reagent Recording of previous events Reproduction spores Research Research Ethics Committees Research Methodology Research Personnel Research Project Grants Research Support Resources Saliva Sampling Schedule Secondary to Serum Services Signal Transduction Pathway Slide Social Welfare Solid Specimen Staging Standardization Surrogate Endpoint System Technical Expertise Techniques Technology Terminology Testing Time Tissue Banking Tissue Banks Tissue Microarray Tissue Model Tissue Sample Tissues Transfection Transgenic Animals Translational Research Ultrasonography Universities Urine Work Xenograft procedure animal tissue base career chronic pancreatitis clinical Diagnosis cost data management density diagnosis standard effectiveness measure experience human disease human tissue improved member molecular marker molecular pathology neoplastic neoplastic cell novel pancreas xenograft pancreatic neoplasm programs quality assurance repository success sulfated glycoprotein 2 tissue preparation tissue processing tissue resource tumor

项目摘要

PROJECT SUMMARY (See instructions): UAB has extensive experience in collecting, processing, storing, and supplying a wide range of human tissues to support research. Fresh, frozen, and paraffin preparations of tissues can be supplied as well as unstained tissue slides and other histology services. We will expand our current tissue resource services as part of the Pancreatic SPORE and join with our partners at the University of Minnesota (UMN) to establish a seamless tissue resource. We will 1) optimize pancreatic tissue collections so that specimens are snap frozen in liquid nitrogen in less than 15 minutes and the number of pancreatic tissues collected are greatly increased; 2) expand our bank of well characterized pancreatic pre-invasive and invasive neoplastic specimens and matching uninvolved specimens from patients who have consented for their tissues to be used in genetic and other research along with clinical data/ outcome; 3) provide microdissected specimens for sensitive technologies requiring pure samples of pancreatic neoplasia; 4) establish uniform collection procedures with UMN and other SPORES 5) develop tissue matrix arrays of samples of pre-invasive and invasive pancreatic neoplasia to permit the concomitant study of many different specimens; and 5) to receive, store and distribute primary pancreatic xenograft cells from the collaboration with Johns Hopkins. This Core also will provide access to methods of morphological analysis that are difficult for individual projects to support efficiently, including quantitative light microscopic and immunocytochemical interpretation of tissues and cytologic materials and will offer methods to detect gene products within transfected cells and adjacent tissues. Investigators will have access to sophisticated techniques usually available only for human pathology, including multiplex immunoassays, tissue microdissection, special histology services, in situ hybridization, confocal laser microscopy, quantitative cytomorphometric analysis and flow cytometry. The core will enable unified purchase, characterization and utilization of shared sets of reagents; centrally and expertly performed procedures will free investigators from duplication of research methods, allowing more productive work with the available resources and acceleration of experimental timetables.

项目摘要（参见说明）： UAB 在收集、加工、储存和供应各种人类资源方面拥有丰富的经验。组织来支持研究。可以提供新鲜、冷冻和石蜡的组织制剂以及未染色的组织切片和其他组织学服务。我们将扩大现有的组织资源服务胰腺 SPORE 的一部分，并与我们在明尼苏达大学 (UMN) 的合作伙伴一起建立一个无缝组织资源。我们将 1) 优化胰腺组织采集，以便标本快速采集液氮冷冻不到15分钟，采集的胰腺组织数量大大增加增加； 2) 扩大我们的胰腺浸润前和浸润性肿瘤特征库标本和匹配的未受影响的标本来自同意将其组织用于与临床数据/结果一起用于遗传和其他研究； 3）提供显微解剖标本用于需要胰腺肿瘤纯样本的敏感技术； 4）建立统一采集使用 UMN 和其他 SPORES 进行程序 5) 开发侵入前和侵入后样品的组织基质阵列侵袭性胰腺肿瘤允许同时研究许多不同的标本； 5) 到与约翰霍普金斯大学合作接收、储存和分发原代胰腺异种移植细胞。该核心还将提供对个人来说难以进行的形态分析方法的访问有效支持的项目，包括定量光学显微镜和免疫细胞化学解释组织和细胞学材料，并将提供检测转染细胞内基因产物的方法和邻近组织。研究人员将能够使用通常仅适用于人类的复杂技术病理学，包括多重免疫分析、组织显微切割、特殊组织学服务、原位杂交、共焦激光显微镜、定量细胞形态分析和流式细胞术。这 core 将实现共享试剂组的统一购买、表征和使用；集中和专业执行的程序将使研究人员免于重复研究方法，从而允许更多利用可用资源进行富有成效的工作并加快实验时间表。