Oxidation State Determination in Chromium Carcinogenesis

铬致癌过程中氧化态的测定

• 批准号: 6605848
• 负责人: ZEWU CHEN
• 金额: $ 46.59万
• 依托单位: X-RAY OPTICAL SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6605848
• 关键词: X ray spectrometry; bioimaging /biomedical imaging; chemical carcinogenesis; chromium; intracellular; optics; oxidation; stable isotope; technology /technique development; tissue /cell culture

DESCRIPTION (provided by applicant): Some chromium (VI) compounds are documented human propose measuring the intracellular oxidation state of Cr carcinogens, but the exact mechanism of Cr induced cancer is unknown. Oxidation state determination of intracellular Cr will provide important information relevant to exposure and risk assessment, and for understanding the mechanism of Cr carcinogenesis. We propose measuring the intracellular oxidation state of Cr using x-ray absorption near edge structure (XANES) spectroscopy. Until now, the XANES technique was not very practical, relying on costly synchrotrons. This valuable technique could be implemented using a lower power cost-effective system incorporating a revolutionary toroidal crystal optic. XOS has successfully carried out the Phase I research and demonstrated the feasibility of Cr oxidation determination using a table-top XANES system operated with samples in air. In this Phase II project, a fully optimized system will be designed. Human cells will be prepared in culture mediums and treated with Cr exposure. The reduction of Cr(Vl) to Cr(lll) will be monitored using the XANES system. The practical application of this XANES technique for clinical research and its commercialization will be evaluated. This would make available, for the first time, a broad chemical-state measurement capability covering most of the periodic table in a compact, low-power table-top system for medical, research, and industrial applications.

描述（由申请人提供）：某些铬（VI）化合物被记录在人类提出的测量Cr致癌物的细胞内氧化态，但是CR诱导癌症的确切机制尚不清楚。细胞内CR的氧化状态确定将提供与暴露和风险评估有关的重要信息，并了解CR癌变的机制。我们建议使用X射线吸收在边缘结构（XANES）光谱法测量Cr的细胞内氧化态。到目前为止，Xanes技术依赖于昂贵的同步性。这种有价值的技术可以使用较低的功率具有成本效益的系统来实施，该系统结合了革命性的环形晶体光学元件。 XOS已成功进行了I期研究，并使用了空气中的样品运行的台式XANES系统证明了CR氧化测定的可行性。在此II阶段项目中，将设计一个完全优化的系统。人类细胞将在培养基中制备，并通过CR暴露处理。将使用XANES系统监测CR（VL）对CR（LLL）的降低。该XANES技术在临床研究及其商业化中的实际应用将得到评估。这将首次提供广泛的化学状态测量能力，涵盖了大多数元素周期表，以用于医疗，研究和工业应用的紧凑，低功能台式系统。