STRUCTURAL STUDIES OF FUNCTIONAL RNA

功能性RNA的结构研究

• 批准号: 8170652
• 负责人: SCOTT A STROBEL
• 金额: $ 1.56万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170652
• 关键词: Binding; Catalytic RNA; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Enzymes; Exons; Funding; Gene Expression; Glycine; GpGp; Grant; Institution; Introns; Laboratories; Ligands; Ligation; Pathway interactions; Proteins; RNA; RNA Splicing; Reaction; Reporting; Research; Research Personnel; Resources; Second Messenger Systems; Signal Transduction; Signaling Molecule; Source; Structure; United States National Institutes of Health; Work; aptamer; glucosamine 6-phosphate; second messenger

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The discovery of the RNA self-splicing group I intron provided the first direct evidence that not all enzymes are proteins. Additionally, it has been found that a group of RNAs called riboswitches can regulate gene expression. One focus in our laboratory is the determination of crystal structures of these RNAs. We recently reported crystal structures of several group I intron splicing intermediates in states related to the exon ligation reaction, and are now undertaking structural studies to characterize the complete splicing pathway of this intron by targeting the first step of splicing and post splicing reactions. Additionally, we have also solved the structure of the GlmS ribozyme bound to its native ligand, glucosamine-6-phosphate. We are now working towards the first structures of the GEMM and glycine riboswitches. The glycine riboswitch contains two aptamers that respond cooperativity to changes in glycine concentration. The GEMM riboswitch is the first example of an RNA that is involved in cell signaling, as it binds a second messenger signaling molecule, cyclic bis(3'-5')diguanylic acid (c-diGMP). This project focuses on the group I self-splicing intron along with the GEMM and glycine riboswitches.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 RNA自剪式组I内含子的发现提供了第一个直接证据，表明并非所有酶都是蛋白质。 此外，已经发现一组称为核糖开关的RNA可以调节基因表达。 我们实验室中的一个重点是确定这些RNA的晶体结构。我们最近报道了与外显子连接反应有关的状态中几个I组内含子剪接中间体的晶体结构，现在正在进行结构研究，以靶向剪接和剪接后反应的第一步来表征该内含子的完整剪接途径。此外，我们还解决了与其天然配体葡萄糖胺-6-磷酸盐结合的GLMS核酶的结构。现在，我们正在努力迈向Gemm和甘氨酸核糖开关的第一个结构。甘氨酸核糖开关包含两个适应器，以对甘氨酸浓度的变化做出响应。 Gemm Riboswitch是参与细胞信号传导的RNA的第一个示例，因为它结合了第二个Messenger信号分子，Cyclic Bis（3'-5'）二甘氨酸酸（C-DIGMP）。该项目重点介绍了I组的自剪式内含子以及Gemm和Glycine Riboswitches。