Animal Core

动物核心

基本信息

批准号：
8002615
负责人：
Jennifer C Sullivan
金额：
$ 32.79万
依托单位：
AUGUSTA UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-08-06 至 2015-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8002615
关键词：
Animal Model Animals Breeding Cells Data Duct (organ) structure Endothelin-1 Ensure Excretory function Genotype Goals Health Health Sciences Heart Rate Hypertension Individual Kidney Kidney Diseases Knock-out Knockout Mice Laboratories Measurement Monitor Mus Physical activity Procedures Quality Control Rattus Role Schedule Science Services Sodium Structure System Telemetry Texas Tissues Universities Utah aquaporin-2 base medical schools meetings pressure programs receptor surgery material symposium

Animal Model Animals Breeding Cells Data Duct (organ) structure Endothelin-1 Ensure Excretory function Genotype Goals Health Health Sciences Heart Rate Hypertension Individual Kidney Kidney Diseases Knock-out Knockout Mice Laboratories Measurement Monitor Mus Physical activity Procedures Quality Control Rattus Role Schedule Science Services Sodium Structure System Telemetry Texas Tissues Universities Utah aquaporin-2 base medical schools meetings pressure programs receptor surgery material symposium

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项目摘要

The primary role of Core C (the Animal Core) is to provide the appropriate animals required by the individual projects within the Program Project. The Animal Core will serve three basic support functions. First, the Animal Core will be responsible for maintaining the ETe-receptor deficient rat colony required by Projects 1 and 2, the cell-specific knockout mice required by Projects 3 and 4, and the appropriate control colonies. Second, the Animal Core will be responsible for generating the collecting duct-specific double knockout mice used in Project 3. Third, the Animal Core will provide for the continuous measurement of mean arterial pressure, heart rate and physical activity using the Data Sciences telemetry systems in all required rat and mouse studies in Projects 1, 2, and 3. Aim 1 is to provide the ETe-receptor deficient rats and the collecting duct NOSI knockout mice. This will include setting up the appropriate breeding pairs, collecting tissue and coordinating with Core B for genotyping, and ensuring that a sufficient number of animals are available for the individual projects. These animal models will be provided by the section of the Core located at the Medical College of Georgia. Aim 2 is to provide the collecting duct ET-1, ETA and ETB receptor knockout mice. This will include setting up the appropriate breeding pairs, genotyping, and coordinating with the Medical College of Georgia and the University of Texas Health Science Center to provide sufficient numbers of animals for the individual projects. These mice will be provided by the University of Utah. Aim 3 is to provide the collecting duct N0S1/ET-1 double knockout mice. This will include breeding floxed NOSI, floxed ET-1 and aquaporin-2 (AQP2)-Cre mice. These are being developed for Project 3. Aim 4 is to provide continuous measurement of mean arterial pressure, heart rate and physical activity in mice and rats at the Medical College of Georgia. This will include scheduling, and providing materials, surgery, and daily monitoring throughout the study for the individual projects. The Animal Core has been structured to provide the highest degree of quality control and allow for consistency in animal models across all of the individual projects of the Program Project. This will involve very closely coordinated efforts and sharing of expertise between the groups at the University of Utah and the Medical College of Georgia.

核C（动物核心）的主要作用是提供个人所需的适当动物计划项目中的项目。动物核心将提供三个基本的支持功能。首先，动物核心将负责维持项目1所需的ETE受体不足的大鼠菌落和2，项目3和4所需的细胞特异性敲除小鼠以及适当的控制菌落。其次，动物核心将负责产生收集的导管特异性双基因敲除小鼠在项目3中使用。第三，动物核心将提供平均动脉的连续测量使用数据科学遥测系统的压力，心率和体育活动中的所有必需的大鼠和项目1、2和3中的小鼠研究。目标1是提供ETE受体不足的大鼠和收集 Nosi敲除小鼠管道。这将包括建立适当的育种对，收集组织和与核心B协调进行基因分型，并确保有足够数量的动物可用于各个项目。这些动物模型将由位于佐治亚州医学院。 AIM 2是提供收集管道ET-1，ETA和ETB受体敲除老鼠。这将包括建立适当的育种对，基因分型以及与佐治亚州医学院和德克萨斯大学健康科学中心提供足够的数字单个项目的动物。这些小鼠将由犹他大学提供。目标3是提供收集管道N0S1/ET-1双基因敲除小鼠。这将包括繁殖floxed nosi，floxed ET-1和Aquaporin-2（AQP2）-CRE小鼠。这些是针对项目3开发的。目标4是为了提供连续测量小鼠和大鼠平均动脉压，心率和身体活动佐治亚州医学院。这将包括调度，提供材料，手术以及每日在整个研究中为各个项目进行监视。动物核心已结构为提供质量控制的最高程度，并允许所有个体的动物模型保持一致性计划项目的项目。这将涉及非常紧密的协调努力和分享专业知识在犹他大学和佐治亚州医学院的团体之间。