Core C: Structural Imaging Core

核心 C：结构成像核心

• 批准号: 7751476
• 负责人: SIMON C WATKINS
• 金额: $ 15.15万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7751476
• 关键词: Achievement; Algorithms; Animals; Antigens; Arts; B-Lymphocytes; Brightfield Microscopy; Cell Communication; Cells; Collection; Color; Competence; Computer Assisted; Computers; Data; Data Set; Databases; Dendritic Cells; Devices; Dimensions; Electron Microscope; Electron Microscopy; Electrons; Ensure; Equipment; Evaluation; Event; Experimental Designs; Fluorescence; Fluorescence Microscopy; Fluorochrome; Four-dimensional; Freeze Fracturing; Frozen Sections; Functional disorder; Grant; Hemorrhagic Shock; Home environment; Housing; Image; Image Analysis; Imagery; Imaging Device; Imaging technology; Immunoelectron Microscopy; In Situ; Inflammatory; Injection of therapeutic agent; Injury; Instruction; Label; Laser Scanning Microscopy; Lasers; Life; Light; Liver; Lung; Measurement; Medical center; Messenger RNA; Methods; Microscope; Microscopic; Microscopic Autoradiography; Microscopy; Microtomy; Modeling; Molecular; Molecular Biology; Optical Methods; Optics; Organ; Pathology; Pathology processes; Penetration; Phase-Contrast Microscopy; Photons; Principal Investigator; Process; Proteins; Publishing; Research; Research Design; Research Personnel; Resolution; Scanning; Scanning Transmission Electron Microscopy Procedures; Services; Shock; Spatial Distribution; Specimen; Speed; Staining method; Stains; System; Techniques; Technology; Time; Tissues; Training; Trauma; Universities; Work; cellular imaging; charge coupled device camera; computerized; design; digital; digital imaging; experience; fluorescence imaging; image processing; instrument; instrumentation; interest; islet; light microscopy; member; morphometry; optical imaging; programs; research study; tool; transmission process

The central theme of this proposal is to dissect the molecular mechanisms leading to tissue injury, and organ dysfunction in trauma. Visualization and localization of message, protein, or structural changes leading to, or resulting from each step in this dynamic process is essential in understanding the molecular mechanisms of trauma. The different projects within this proposal focus on defined tissue systems including gut, liver and lung within models of shock and or trauma. In each case a wide range of microscopic methods will be employed and are considered essential in gaining an understanding the pathology of HS at the tissue and cellular level, thus a central cell and tissue imaging core is defined as an integral component of this proposal. The Core will be housed in the Center for Biologic Imaging (CBl) of the University of Pittsburgh Medical Center. This Center is equipped to perform a continuum of optical methods including all types of microscopy essential to this Program Project. Within the scope of this Program light microscopic techniques include: histological, immuno-histological, laser confocal, 2 photon, evanescent wave, live cell and whole dssue/animal imaging technologies. Our considerable experience in computerized image processing and morphometry will allow quantitative analysis of observed phenomena to corroborate subtle qualitative changes, and this a major function of the Core in this Program. At the electron microscopic level thin section electron microscopy and immuno-electron microscopic evaluation of specimens as a natural extension of the light microscopic analyses will be employed when needed. During the previous grant cycle the CBl collaborated extensively with all of project leaders, as is described in the preliminary data section and we expect a continued expansion in the use of optical techniques. Furthermore we have developed and built multiple new instruments to facilitate these interactions at all levels from the single cell to the whole animal RELEVANCE (See Instructions): Optical imaging is an essential core function within the program providing quantitative image based data for subsequent analysis using computer aided tools also available within the core

该提案的中心主题是剖析导致组织损伤的分子机制，器官 创伤功能障碍。消息，蛋白质或结构变化的可视化和定位导致或 在这种动态过程中，每个步骤产生的步骤对于理解分子机制至关重要 创伤。该提案中的不同项目集中于肠道，肝脏和肺在内的定义的组织系统 在冲击和 /或创伤的模型中。在每种情况下 被认为在了解组织和细胞水平上HS的病理时被认为至关重要，因此 中央细胞和组织成像核心被定义为该提案的组成部分。核心将被安置 在匹兹堡大学医学中心的生物成像中心（CBL）中。这个中心配备了 执行连续的光学方法，包括该程序项目必不可少的所有类型的显微镜。 在此程序范围内，光显微镜技术包括：组织学，免疫 - 历史学，激光 共聚焦，2个光子，evanescent波，活细胞和整个DSSUE/动物成像技术。我们的大量 计算机图像处理和形态计量学的经验将允许对观察到的定量分析 证实微妙的定性变化的现象，这是该程序中核心的主要功能。在 电子显微镜水平薄截面电子显微镜和免疫电子显微镜评估 在需要时，将采用样品作为光显微镜分析的自然扩展。在 以前的赠款周期CBL与所有项目负责人进行了广泛的合作，如 初步数据部分，我们预计使用光学技术会继续扩展。此外，我们 已经开发并构建了多种新仪器，以促进单个级别的各个级别的相互作用 整个动物的细胞 相关性（请参阅说明）： 光学成像是该程序中必不可少的核心功能，为基于定量图像的数据提供 随后使用计算机辅助工具的分析也可以在核心内使用