STRUCTURAL STUDIES OF FUNCTIONAL RNA

功能性RNA的结构研究

• 批准号: 8361655
• 负责人: SCOTT A STROBEL
• 金额: $ 0.37万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361655
• 关键词: Address; Amino Acids; Binding; Carbon; Data; Employee Strikes; Functional RNA; Funding; Gene Expression; Genes; Glycine; Grant; Ligands; Link; National Center for Research Resources; Nature; Phase; Principal Investigator; RNA; Research; Research Infrastructure; Resources; Source; Structure; System; United States National Institutes of Health; Work; aptamer; cost; small molecule; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Riboswitches are RNA domains that can regulate the expression of associated genes through the binding of a small molecule. The glycine riboswitch recognizes the amino acid glycine to upregulate expression of genes that allow glycine to be utilized as an alternative source of carbon. The most striking feature of this riboswitch is the fact that it can recognize glycine in a cooperative nature, allowing the regulated genes to transition from off to fully on with smaller changes in glycine concentration. The riboswitch is composed of two very similar aptamer domains linked by a short conserved region, and previous work in our lab has identified sections that are thought to be involved in cooperativity. To address the question of how cooperativity of ligand recognition is achieved in this RNA system we are working towards solving the crystal structure of the glycine riboswitch. To date, we have collected native data to approximately 3.6A. Our aims going forward focus on obtaining meaningful phase information and higher quality native data.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 核糖开关是RNA结构域，可以通过小分子的结合来调节相关基因的表达。甘氨酸核糖开关识别氨基酸甘氨酸上调基因的表达，从而使甘氨酸可以用作碳的替代来源。这种核糖开关的最引人注目的特征是，它可以识别合作性质的甘氨酸，从而使受调节的基因从OFF过渡到完全变化，而甘氨酸浓度的变化较小。 Riboswitch由两个非常相似的适体域组成，该域由一个短的保守区域连接起来，我们实验室中的先前工作已经确定了被认为与合作性有关的部分。为了解决该RNA系统如何实现配体识别的问题，我们正在努力解决甘氨酸核糖开关的晶体结构。迄今为止，我们已将本机数据收集到约3.6a。我们的目标将集中在获取有意义的阶段信息和更高质量的本地数据上。