Tissue Resources and Molecular Pathology Core

组织资源和分子病理学核心

基本信息

批准号：
7677161
负责人：
WILLIAM E. GRIZZLE
金额：
$ 12.17万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-01 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7677161
关键词：
Acceleration Aliquot Animal Disease Models Animal Model Animals Applications Grants Authorization documentation Autopsy Biological Assay Biological Markers Biometry Cells Characteristics Clinical Clinical Treatment Collaborations Collection Communication Complex Core Facility Data Databases Dermatologic Dermatology Development Diagnosis Diagnostic Diagnostic Services Disease Elements Ensure Enzyme-Linked Immunosorbent Assay Equipment Equipment and supply inventories Extramural Activities Faculty Feasibility Studies Flow Cytometry Freezing Gene Chips Genes Goals Health Insurance Portability and Accountability Act Histology Human Human Pathology Human Resources Immunoassay Immunoelectron Microscopy Immunohistochemistry In Situ Hybridization Individual Informed Consent Investigational Therapies Laboratories Lasers Lesion Light Link Methods Microdissection Microscopic Molecular Analysis Molecular Target Monitor Northern Blotting Operative Surgical Procedures Other Genetics Outcome Paraffin Pathologic Pathologist Pathology Patients Phase Physiology Pilot Projects Preparation Prevention strategy Privacy Procedures Process Proteins Protocols documentation Publishing Quality Control Radical Mastectomy Reagent Recording of previous events Research Research Ethics Committees Research Personnel Research Project Grants Research Support Resources Rodent Sampling Schedule Services Skin Skin Tissue Slide Social Welfare Source Specimen Standardization Surrogate Endpoint System Technical Expertise Techniques Technology Test Result Testing Therapeutic Time Tissue Banking Tissue Banks Tissue Microarray Tissue Model Tissue Sample Tissues Training Transfection Translational Research Work analytical method animal tissue base computerized cost data management design discount disorder control effectiveness measure experience experimental analysis human disease human tissue imaging modality improved in vivo member molecular marker molecular pathology novel programs prospective quality assurance skills skin disorder skin lesion success tissue preparation tissue processing tissue resource

项目摘要

The Tissue Resource and Molecular Pathology Core Facility has extensive experience in collecting, processing, storing/banking, and distributing a wide range of tissues and controls to support the research of the Skin Diseases Research Center (SDRC) members. Fresh, frozen and paraffin preparations of tissues can be supplied as well as unstained tissue slides, tissue matrix arrays, microdissected tissue components, as well as histology services. The Core also can provide investigators with access to methods of morphological and molecular analysis that are difficult for individual projects to support efficiently. Primarily the Core provides light micrscopic and immunocytochemical interpretation of animal and human tissues and cytologic materials including methods to detect gene products within transfected cells and adjacent tissue. Investigators have access to sophisticated techniques usually available only for human pathology, including immunoelectron microscopy and special histology services such as computerized morphometric analysis (CAS200 and BLISS) systems, in situ hybridization, and flow cytometry. Analytical methods available to the SDRC include high throughput ELISA assays and Western, Southern and Northern blotting. Both multiplex RT-Q-PCR and immunoassays are also available. The involvement of dedicated diagnostic pathologists ensures that all assays are performed on optimally collected, processed and diagnosed tissue components. Centrally performed procedures free investigators from duplication of basic work, allowing more productive work with the available resources and acceleration of experimental timetables. The Core works closely with biostatistical personnel so that clinical/demographic information can be linked easily with tissue specimens. The Core will support all projects of the SDRC and its services will be available to all SDRC members. In addition, its costs will be waived for Pilot and Feasibility Studies, as appropriate, for the collection of preliminary data to support grant applications. Depending on demands for services, the current resources requested will permit about a 50% discount below costs of services to SDRC members.

组织资源和分子病理学核心设施在收集、处理、储存/储存和分发各种组织和对照品以支持皮肤研究疾病研究中心（SDRC）成员。可提供新鲜、冷冻和石蜡组织制剂以及未染色的组织切片、组织基质阵列、显微解剖组织成分以及组织学服务。核心还可以为研究人员提供形态学和分子学方法单个项目难以有效支持的分析。主要是核心提供光动物和人体组织以及细胞学材料的显微镜和免疫细胞化学解释包括检测转染细胞和邻近组织内基因产物的方法。调查人员有获得通常仅适用于人类病理学的复杂技术，包括免疫电子学显微镜和特殊组织学服务，例如计算机形态测量分析（CAS200 和 BLISS）系统、原位杂交和流式细胞术。 SDRC 可用的分析方法包括高通量 ELISA 测定以及 Western、Southern 和 Northern 印迹。多重 RT-Q-PCR 和也可进行免疫测定。专门的诊断病理学家的参与确保所有检测对最佳收集、处理和诊断的组织成分进行。集中执行程序使调查人员免于重复基础工作，从而可以利用现有资源进行更有成效的工作资源和加速实验时间表。该核心与生物统计人员密切合作，以便临床/人口统计信息可以轻松地与组织样本联系起来。核心将支持所有 SDRC 的项目及其服务将向所有 SDRC 成员开放。此外，其费用将被免除酌情进行试点和可行性研究，收集初步数据以支持拨款应用程序。根据服务需求，当前请求的资源将允许大约 50% 低于 SDRC 成员服务成本的折扣。