STRUCTURAL STUDIES OF FUNCTIONAL RNA

功能性RNA的结构研究

• 批准号: 8361655
• 负责人: SCOTT A STROBEL
• 金额: $ 0.37万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361655
• 关键词: Address; Amino Acids; Binding; Carbon; Data; Employee Strikes; Functional RNA; Funding; Gene Expression; Genes; Glycine; Grant; Ligands; Link; National Center for Research Resources; Nature; Phase; Principal Investigator; RNA; Research; Research Infrastructure; Resources; Source; Structure; System; United States National Institutes of Health; Work; aptamer; cost; small molecule; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Riboswitches are RNA domains that can regulate the expression of associated genes through the binding of a small molecule. The glycine riboswitch recognizes the amino acid glycine to upregulate expression of genes that allow glycine to be utilized as an alternative source of carbon. The most striking feature of this riboswitch is the fact that it can recognize glycine in a cooperative nature, allowing the regulated genes to transition from off to fully on with smaller changes in glycine concentration. The riboswitch is composed of two very similar aptamer domains linked by a short conserved region, and previous work in our lab has identified sections that are thought to be involved in cooperativity. To address the question of how cooperativity of ligand recognition is achieved in this RNA system we are working towards solving the crystal structure of the glycine riboswitch. To date, we have collected native data to approximately 3.6A. Our aims going forward focus on obtaining meaningful phase information and higher quality native data.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 核糖开关是 RNA 结构域，可以通过小分子的结合来调节相关基因的表达。甘氨酸核糖开关识别氨基酸甘氨酸，上调基因表达，使甘氨酸能够用作替代碳源。这种核糖开关最显着的特点是它可以以协作的方式识别甘氨酸，从而允许受调节的基因在甘氨酸浓度变化较小的情况下从关闭转变为完全打开。核糖开关由两个非常相似的适体结构域组成，通过一个短的保守区域连接起来，我们实验室之前的工作已经确定了被认为参与协同作用的部分。为了解决这个 RNA 系统中配体识别的协同性如何实现的问题，我们正在努力解决甘氨酸核糖开关的晶体结构。迄今为止，我们已经收集了约 3.6A 的原生数据。我们未来的目标集中于获取有意义的相位信息和更高质量的本地数据。