Defining the role of the ABI4 transcription factor in the sugar regulated control of storage oil breakdown in Arabidopsis

定义 ABI4 转录因子在拟南芥贮藏油分解糖调节控制中的作用

• 批准号: BB/E022081/1
• 负责人: Ian Graham
• 金额: $ 56.23万
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE022081%2F1
• 关键词: Defining; role; ABI4; transcription; factor

All living organisms maintain constant levels of soluble carbohydrates in their cells, and use these to support respiration and growth. In order to achieve this carbohydrate concentration is sensed and appropriate metabolic pathways are activated and repressed to increase or decrease the flow of carbon through essential processes as required. This process is known as metabolic feedback. Failure to regulate carbohydrate levels can lead to slow development or even disease, such as in the case of diabetes. In plants this process regulates the accumulation of stored reserves, which form much of the food we eat. Plants sense the intracellular concentration of soluble sugars and in times of excess up-regulate reserve deposition, such as starch synthesis, and in times of depletion up-regulate reserve breakdown, either from starch, fatty acids or amino acids from proteins. During early seedling development plants are dependent on the breakdown of stored fatty acids for their energy source, as they have not yet developed the apparatus for fixing carbon from the atmosphere through photosynthesis. Fatty acid catabolism can be inhibited by the provision of sugars, making this an excellent model system to study metabolic feedback networks in plants. We have recently shown that the ABI4 gene represses lipid breakdown in the model plant Arabidopsis. ABI4 encodes a transcription factor, meaning that it's function is to control the activity of other genes. Many of the known target genes of ABI4 are subject to regulation by metabolic status, suggesting a role in metabolic feedback control. ABI4 also functions in the response to the plant hormone ABA which is well known to play a role in plant sugar responses. This work aims to understand how metabolic feedback control works in plants, how ABI4 action is regulated and how ABI4 can turn off fatty acid catabolism in response to sugar levels.

所有生物体都保持其细胞中恒定水平的可溶性碳水化合物，并使用它们来支持呼吸和生长。为了达到这种碳水化合物的浓度，可以感知并激活适当的代谢途径，并通过必要的过程通过基本过程增加或减少碳的流动。此过程称为代谢反馈。无法调节碳水化合物水平会导致发展缓慢甚至疾病，例如糖尿病。在植物中，这个过程调节了储存的储量的积累，这些储量构成了我们吃的许多食物。植物感觉到可溶性糖的细胞内浓度以及在过多上调的储备沉积时（例如淀粉合成），而在耗尽的时期，从淀粉，脂肪酸或氨基酸中的蛋白质上调了储量分解。在早期的幼苗开发过程中，植物取决于其能源的储存脂肪酸的分解，因为它们尚未开发出通过光合作用从大气中固定碳的设备。提供糖的脂肪酸分解代谢可以抑制糖，从而使这是研究植物中代谢反馈网络的绝佳模型系统。我们最近表明，ABI4基因抑制了模型植物拟南芥中的脂质分解。 ABI4编码转录因子，这意味着它的功能是控制其他基因的活性。 ABI4的许多已知靶基因受代谢状态受到调节，这表明在代谢反馈控制中起作用。 ABI4还起作用对植物激素ABA的反应，众所周知，它在植物糖反应中发挥作用。这项工作旨在了解代谢反馈控制如何在植物中起作用，如何调节ABI4作用以及ABI4如何将脂肪酸分解代谢响应于糖水平。

项目成果

Regulation of Arabidopsis thaliana seed dormancy and germination by 12-oxo-phytodienoic acid.

• DOI: 10.1093/jxb/erw028
• 发表时间: 2016-04
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Dave A;Vaistij FE;Gilday AD;Penfield SD;Graham IA
• 通讯作者: Graham IA