MEASUREMENT OF ANALYTES IN WHOLE BLOOD AND SERUM

全血和血清中分析物的测量

• 批准号: 7955852
• 负责人: ISHAN BARMAN
• 金额: $ 1.9万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955852
• 关键词: Algorithms; Biomedical Research; Blood; Clinical; Computer Retrieval of Information on Scientific Projects Database; Detection; Feasibility Studies; Funding; Grant; Institution; Laboratories; Lasers; Measurement; Measures; Patients; Physicians; Preparation; Raman Spectrum Analysis; Reagent; Research; Research Personnel; Resources; Sampling; Serum; Source; Specificity; Testing; Time; United States National Institutes of Health; Variant; Whole Blood; clinically relevant; improved; luminescence; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rapid and reliable measurements of the levels of clinically-relevant blood analytes are invaluable to physicians when making assessments about a patient's treatment options. Current clinical laboratory tests involve sample preparation, the use of expensive reagents, and are time-intensive. Raman spectroscopy is attractive in that it offers both specificity and can measure multiple analytes at once. In the past years, NIR Raman has been proven to be able to detect multiple blood analytes in the millimolar range. This feasibility study seeks to re-evaluate NIR Raman spectroscopy detection limits in the micromolar range which covers a variety of clinically-important blood analytes. To improve the detection limits, technical challenges encountered in the interpretation of Raman spectra- such as turbidity variation across samples and luminescence background in Raman spectra- will be dealt with and novel applications of Chemometrics algorithms will also be explored.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在评估患者的治疗选择时，快速、可靠地测量临床相关血液分析物的水平对于医生来说非常宝贵。 目前的临床实验室测试涉及样品制备、使用昂贵的试剂，并且非常耗时。 拉曼光谱的吸引力在于它既具有特异性，又可以同时测量多种分析物。 在过去的几年中，近红外拉曼已被证明能够检测毫摩尔范围内的多种血液分析物。 该可行性研究旨在重新评估微摩尔范围内的近红外拉曼光谱检测限，涵盖多种临床重要的血液分析物。 为了提高检测限，将解决拉曼光谱解释中遇到的技术挑战，例如样品之间的浊度变化和拉曼光谱中的发光背景，并将探索化学计量学算法的新颖应用。