Rapid and On-site Monitoring of Urinary Arsenic Metabolites

尿砷代谢物的快速现场监测

• 批准号: 8432610
• 负责人: BADAWI M DWEIK
• 金额: $ 24.96万
• 依托单位: GINER, INC.
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-19 至 2013-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8432610
• 关键词: Algorithms; Archives; Arsenates; Arsenic; Arsenites; Bangladesh; Biological Markers; Biological Monitoring; Bladder; Cardiovascular Diseases; Cells; Chemicals; Chronic; Complex; Consultations; Detection; Development; Devices; Diagnostic; Disease; Electrodes; Elements; Ensure; Epidemiologic Studies; Epidemiology; Evaluation; Exposure to; Feasibility Studies; Food; Gold; Health; Heavy Metals; Human; Individual; Kidney; Laboratories; Longevity; Lung; Malignant Neoplasms; Measurement; Measures; Medical; Methods; Microelectrodes; Monitor; Mono-S; Nature; Outcome; Phase; Physiologic pulse; Population; Populations at Risk; Procedures; Process; Public Health; Public Health Schools; Reducing Agents; Reproducibility; Research; Risk; Sampling; Signal Transduction; Site; Skin; Small Business Innovation Research Grant; Source; System; Techniques; Technology; Testing; Time; Toxic effect; Urine; Validation; Variant; Work; base; cost; density; design; disorder prevention; drinking water; exposed human population; improved; instrument; monomethylarsonic acid; novel; oxidation; professor; programs; prototype; response; sensor; sound; tool; urinary

DESCRIPTION (provided by applicant): Giner, Inc. proposes a portable, inexpensive, and rapid-response monitor for arsenic speciation in urine. Arsenic species excreted in urine are reflective of human exposure to toxic arsenic, and therefore this sensor will be a valuable biomonitoring tool in medical diagnostics and epidemiological studies by allowing determination of arsenic exposure faster and less expensive than traditional methods. Improved capability of monitoring exposure will have positive public health outcomes, as chronic exposure to arsenic is associated with health problems including cancer and cardiovascular disease. The Phase I program will develop a monitor prototype capable of direct electrochemical detection of arsenic (III) in urine with an advanced electrode substrate. Quantification of other important arsenic species and metabolites (arsenic [V] and mono- and dimethyl arsinate) will be performed by sequential and rapid conversion to arsenic (III) via chemical and electrochemical methods to obtain the total amount of toxic arsenic in urine. The phase I program feasibility studies will include validation of the alpha prototype with real urine samples from epidemiological studies. The phase II program will result in a practical, robust, and highly accurate device capable of on-site analysis. PUBLIC HEALTH RELEVANCE: A portable, inexpensive, and rapid-response monitor for speciation analysis of inorganic arsenic and arsenic metabolites species in urine is proposed. Traditional methods of assessing arsenic exposure are time-intensive and cost-prohibitive, and therefore such low cost onsite monitor will have positive public health outcomes by improving ability to monitor arsenic exposure in at-risk populations.

描述（由申请人提供）：Giner，Inc。提出了用于尿液中砷形成的便携式，廉价且快速响应监测仪。在尿液中排出的砷物种反映了人类接触有毒砷的暴露，因此该传感器将是医学诊断和流行病学研究中有价值的生物监测工具，通过允许比传统方法更快地确定砷的暴露速度，更便宜。提高监测暴露能力将带来积极的公共卫生结果，因为长期暴露于砷与包括癌症和心血管疾病在内的健康问题有关。 I阶段程序将开发一种能够用高级电极底物在尿液中直接电化学检测（III）直接电化学检测的监视器原型。将通过化学和电化学方法进行顺序和快速转化为砷（III），对其他重要的砷物种和代谢产物（砷[V]和单二甲基和二甲基酯）进行定量，以获得尿中有毒毒素的总量。 I阶段计划的可行性研究将包括对流行病学研究中实际尿液样本的α原型验证。 II期计划将产生一种能够实现现场分析的实用，健壮且高度准确的设备。 公共卫生相关性：提出了尿液中无机砷和砷代谢物物种的物种形成分析的便携式，廉价和快速响应监测仪。评估砷暴露的传统方法是耗时且成本良好的，因此，这种低成本的现场监测仪将通过提高监测高危人群中砷暴露的能力而获得积极的公共卫生结果。