Bioluminscence Assay for Gene Expression Analysis

用于基因表达分析的生物发光测定

基本信息

批准号：
7219640
负责人：
Val V. Golovlev
金额：
$ 10万
依托单位：
SCI-TEC, INC.
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-30 至 2007-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7219640
关键词：
bioassay bioengineering /biomedical engineering bioluminescence clinical research cost effectiveness gene expression profiling messenger RNA method development microRNAs nucleic acid quantitation /detection polymerase chain reaction pyrophosphates

项目摘要

DESCRIPTION (provided by applicant): Project Summary/Abstract: The goal of this project is to develop a new highly sensitive and cost effective Reverse Transcription Quantitative Bioluminescence Assay (RT-qBLA) for high-throughput detection and quantification of large (mRNA) and small (microRNA) RNAs in diverse biological samples. The RT-qBLA implements the same detection concept known from pyrosequencing, yet expanding pyrosequencing detection methodology for highly sensitive and accurate quantification of RNA molecules for gene expression analysis. The proposed assay can successfully challenge the real time quantitative RT-PCR technique (RT-qPCR) in many applications and can be superior to RT-qPCR particularly for the analysis of small RNA molecules. The RT-qBLA is requiring less expensive reagents and equipment and can be used in a number of commercial assays for applications in life sciences research, drug discovery, clinical diagnosis, environmental analysis, and biodefense. Project Narrative: The goal of this project is to develop a new high performance cost effective bioluminescence assay for gene expression analysis. The proposed assay provides technologically advanced and significantly less expensive alternative for real time polymerase chain reaction technology, which currently dominates in the market. The proposed bioluminescent assay is universal and can be used in various applications in life science research, drug discovery, clinical diagnosis and biodefense in more than 1,800 clinical laboratories and thousands of life science research laboratories in the U.S.

描述（由申请人提供）：项目摘要/摘要：该项目的目标是开发一种新的高灵敏度且具有成本效益的逆转录定量生物发光测定法（RT-qBLA），用于高通量检测和定量大分子（mRNA）和不同生物样品中的小 (microRNA) RNA。 RT-qBLA 实现了与焦磷酸测序相同的检测概念，但扩展了焦磷酸测序检测方法，可对 RNA 分子进行高灵敏度和准确的定量，以进行基因表达分析。所提出的检测方法可以在许多应用中成功挑战实时定量 RT-PCR 技术 (RT-qPCR)，并且优于 RT-qPCR，特别是对于小 RNA 分子的分析。 RT-qBLA 需要较便宜的试剂和设备，可用于生命科学研究、药物发现、临床诊断、环境分析和生物防御等领域的多种商业测定。项目叙述：该项目的目标是开发一种用于基因表达分析的新型高性能、经济高效的生物发光测定方法。所提出的检测方法为目前在市场上占主导地位的实时聚合酶链反应技术提供了技术先进且成本显着降低的替代方案。所提出的生物发光测定具有通用性，可用于美国 1,800 多个临床实验室和数千个生命科学研究实验室的生命科学研究、药物发现、临床诊断和生物防御等各种应用。