Microfluidic Refractometric Biosensor

微流控折光生物传感器

基本信息

批准号：
7257825
负责人：
GREGORY ZELTSER
金额：
$ 32.09万
依托单位：
PHYSICAL OPTICS CORPORATION
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-07-15 至 2009-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7257825
关键词：
Adsorption Allergens Anthrax disease Antibodies Area Arts Bacillus anthracis Back Binding Biochemical Biological Biological Assay Biological Markers Biosensor Bioterrorism Blood Blood Circulation Blood capillaries Blood specimen Body Fluids Bontoxilysin Botulinum Toxins Bovine Serum Albumin Buffers Categories Centers for Disease Control and Prevention (U.S.)Characteristics Chemical Warfare Agents Communicable Diseases Complex Computer software Concentration measurement Condition Coupled Data Detection Development Device Designs Devices Diagnosis Disease Doctor of Medicine Doctor of Philosophy Dose Early Diagnosis Endopeptidases Enzyme Inhibitor Drugs Enzyme Inhibitors Enzyme-Linked Immunosorbent Assay Enzymes Exhibits Exposure to Family Feeds Fiber Fiber Optics Fluorescence Fluorescence Resonance Energy Transfer Gelatinase A Goals Gold Health Hospitals Human Human body Hydrolysis Immune System Diseases Individual Injection of therapeutic agent Institution Interstitial Collagenase Label Laboratories Lasers Length Light Magnetism Malignant Neoplasms Marketing Matrilysin Matrix Metalloproteinases Measures Metalloproteases Methods Microbiology Microfluidic Analytical Techniques Microfluidics Microprocessor Microspheres Modeling Modification Monitor Monkeys Morphologic artifacts NIH Program Announcements National Institute of Allergy and Infectious Disease Natural regeneration Noise Operative Surgical Procedures Optics Paramedical Personnel Peptide Hydrolases Peptides Performance Persons Pharmaceutical Preparations Phase Polymers Polystyrenes Population Positioning Attribute Preparation Principal Investigator Process Production Protease Inhibitor Proteins Proteomics Pump Qualifying Radioisotopes Range Reading Recovery Refractive Indices Relaxation Reproducibility Research Resistance Running Saliva Sampling Scientist Screening procedure Signal Transduction Small Business Funding Mechanisms Small Business Innovation Research Grant Solutions Specificity Speed Standards of Weights and Measures Stretching Stromelysin 1 Surface System Techniques Technology Temperature Testing Text Time Tissues Toxin Trifluoroacetic Acid United States National Institutes of Health Urine Variant absorption anthrax lethal factor aqueous assay development base capillary concept cost design emergency service responder experience feeding improved indexing inhibitor/antagonist innovation member millimeter nanoGold nanoparticle next generation novel optical communication optical sensor optical switch pathogen portability prevent protein aminoacid sequence prototype receptor binding response scale up sensor tool transmission process wasting

项目摘要

DESCRIPTION (provided by applicant): The NIAID is seeking to develop assays for the improved early diagnosis of infectious diseases. In response, Physical Optics Corporation (POC) proposes to develop a novel Microfluidic Refractometric Biosensor (MRB) system based on fiber long-period Bragg grating (LPBG) optical probes, the tips of which are coated with a nanolayer gold mirror and positioned in a consecutively connected capillary array of a microfluidic chip. The MRB system will build upon POC's previously developed microfluidic refractometric proteomic array which has demonstrated high sensitivity to metalloproteinase in cancer related research. MRB system consists of three subsystems: (1) detection, (2) processing/analysis, and (3) microfluidic. The detection subsystem includes two pairs of the long period Bragg grating (LPBG) analyzing and reference probes coated with inhibitors of the Bacillus anthracis lethal factor or Botulinum neurotoxin; the processing/analysis subsystem consists of a laser diode, fiber optic cables, connectors, an optical switch, a coupler/beam splitter, and a high-precision optical spectrum analyzer feeding a microprocessor; the microfluidic subsystem combines a microfluidic chip with four channels/capillaries, injection and waste ports, a pump, and tubing with solenoid valves. The Phase I device prototype will perform label-free detection and concentration measurements of two metalloproteinases, anthrax lethal factor and botulinum neurotoxin, in unpurified samples of body fluids. In Phase I POC will design and fabricate an MRB model and demonstrate its sensitivity and selectivity. In Phase II the MRB will be optimized for diagnosis of infectious, allergen-caused, and immunologic diseases, culminating in the fabrication of a fully automatic, compact, durable, and highly sensitive device that will operate without labels. The proposed system will serve as a high-throughput detection platform for rapid recognition of infectious diseases based on protein-signature activity profiles. The system will accurately analyze multiple body fluid samples. Rapid functional proteomic analysis will facilitate early diagnosis, improve treatment, and speed the development of new drugs, all of which will contribute to public and individual health.

描述（由申请人提供）：NIAID正在寻求开发用于改善感染性疾病的早期诊断的测定法。作为响应，物理光学公司（POC）提议开发一种基于纤维长期bragg光栅（LPBG）光学探针的新型微流体折射率生物传感器（MRB）系统微流体芯片的连续连接的毛细管阵列。 MRB系统将基于POC先前开发的微流体折射率蛋白质组学阵列，该阵列在癌症相关研究中对金属蛋白酶的敏感性很高。 MRB系统由三个子系统组成：（1）检测，（2）处理/分析和（3）微流体。检测子系统包括两对长时间Bragg光栅（LPBG）分析和参考探针，并涂有蒽的致命因子或肉毒杆菌神经毒素的抑制剂；处理/分析子系统由激光二极管，光纤电缆，连接器，光学开关，耦合器/束分配器以及高精度光谱分析仪为微处理器供给器；微流体子系统将微流体芯片与四个通道/毛细血管，注入和废物端口，泵和电磁阀的管结合在一起。 I期设备原型将在未纯化的体液样品中对两种金属蛋白酶，致命因子和肉毒杆菌神经毒素进行无标记的检测和浓度测量。在I期中，POC将设计和制造MRB模型，并证明其灵敏度和选择性。在第二阶段，将对MRB进行优化，以诊断感染性，过敏原引起的和免疫疾病，最终在制造全自动，紧凑，耐用和高度敏感的设备的制造中，该设备将在没有标签的情况下运行。提出的系统将作为基于蛋白质签名活动曲线的传染病快速识别的高通量检测平台。该系统将准确分析多种体液样品。快速的功能性蛋白质组学分析将有助于早期诊断，改善治疗并加快新药的发展，所有这些都将有助于公共和个人健康。