MEASUREMENT OF ANALYTES IN WHOLE BLOOD AND SERUM

全血和血清中分析物的测量

• 批准号: 8170389
• 负责人: ISHAN BARMAN
• 金额: $ 2.85万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170389
• 关键词: Algorithms; Blood; Clinical; Computer Retrieval of Information on Scientific Projects Database; Detection; Feasibility Studies; Funding; Grant; Institution; Laboratories; Measurement; Measures; Patients; Physicians; Preparation; Raman Spectrum Analysis; Reagent; Research; Research Personnel; Resources; Sampling; Serum; Source; Specificity; Testing; Time; United States National Institutes of Health; Variant; Whole Blood; clinically relevant; improved; luminescence; novel

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Rapid and reliable measurements of the levels of clinically-relevant blood analytes are invaluable to physicians when making assessments about a patient's treatment options. Current clinical laboratory tests involve sample preparation, the use of expensive reagents, and are time-intensive. Raman spectroscopy is attractive in that it offers both specificity and can measure multiple analytes at once. In the past years, NIR Raman has been proven to be able to detect multiple blood analytes in the millimolar range. This feasibility study seeks to re-evaluate NIR Raman spectroscopy detection limits in the micromolar range which covers a variety of clinically-important blood analytes. To improve the detection limits, technical challenges encountered in the interpretation of Raman spectra- such as turbidity variation across samples and luminescence background in Raman spectra- will be dealt with and novel applications of Chemometrics algorithms will also be explored.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在评估患者的治疗方案时，对医生的临床水平相关血液分析物水平的快速和可靠测量对于医生来说是无价的。 当前的临床实验室测试涉及样品制备，使用昂贵的试剂，并且是耗时的。 拉曼光谱学很有吸引力，因为它既具有特异性，又可以一次测量多个分析物。 在过去的几年中，NIR Raman已被证明能够检测到毫米范围内的多个血液分析物。 这项可行性研究旨在在微摩尔范围内重新评估NIR拉曼光谱检测极限，该范围涵盖了多种临床上重要的血液分析物。 为了提高检测极限，将处理拉曼光谱的解释中遇到的技术挑战 - 例如样品之间的浊度变化和拉曼光谱中的发光背景，并将探讨化学计量算法的新应用。