Enhancing Dietary Iron and Zinc Bioavailability in Indian Children

提高印度儿童膳食铁和锌的生物利用度

• 批准号: 7665615
• 负责人: STEVEN A ABRAMS
• 金额: $ 6.85万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7665615
• 关键词: Adolescent; Affect; Age; Ascorbic Acid; Bioavailable; Biological Availability; Blood specimen; Calcium; Child; Collaborations; Consumption; Crossover Design; Data; Developed Countries; Developing Countries; Diet; Diet Modification; Dietary Factors; Dietary Intervention; Dietary Iron; Dose; Drug Formulations; Evaluation; Fruit; Gender; Growth; Health; Hemoglobin; India; Institutes; Intake; Iron; Iron deficiency anemia; Label; Laboratories; Lead; Measures; Medicine; Methods; Micronutrients; Minerals; Modification; Nutritional; Oral; Outcome; Participant; Performance at work; Population; Populations at Risk; Process; Protocols documentation; Public Health; Questionnaires; Randomized; Recommended Dietary Allowance; Relative (related person); Risk; Schools; Sensory; Urine; Vegetables; Zinc; Zinc deficiency; absorption; age group; aged; base; boys; college; cost; dietary mineral; feeding; fruits and vegetables; girls; hedonic; improved; inhibitor/antagonist; nutrition; population based; prevent; programs; public health relevance; sound; stable isotope

DESCRIPTION (provided by applicant): Background: Iron and zinc deficiencies frequently occur together because the dietary factors that impair iron absorption also affect zinc absorption. There is no recommended daily intake for zinc in India. With stable isotopes of iron and zinc it is now possible to measure precisely iron and zinc absorption. The specific objectives are (1) To measure the bioavailability of iron and zinc from a representative Indian diet (2) To measure the change in bioavailability of iron and zinc achievable by feasible diversification of a representative Indian diet (3) to determine the tolerability of the feasible dietary modifications. Methods: The proposed program uses a cross-over design to study healthy children aged 13-15.9 years (16 boys and 16 girls) selected from residential schools. Baseline iron and zinc status will be assessed. All children will be randomly assigned to receive either the representative diet or the diversified diet. The proposed modification is the inclusion of ~ 20 mg ascorbic acid from fruits and vegetables which will be served with each meal. Children will be fed their respective diets for 5 consecutive days labeled with 57Fe (for 3 days only, two main meals) with an oral reference dose of 58Fe (with ascorbic acid) on day 4. Blood samples will be collected at baseline and on day 18. Simultaneously 67Zn will be given with the first meal and a reference dose of 70Zn injected intravenously on the same day. Urine will be collected from day 3 to day 6. Absorption will be quantitated by the relative enrichment of 57Fe and 58Fe in hemoglobin for iron or by 67Zn and 70Zn in urine for zinc using ICP-MS. The participants will then crossover to the other diet on day 19, following the same protocol. Tolerability of the diversified diet will be assessed for 6 weeks by administering a sensory evaluation questionnaire to the participants using a 5-point hedonic scale. Outcomes: This project will generate data on the absorption of iron and zinc in adolescents to provide a basis for determining the RDA's and the feasibility of dietary modification as a strategy to prevent iron and zinc deficiencies. The establishment of a long term collaboration between the stable isotope laboratories at Baylor College of Medicine and the National Institute of Nutrition to evaluate nutritional problems among children in India will be an important outcome of this project. PUBLIC HEALTH RELEVANCE: This study will provide evidence that achievable dietary changes in a very "at-risk" group, adolescents, in India can have important benefits in improving the amount of iron and zinc absorbed from the diet. Improving the intake of bioavailable minerals would be crucial to enhancing the health of the Indian population as it will improve school and work performance in both boys and girls. These benefits will lead to larger population-based studies and an enhanced understanding of using nutritional interventions in developing countries to improve health.

描述（由申请人提供）：背景：铁和锌不足经常同时发生，因为损害铁吸收的饮食因素也会影响锌的吸收。印度锌的锌每日摄入均不建议。借助铁和锌的稳定同位素，现在可以精确地测量铁和锌的吸收。特定目标是（1）测量来自代表性的印度饮食（2）的铁和锌的生物利用度，以衡量通过可行的印度饮食可行多样化来确定可行饮食可行修饰的可耐受性，可以实现铁和锌的生物利用度的变化。方法：拟议的计划使用跨界设计来研究从住宅学校中选择的13-15.9岁（16名男孩和16个女孩）的健康儿童。将评估基线铁和锌状态。所有儿童将被随机分配接受代表性饮食或多样化的饮食。拟议的修饰是从水果和蔬菜中含有约20毫克的抗坏血酸。儿童将连续5天被喂食，并在第4天用57FE（仅3天，两天，两顿主餐）和口服参考剂量为58fe（抗坏血酸）。在碱基和第18天将收集血液样本。同时在第一顿餐食中同时给予67zn的第一顿餐点，并使用70zn Intrave in Intrave in Intrave Indrave Indrave in Intrave in Indrave in Indrave Indrave Indrave。尿液将从第3天到第6天收集。使用ICP-MS的57FE和58FE血红蛋白的相对富集或58FE的血红蛋白或67zn和70zn的尿液来定量吸收。然后，参与者将按照相同的协议在第19天的其他饮食进行跨越。多元化饮食的耐受性将通过使用5分享乐量表对参与者进行感官评估问卷来评估6周。结果：该项目将生成有关青少年铁和锌吸收的数据，以提供确定RDA的基础，以及饮食修饰的可行性，作为防止铁和锌缺陷的策略。贝勒医学院的稳定同位素实验室与国家营养研究所评估印度儿童营养问题之间的长期合作将是该项目的重要结果。 公共卫生相关性：这项研究将提供证据表明，印度非常“处于危险”的群体中可实现的饮食变化可以在改善饮食中吸收的铁和锌的含量方面具有重要好处。改善生物利用矿物质的摄入量对于提高印度人口的健康至关重要，因为这将改善男孩和女孩的学校和工作表现。这些好处将导致更大的基于人群的研究，并增强对使用发展中国家的营养干预措施改善健康的理解。