COMPUTATIONAL IMAGING CORE

计算成像核心

基本信息

批准号：
8172519
负责人：
DALLAS Melvin HYDE
金额：
$ 3.8万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2011-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8172519
关键词：
Animals Archives Calendar Client Color Computer Retrieval of Information on Scientific Projects Database Computer Systems Computer Workstations Computer software Computers Consultations Core Facility Coupled Data Data Collection Data Set Data Storage and Retrieval Databases Development Devices Equilibrium Equipment Faculty Film Fluorescence Frozen Sections Funding Goals Grant Head Hour Housing Image Image Analysis Institution Link Location Microscope Microscopy Microtome - medical device Molecular Monitor Online Systems Paper Paraffin Pathology Plastics Preparation Printing Process Production Publications Records Research Research Personnel Reservations Resolution Resources Sampling Scanning Services Slide Source Staging Students System Training Training Support United States National Institutes of Health Update Vision base cryostat digital imaging fluorescence imaging image archival system innovation instrument instrumentation light microscopy method development technique development

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core facility provides light microscopy, whole slide scanning, stereology, digital imaging, image database development, and consultation services, primarily for the CNPRC, but also for all campus departments on a recharge basis. The goal is to assist faculty, staff, and students with their research needs for qualitative and quantitative imaging applications. This Core assists with the production of publication quality images and offers consultation on experimental approaches for use with its equipment. Additionally, it is engaged in digitizing and correcting archived film images from various sources but primarily those created by the Pathology Service at the CNPRC. The Core also provides a service to annotate and upload fully corrected digital images into the CNPRC image database. Uploaded images are linked in the database to other information for a specific animal and made available to all who have been granted access. Instrumentation housed in the Core is available 24 hours a day, 7 days a week for trained CNPRC users. Consultation, training, and support service, along with access to the equipment for other departments, is available from 8-5 Monday through Friday. The facility houses the following equipment: + A Cryostat for frozen sections + Microtomes for paraffin and plastic sectioning + Sample preparation equipment for plastic processing and embedding + Data storage servers (expandable multi-terabyte volumes) for archiving client, Pathology Unit, and Imaging Core data. These systems provide rapid access to very large (multi-gigabyte) files or data sets that would otherwise require hours to transfer from other locations in the CNPRC. + Web-based calendar for instrument reservations + An Olympus BX61 research microscope with 1 color and 2 B/W cameras, Olympus DSU spinning disk confocal system, computer controlled stage and focusing system, Molecular Devices MetaMorph image capture and analysis software, and Intelligent Imaging Innovations SlideBook software for stereology and image data collection. + A second Olympus BX61 microscope with Intelligent Imaging Innovations SlideBook software for stereology technique development by the Core's staff + A graphics workstation, flatbed scanner, and a high-quality color corrected printer; these are used for publication quality prints, converting film to digital images, image correction, and image archiving. + Document scanners for rapid digitization and OCR conversion of paper records + A Delta Vision Microscopy System and computer workstation for very high resolution multi-dimensional fluorescence imaging with deconvolution + A computer workstation for offline analysis of stereology data (used for method development by the Core staff), and large multidimensional image analysis + A recently updated Visiopharm VIS/CAST Grid Stereology System for quantitative data collection and automated image analysis + Adobe Photoshop, Adobe Illustrator, and Adobe LightRoom software to support all digital imaging efforts of the Core all computers are outfitted with spectrophotometrically color balanced monitors + A fluorescence stereomicroscope outfitted with a high sensitivity color camera coupled to a computer system + A dual-head brightfield stereomicroscope

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。该核心设施可提供光学显微镜，整个幻灯片扫描，立体学，数字成像，图像数据库开发和咨询服务，主要用于CNPRC，也适用于所有校园部门。目的是协助教师，教职员工和学生对定性和定量成像应用的研究需求。该核心有助于生产出版质量图像，并就其设备使用的实验方法进行咨询。此外，它还参与数字化和纠正来自各种来源的存档胶片图像，但主要是由CNPRC的病理服务创建的胶片图像。核心还提供了一项服务，以注释和上传完全校正的数字图像到CNPRC图像数据库中。上传的图像在数据库中链接到其他特定动物的其他信息，并提供给所有被授予访问权限的人。核心中容纳的仪器每周24小时，每周7天，训练有素的CNPRC用户。咨询，培训和支持服务以及对其他部门的设备的访问，可从周一至周五8-5开始。该设施容纳以下设备： +冷冻部分的低温恒温器 +石蜡和塑料截面的微失量 +用于塑料加工和嵌入的样品准备设备 +数据存储服务器（可归档客户端，病理单元和成像核心数据的可扩展的多端金量）。这些系统可快速访问非常大的（多gabyte）文件或数据集，否则这些文件集将需要数小时从CNPRC中的其他位置转移。 +基于Web的仪器预订日历 +具有1颜色和2个b/w相机的Olympus BX61研究显微镜，Olympus DSU旋转磁盘共聚焦系统，计算机控制的舞台和聚焦系统，Molecular Devices Mocular Decesse MetAmorph Image捕获和分析软件以及智能成像创新的Slide Innovations SLIDEBOOK SLIDEBOKEN SLIDEBOOK SLIDEBOOK SLIDEBOOK SLIDEBOOK软件，用于定向和图像数据收集。 +第二个Olympus BX61显微镜，具有智能成像创新的立体学技术开发的智能成像创新软件。 +图形工作站，平板扫描仪和高质量校正的打印机；这些用于出版质量印刷品，将膜转换为数字图像，图像校正和图像归档。 +文档扫描仪快速数字化和OCR转换纸记录 + Delta视觉显微镜系统和计算机工作站，用于高分辨率的多维荧光成像 +用于离线分析的计算机工作站（由核心人员用于方法开发）和大型多维图像分析 +最近更新的Visiopharm Vis/Cast网格立体系统用于定量数据收集和自动图像分析 + Adobe Photoshop，Adobe Illustrator和Adobe Lightroom软件，以支持核心的所有数字成像工作，所有计算机都配备了分光光度计量的平衡显示器 +荧光立体显微镜，配备了高灵敏度颜色相机与计算机系统耦合 +双头Brightfield立体显微镜