Defining the role of the ABI4 transcription factor in the sugar regulated control of storage oil breakdown in Arabidopsis

定义 ABI4 转录因子在拟南芥贮藏油分解糖调节控制中的作用

• 批准号: BB/E022081/1
• 负责人: Ian Graham
• 金额: $ 56.23万
• 依托单位: University of York
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2008
• 资助国家: 英国
• 起止时间: 2008 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE022081%2F1
• 关键词: Defining; role; ABI4; transcription; factor

All living organisms maintain constant levels of soluble carbohydrates in their cells, and use these to support respiration and growth. In order to achieve this carbohydrate concentration is sensed and appropriate metabolic pathways are activated and repressed to increase or decrease the flow of carbon through essential processes as required. This process is known as metabolic feedback. Failure to regulate carbohydrate levels can lead to slow development or even disease, such as in the case of diabetes. In plants this process regulates the accumulation of stored reserves, which form much of the food we eat. Plants sense the intracellular concentration of soluble sugars and in times of excess up-regulate reserve deposition, such as starch synthesis, and in times of depletion up-regulate reserve breakdown, either from starch, fatty acids or amino acids from proteins. During early seedling development plants are dependent on the breakdown of stored fatty acids for their energy source, as they have not yet developed the apparatus for fixing carbon from the atmosphere through photosynthesis. Fatty acid catabolism can be inhibited by the provision of sugars, making this an excellent model system to study metabolic feedback networks in plants. We have recently shown that the ABI4 gene represses lipid breakdown in the model plant Arabidopsis. ABI4 encodes a transcription factor, meaning that it's function is to control the activity of other genes. Many of the known target genes of ABI4 are subject to regulation by metabolic status, suggesting a role in metabolic feedback control. ABI4 also functions in the response to the plant hormone ABA which is well known to play a role in plant sugar responses. This work aims to understand how metabolic feedback control works in plants, how ABI4 action is regulated and how ABI4 can turn off fatty acid catabolism in response to sugar levels.

所有生物体的细胞中都保持恒定水平的可溶性碳水化合物，并利用它们来支持呼吸和生长。为了实现这个碳水化合物浓度，适当的代谢途径被激活和抑制，以根据需要增加或减少通过基本过程的碳流量。这个过程称为代谢反馈。未能调节碳水化合物水平可能会导致发育缓慢甚至疾病，例如糖尿病。在植物中，这个过程调节储存储备的积累，这些储备构成了我们所吃的大部分食物。植物感知细胞内可溶性糖的浓度，并在过量时上调储备沉积（例如淀粉合成），并在耗尽时上调储备分解（来自淀粉、脂肪酸或蛋白质中的氨基酸）。在幼苗发育早期，植物依赖于储存脂肪酸的分解作为能量来源，因为它们尚未开发出通过光合作用固定大气中碳的装置。提供糖可以抑制脂肪酸分解代谢，这使其成为研究植物代谢反馈网络的绝佳模型系统。我们最近发现 ABI4 基因可抑制模型植物拟南芥中的脂质分解。 ABI4 编码转录因子，这意味着它的功能是控制其他基因的活性。 ABI4 的许多已知靶基因都受到代谢状态的调节，表明其在代谢反馈控制中的作用。 ABI4 还在对植物激素 ABA 的反应中发挥作用，众所周知，ABA 在植物糖反应中发挥作用。这项工作旨在了解植物中的代谢反馈控制如何发挥作用、ABI4 的作用如何受到调节以及 ABI4 如何响应糖水平而关闭脂肪酸分解代谢。

项目成果

Regulation of Arabidopsis thaliana seed dormancy and germination by 12-oxo-phytodienoic acid.

• DOI: 10.1093/jxb/erw028
• 发表时间: 2016-04
• 期刊: Journal of experimental botany
• 影响因子: 6.9
• 作者: Dave A;Vaistij FE;Gilday AD;Penfield SD;Graham IA
• 通讯作者: Graham IA