DEVELOPMENT OF MONKEYS PRODUCED BY EMBRYO SPLITTING & SPERM INJECTION

胚胎分裂产生的猴子的发育

• 批准号: 7349356
• 负责人: GENE P. SACKETT
• 金额: $ 13.2万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349356
• 关键词: DEVELOPMENT; MONKEYS; PRODUCED; EMBRYO; SPLITTING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Progress in experimentally studying the functions of genes discovered now or in the future depends primarily on having appropriate animal models. Similarly, learning how to manipulate genes to alter anatomy, physiology, or behavior, and determining the safety of these manipulations in primates, including humans, depends on appropriate animal models. An important approach to these issues concerns controlling and manipulating the genes of identical twins, who at conception have an identical genome. Recently, a procedure has been developed in which the 4-8 cell embryo of primates (morula stage) is split into individual cells. These cells are implanted into surrogate mothers, who carry a single fetus to term. The surrogate mother may or may not be the actual biological mother. Given appropriate experimental techniques, these identical twin fetuses can serve as control and experimental subjects in studies manipulating almost any aspect of the genome, the mother, the fetal environment, the subject's anatomy and physiology, and/or the postnatal environment. The utility of these potential models using embryo split twins depends largely on exactly how similar the twins are in their anatomy, growth, physiology, and behavior. The two major purposes of this research are (1) to determine the degree of twin similarity in macaque monkey subjects across a wide range of growth, physiological, and behavioral characteristics, and (2) determine whether the embryo splitting and surrogate mothering procedure produces any abnormalities in these characteristics.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。实验研究现在或将来发现的基因功能的进展主要取决于具有适当的动物模型。同样，学习如何操纵基因改变解剖学，生理或行为，并确定包括人类在内的灵长类动物中这些操纵的安全性取决于适当的动物模型。解决这些问题的一种重要方法涉及控制和操纵同卵双胞胎基因的基因，后者在受孕方面具有相同的基因组。最近，已经开发了一种程序，其中4-8个灵长类动物的细胞胚胎（莫拉阶段）分为单个细胞。这些细胞被植入替代母亲，这些母亲携带一个胎儿。代理母亲可能是实际的亲生母亲。鉴于适当的实验技术，这些相同的双胞胎胎儿可以作为控制基因组，母亲，胎儿环境，受试者的解剖学和生理学和/或产后环境几乎任何方面的研究中的对照和实验对象。这些潜在模型使用胚胎分裂双胞胎的实用性在很大程度上取决于双胞胎在解剖学，生长，生理和行为中的相似程度。这项研究的两个主要目的是（1）确定在广泛的生长，生理和行为特征的猕猴猴子受试者中的双胞胎相似性程度，并且（2）确定胚胎分裂和替代母亲程序是否会产生任何这些特征异常。