Genomics

基因组学

基本信息

批准号：
8038252
负责人：
JANE M CARLTON
金额：
$ 15.65万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-03-01 至 2013-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8038252
关键词：
Animal Model Arts Bioinformatics Biological Assay Biology C. elegans genome Caenorhabditis elegans Cancer Biology Cells Chemistry Chromatin Structure Chromosome abnormality Chromosomes Communities Computational Biology Computing Methodologies Consultations Core Facility Country CpG Islands DNA DNA Microarray Chip DNA Sequence DNA-Protein Interaction Data Analyses Development Double-Stranded RNA Drosophila genome Drosophila genus Educational Activities Environment Epigenetic Process Equipment Experimental Designs Fostering Gene Expression Genes Genetic Predisposition to Disease Genome Genomics Genotype Goals Guidelines Housing Human Human Genome Individual Institutes Laboratories Lead Leadership Libraries Logistics Malignant Neoplasms Methodology Methylation Mission Molecular Molecular Profiling Mus Mutation Nature Nucleic Acids Pathogenesis Pharmaceutical Preparations Policies Post-Transcriptional Regulation Process Publications RNA Interference Research Research Personnel Resolution SNP genotyping Science Screening procedure Services Small Interfering RNA Structure Techniques Time Validation Work base career chromatin protein comparative genomic hybridization data acquisition data sharing falls feeding functional genomics gene function genome-wide human disease member operation protein expression research study small hairpin RNA

项目摘要

Successful unraveling of the nature and functional dynamics of the human genome together with significant advances in computational biology, scientific research of genome-related phenomena have accelerated our understanding of pathogenesis of cancer.and other human diseases. The focus is at fundamental molecular pathomechanisms such as chromosomal abnormalities, genetic susceptibility, and genome structure as well as the epigenetic, transcriptional and post-transcriptional regulation of gene function. In order to realize the goals of genome-oriented research and to make relevant methodologies readily available to individual NYU Cancer Institute researchers as they embark on their research career, it is imperative to develop institutional core facilities that will support such an effort. The NYU Cancer Institute (NYU Cl) under the leadership of Dr. Steven Burakoff has made a significant effort to respond to the challenges of modern functional genomic science by establishing a state-of-the-art Genomics Facility and most recently the RNAi Core Facility. These two core laboratories work in close co-operation to provide NYU Cl investigators with services and expertise in microarray- or quantitative real-time PCR-based profiling of gene and microRNA expression, of protein- DNA interactions in chromatin structure studies, in DMA analysis such as SNP analysis for genotyping, copy number studies, and DNA sequencing for SNP, mutation or CpG-island methylation studies, and finally with high-throughput RNA interference screens.

成功揭示了人类基因组的本质和功能动态，并具有重要意义计算生物学的进步，基因组相关现象的科学研究加速了我们的研究了解癌症和其他人类疾病的发病机制。重点是基础分子病理机制，如染色体异常、遗传易感性和基因组结构作为基因功能的表观遗传、转录和转录后调控。为了实现以基因组为导向的研究目标，并使相关方法可供各个纽约大学使用癌症研究所的研究人员在开始他们的研究生涯时，必须发展制度支持这一努力的核心设施。纽约大学癌症研究所 (NYU Cl) 在 Dr. Steven Burakoff 为应对现代功能基因组的挑战做出了巨大努力通过建立最先进的基因组学设施和最近的 RNAi 核心设施来推动科学发展。这些两个核心实验室密切合作，为纽约大学 Cl 调查人员提供服务和专业知识在基因和 microRNA 表达、蛋白质的微阵列或定量实时 PCR 分析中染色质结构研究中的 DNA 相互作用、DMA 分析（例如用于基因分型的 SNP 分析）、复制数量研究，以及用于 SNP、突变或 CpG 岛甲基化研究的 DNA 测序，最后高通量RNA干扰筛选。