Quantitative Analysis and Mapping of Metals in Tissues

组织中金属的定量分析和绘图

• 批准号: 7502716
• 负责人: ZEWU CHEN
• 金额: $ 54.73万
• 依托单位: X-RAY OPTICAL SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7502716
• 关键词: Ablation; Agriculture; Agrochemicals; Alzheimer' s Disease; Amyotrophic Lateral Sclerosis; Anodes; Area; Brain; Cessation of life; Clinic; Clinical; Clinical Research; Complex; Computers; Coupled; Data; Deposition; Electronics; Electrons; Ensure; Fluorescence; Forensic Medicine; Goals; Heart; Hepatolenticular Degeneration; Human; Huntington Disease; Hybrids; Ions; Kidney; Knowledge; Laboratories; Laboratory Personnel; Laboratory Research; Liver; Magnetic Resonance; Magnetic Resonance Imaging; Maps; Mass Spectrum Analysis; Measurement; Medical; Metals; Microscopic; Motion; Mus; Neurodegenerative Disorders; Neurofibrillary Tangles; Optics; Pathology; Patients; Pharmacologic Substance; Phase; Pilot Projects; Plasma; Power Sources; Preparation; Reporting; Research; Research Subjects; Resolution; Roentgen Rays; Role; Sampling; Scanning; Sectioning technique; Senile Plaques; Societies; Source; Spatial Distribution; Spectrometry, Mass, Secondary Ion; Staging; Staining method; Stains; Synchrotrons; System; Techniques; Technology; Testing; Time; Tissues; Trace Elements; Trace metal; Training; Tungsten; United States National Institutes of Health; Vertebrates; base; bone; bone lead; brain tissue; college; commercialization; design; detector; human tissue; improved; innovation; instrument; laser spectrometry; prototype; social

DESCRIPTION (provided by applicant): The objectives of this Phase II project are to develop and demonstrate a monochromatic-micro-x-ray fluorescence (M¿XRF) instrument for high sensitivity and high spatial resolution (10-20 ¿m) mapping of trace metal distributions in human brain tissue from deceased Alzheimer's research subjects. After scanning, the brain tissue sections will be stained and microscopically examined for A (beta amyloid) plaque, neurofibrillary tangles and other cellular abnormalities to examine correlations. Whole brain magnetic resonance (MRI) measurements on the research subject during extended pre-death treatment and study and post-mortem whole brain and micro MRI measurements of related tissue sections. This will comprise the most comprehensive correlated metal-neurohistological study of Alzheimer's disease (AD) to date. The same instrument can also be used to study elemental distributions in other medical materials (e.g. heart, liver, kidney, bone, etc.), and environmental, agricultural, industrial, forensic, scientific or other materials, often with no special sample preparation. Current elemental scanning technologies are limited in sensitivity (conventional micro x- ray fluorescence, electron microbeam), expensive and/or inaccessible (Synchrotron micro x-ray fluorescence), or destructive and complex (focused ion beam secondary ion mass spectrometry and laser ablation inductively coupled plasma mass spectrometry). The compact, low power, affordable trace element mapping instrument is based on new, powerful, proprietary, monochromatic doubly curved crystal (DCC) x-ray optics technology developed by X-Ray Optical Systems, Inc., East Greenbush, NY. The resulting analyzer is a safe, reliable, compact, easy to operate, scanning instrument for use in the research clinic for high resolution (10-20 ¿m) elemental spatial distribution measurements. The benefit to society will be improved knowledge of the role of metal deposits which characterize more than 20 defined neurodegenerative diseases (AD, PD, ALS, MS, Wilson's disease, Huntington's disease, etc.). Use in bone lead, renal, heart, liver, etc. studies of other medical pathologies and in other fields such as forensic, atmospheric, geological, agricultural, chemical, pharmaceutical etc. will, over time, greatly broaden the medical, scientific, industrial and social benefits. Tissue mapping proposal narrative The compact, low power, affordable trace element mapping instrument to be developed in this project is based on new, proprietary, x-ray optics technology developed by X-Ray Optical Systems, Inc., East Greenbush, NY. The resulting analyzer is a safe, affordable, reliable, compact, easy to operate, scanning instrument for high resolution (10-20 ¿m) elemental distribution measurements in brain tissue sections and will give improved knowledge of the role of brain metal deposits which accompany virtually every identified neurodegenerative disease (AD, PD, ALS, MS, Wilson's disease, Huntington's disease, etc.). Similar use in bone lead, renal, heart, liver, and other medical. studies and in other scientific, and industrial applications show potential for important societal benefit.

描述（由适用提供）：该II期项目的目标是开发和证明单色微X射线荧光（M€XRF）仪器，用于高灵敏度和高空间分辨率（10-20 m）从已故的阿尔茨海默氏症研究对象中的人脑组织中痕量金属分布的高空间分辨率（10-20 m）映射。扫描后，将对（β-淀粉样蛋白）斑块，神经原纤维缠结和其他细胞异常进行检查，对脑组织切片进行染色和显微镜检查。在延长的死亡前治疗和研究后，全脑磁共振（MRI）测量以及尸体后全脑和相关组织切片的微MRI测量值。迄今为止，这将完成对阿尔茨海默氏病（AD）的最全面相关的金属神经组织学研究。该仪器还可以用于研究其他医疗材料（例如心脏，肝脏，肾脏，骨骼等）以及环境，农业，工业，法医，科学或其他材料的元素分布，通常没有特殊的样品制备。当前的元素扫描技术的灵敏度（常规微X射线荧光，电子微束），昂贵和/或无法访问（同步X射线荧光）或破坏性和复杂性（聚焦离子束次要离子质量光谱仪和激光元素均可量度均可群质量质量群体群体群体群体群体群体群体群体群体群体群体群体群体群体群体，该质量质量群落质量群体，基于X射线光学系统开发的新型，强大的，专有的双重曲面（DCC）X射线光学技术，纽约州East Greenbush。具有20多个定义的神经退行性疾病的金属沉积物（AD，PD，ALS，MS，Wilson氏病，亨廷顿氏病等）。组织映射提案叙述本项目中要开发的紧凑，低功率，负担得起的微量元素映射工具基于X射线光学系统，纽约州East Greenbush开发的新的，专有的，专有的X射线光学技术。最终的分析仪是一种安全，负担得起，可靠，紧凑，易于操作的，扫描工具，用于高分辨率（10-20�M）元素分布在脑组织段中的分布测量，并将提高人们对脑金属沉积物的作用，这些脑金属沉积物的作用可容纳几乎所有已鉴定的神经退行性疾病（AD，PD，PD，ALS，ALS，MS，MS，WILSON病，Wilson的病），等等。在骨骼铅，肾脏，心脏，肝脏和其他医学中的类似用途。研究和其他科学和工业应用显示了重要的社会益处的潜力。