BIOLUMINESCENT ASSAY FOR GENE EXPRESSION ANALYSIS

用于基因表达分析的生物发光测定

基本信息

批准号：
7480704
负责人：
Val V. Golovlev
金额：
$ 36.44万
依托单位：
SCI-TEC, INC.
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-30 至 2010-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7480704
关键词：
Antibiotic Resistance Antiviral Agents Area Biochemical Reaction Biological Biological Assay Biological Models Biological Sciences Bioluminescence Buffers Collaborations Complementary DNA Computer software DNA DNA Microarray Chip DNA Microarray format DNA biosynthesis Detection Development Diphosphates End Point Equipment Foundations Future Gel Gene Expression Genes Genetic Transcription Genomics Genotype Goals HIV-1 Human Individual Kinetics Label Letters Light Literature Liver Luciferases Manufacturer Name Measurement Measures Methodology Mission Monitor National Center for Research Resources National Institute of Diabetes and Digestive and Kidney Diseases National Institute of General Medical Sciences Nucleic Acids Nucleotides Numbers Oligonucleotides Performance Pharmaceutical Preparations Phase Photons Polymerase Polymerase Chain Reaction Progress Reports Protocols documentation PubMed Publications Publishing RNA RNA replication Radioactive Range Reaction Reagent Reporting Research Reverse Transcription Sampling Series Signal Transduction Site Solid Solutions Staphylococcus aureus Sulfate Adenylyltransferase System Techniques Technology Temperature Tennessee Testing Time United States National Institutes of Health Universities Validation Veterinary Medicine Visible Radiation Work base clinical Diagnosis clinical application college commercialization concept cost cost effective design drug discovery high throughput screening inhibitor/antagonist instrument instrumentation luciferin microbial new technology nucleic acid detection polymerization programs research and development research study technology development

项目摘要

DESCRIPTION (provided by applicant): The goal of this project is to develop a new highly sensitive and cost effective RNA Bioluminescent Assay (RT-qBLA) for high-throughput detection and quantification of RNA and DNA in biological samples. The assay implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing methodology for highly sensitive and accurate quantification of RNA and DNA molecules. The proposed assay has unique sensitivity and dynamic range and is able to measure kinetics of RNA and DNA replication in the solution in real time without long and expensive sample amplification of the real time PCR. The RT-qBLA requires less expensive reagents and equipment than real time PCR and DNA microarrays and can be used in a various commercial assays in life sciences research, drug discovery, and clinical diagnosis.

描述（由申请人提供）：该项目的目标是开发一种新的高灵敏度且具有成本效益的RNA生物发光测定法（RT-qBLA），用于生物样品中RNA和DNA的高通量检测和定量。该测定采用与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序方法，可对 RNA 和 DNA 分子进行高灵敏度和准确的定量。所提出的检测方法具有独特的灵敏度和动态范围，能够实时测量溶液中 RNA 和 DNA 复制的动力学，无需长时间且昂贵的实时 PCR 样品扩增。 RT-qBLA 需要比实时 PCR 和 DNA 微阵列更便宜的试剂和设备，可用于生命科学研究、药物发现和临床诊断的各种商业测定。