STRUCTURAL STUDIES OF FUNCTIONAL RNA

功能性RNA的结构研究

• 批准号: 8169281
• 负责人: SCOTT A STROBEL
• 金额: $ 1.4万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169281
• 关键词: Binding; Catalytic RNA; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Exons; Funding; Gene Expression; Glycine; GpGp; Grant; Institution; Introns; Laboratories; Ligands; Ligation; Pathway interactions; Proteins; RNA; RNA Splicing; Reaction; Reporting; Research; Research Personnel; Resources; Second Messenger Systems; Signal Transduction; Signaling Molecule; Source; Structure; United States National Institutes of Health; Work; aptamer; glucosamine 6-phosphate; second messenger

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The discovery of the RNA self-splicing group I intron provided the first direct evidence that not all enzymes are proteins. Additionally, it has been found that a group of RNAs called riboswitches can regulate gene expression. One focus in our laboratory is the determination of crystal structures of these RNAs. We recently reported crystal structures of several group I intron splicing intermediates in states related to the exon ligation reaction, and are now undertaking structural studies to characterize the complete splicing pathway of this intron by targeting the first step of splicing and post splicing reactions. Additionally, we have also solved the structure of the GlmS ribozyme bound to its native ligand, glucosamine-6-phosphate. We are now working towards the first structures of the GEMM and glycine riboswitches. The glycine riboswitch contains two aptamers that respond cooperativity to changes in glycine concentration. The GEMM riboswitch is the first example of an RNA that is involved in cell signaling, as it binds a second messenger signaling molecule, cyclic bis(3'-5')diguanylic acid (c-diGMP). This project focuses on the group I self-splicing intron along with the GEMM and glycine riboswitches.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 RNA自剪接第一组内含子的发现提供了第一个直接证据，证明并非所有酶都是蛋白质。此外，还发现一组称为核糖开关的 RNA 可以调节基因表达。 我们实验室的重点之一是确定这些 RNA 的晶体结构。我们最近报道了几种与外显子连接反应相关状态的I组内含子剪接中间体的晶体结构，并且现在正在进行结构研究，以通过针对剪接的第一步和剪接后反应来表征该内含子的完整剪接途径。此外，我们还解析了与其天然配体 6-磷酸氨基葡萄糖结合的 GlmS 核酶的结构。我们现在正在致力于 GEMM 和甘氨酸核糖开关的第一个结构。甘氨酸核糖开关包含两个适体，可对甘氨酸浓度的变化做出协同反应。 GEMM核糖开关是参与细胞信号传导的RNA的第一个例子，因为它结合第二信使信号分子，环状双(3'-5')二鸟苷酸(c-diGMP)。该项目重点关注 I 组自剪接内含子以及 GEMM 和甘氨酸核糖开关。