CSHL Course on Proteomics

CSHL 蛋白质组学课程

基本信息

批准号：
7292724
负责人：
DAVID J. STEWART
金额：
$ 7.42万
依托单位：
COLD SPRING HARBOR LABORATORY
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7292724
关键词：
Academia Address American Brain Tumor Association Animals Area Attention Automobile Driving Bacteriology Bacteriophages Base Sequence Basic Science Behavioral Biochemical Biochemistry Bioinformatics Biological Biological Markers Biological Process Biological Sciences Biology Boston Botany Brain Neoplasms Brazil C. elegans genome Caenorhabditis elegans Cell membrane Cells Cessation of life Chemicals Clinical Cloning Cods Collaborations Complement Complex Computational Biology Computer software Computers Coupling Crystallography DNA DNA Microarray Chip DNA Microarray format DNA Sequence Data Data Set Depth Detection Developmental Cell Biology Discipline Disease Drosophila genus Drug Delivery Systems Early Diagnosis Ectopic Expression Educational Curriculum Educational process of instructing Educational workshop Embryology Emerging Technologies Ensure Etiology Eukaryota Eukaryotic Cell Exposure to Faculty Foundations Funding Gene Expression Genes Genetic Genetic Recombination Genetic Transcription Genome Genomics Goals Grant Hand Human Human Genome Image Immersion Investigative Technique In Situ Hybridization Individual Industry Informatics Institutes Ion Channel Journals Knowledge Laboratories Lead Learning Life Location Long Island Malignant Neoplasms Malignant neoplasm of brain Mass Spectrum Analysis Massachusetts Medical Mentors Methodology Methods Michigan Microarray Analysis Microbial Genetics Microbiology Microscope Modeling Modification Molecular Molecular Biology Molecular Cloning Mus Names National Cancer Institute National Institute of Child Health and Human Development National Institute of Mental Health Nature Nervous system structure Neurobiology Neurosciences Normal Cell Nucleotides Numbers Organism Participant Pattern Peptides Personal Communication Phage Display Phenotype Philosophy Physiological Physiology Plants Play Policies Polymerase Chain Reaction Positioning Attribute Post-Translational Protein Processing Postdoctoral Fellow Preparation Principal Investigator Process Progress Reports Prokaryotic Cells Property Protein Analysis Protein Complex Subunit Protein Interaction Mapping Protein Microchips Protein-Protein Interaction Map Proteins Proteolysis Proteome Proteomics Protocols documentation Publications RNA Splicing Recording of previous events Relative (related person)Research Research Institute Research Personnel Research Training Robotics Roentgen Rays Role Sampling Schools Science Scientist Series Serum Signal Pathway Slide Stem cells Structure Students Surface System Techniques Technology Time Tissues Training Transcript Translations Tumor Tissue Two-Dimensional Gel Electrophoresis Universities Update Variant Viral Genes Virus Cultivation Vision Week Wood material Work Writing Xenopus Zoology bacterial genetics base biological research cell behavior cell type cellular imaging comparative computational neuroscience concept day density experience expression vector falls functional genomics genome sequencing human disease immunocytochemistry instructor interest lecturer lectures mRNA Differential Displays miniaturize neoplastic cell neuromechanism novel polypeptide professor programs protein expression protein protein interaction protein purification rat genome relating to nervous system research study size statistics stem structural biology tool trend tumor yeast genetics

项目摘要

DESCRIPTION (provided by applicant): The proposed Cold Spring Harbor Laboratory Course on Proteomics is to be held July 7-20, 2006, 2007, 2008, 2009 and 2010. This intensive laboratory and lecture course will focus on two major themes in proteomics. In the profiling section of the course, students will learn methodologies of protein preparation from diverse samples, sample analysis by 2-D gel electrophoresis and mass spectrometry, and the application of bioinformatics tools to identify proteins and assess their relative abundance. In the functional proteomics section of the course, students will learn the use of recombinational cloning to move many genes simultaneously to different expression vectors, how to apply robotics to high-throughput methods, and how to perform high-throughput expression, purification and characterization of proteins. The overall aim of the course is to provide each student with the fund of knowledge and hands-on experience necessary to be able to perform and analyze proteomics experiments, and to learn to identify new opportunities in applying proteomics approaches to his/her own research.

描述（由申请人提供）：拟定的冷泉港蛋白质组学实验室课程将于 2006 年、2007 年、2008 年、2009 年和 2010 年 7 月 7 日至 20 日举行。这一强化实验室和讲座课程将重点关注蛋白质组学的两个主要主题。在课程的分析部分，学生将学习从不同样品中制备蛋白质的方法、通过二维凝胶电泳和质谱进行样品分析，以及应用生物信息学工具来识别蛋白质并评估其相对丰度。在课程的功能蛋白质组学部分，学生将学习如何使用重组克隆将许多基因同时转移到不同的表达载体，如何将机器人技术应用于高通量方法，以及如何进行高通量表达、纯化和表征蛋白质。该课程的总体目标是为每个学生提供必要的知识和实践经验，以便能够进行和分析蛋白质组学实验，并学会识别将蛋白质组学方法应用于自己的研究的新机会。