MAGNETIC DISK ARRAY DNA ANALYZER

磁盘阵列DNA分析仪

• 批准号: 6342227
• 负责人: Val V. Golovlev
• 金额: $ 11.23万
• 依托单位: SCI-TEC, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-01 至 2002-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6342227
• 关键词: biomedical equipment development; chemical synthesis; magnetic field; microarray technology; nucleic acid hybridization; nucleic acid quantitation /detection; polymerase chain reaction; technology /technique development

The specific aim of this project is to develop Magnetic Disk Array (MDA) technology for ultra-sensitive and inexpensive molecular analysis for cancer research and diagnosis. The major goals of Phase I are to develop and complete a laboratory MDA device, to introduce a technique for tagging biomolecules by magnetic particles, and to demonstrate rapid and reliable DNA detection with the MDA. The approach we propose is to utilize a new method for preparing and detecting magnetic clusters bonded by a DNA molecule to the surface of a disk which is very similar to a conventional computer diskette. With this approach spacial location of a magnetically tagged DNA can be determined by monitoring magnetic properties of the surface and then can be used to identify the DNA sequence following the same approach as was recently introduced by DNA-on-chip technologies. During Phase I, the specific aims are (1) to systematically investigate magnetic tagging materials to be used for the Magnetic Disk Array (2) To develop the MDA device and evaluate the performance of magnetic disk reading equipment. Special effort will be made to achieve ultra-sensitive DNA detection (3) To demonstrate the use of MDA for monitoring DNA hybridization without the need of amplification of targets DNA. PROPOSED COMMERCIAL APPLICATIONS: The potential commercial applications of MDA include mutation screening for cancer and genetic disease diagnosis for hospitals. Due to fast speed analysis, it is very valuable for biomedical research. It also can be used for DNA fingerprinting for forensic applications as well as rapid microbial DNA analysis in field for biological weapon analysis.

该项目的具体目标是开发磁盘阵列 (MDA) 技术，用于癌症研究和诊断的超灵敏且廉价的分子分析。第一阶段的主要目标是开发和完成实验室 MDA 设备，引入磁性粒子标记生物分子的技术，并演示使用 MDA 进行快速可靠的 DNA 检测。我们提出的方法是利用一种新方法来制备和检测由 DNA 分子结合到磁盘表面的磁性簇，该磁盘与传统计算机软盘非常相似。通过这种方法，可以通过监测表面的磁性来确定磁性标记 DNA 的空间位置，然后可以按照与最近通过 DNA 芯片技术引入的相同方法来识别 DNA 序列。第一阶段的具体目标是（1）系统地研究用于磁盘阵列的磁性标签材料（2）开发MDA设备并评估磁盘读取设备的性能。将特别努力实现超灵敏的 DNA 检测 (3) 演示使用 MDA 来监测 DNA 杂交，而不需要扩增目标 DNA。拟议的商业应用：MDA 的潜在商业应用包括癌症突变筛查和医院遗传病诊断。由于分析速度快，对于生物医学研究非常有价值。它还可用于法医应用的 DNA 指纹识别以及生物武器分析领域的快速微生物 DNA 分析。