Microfluidic Refractometric Biosensor

微流控折光生物传感器

• 批准号: 7160628
• 负责人: GREGORY ZELTSER
• 金额: $ 32.09万
• 依托单位: PHYSICAL OPTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7160628
• 关键词: Bacillus anthracis; anthrax toxin; antibody; body fluids; capillary; endopeptidases; gold; lighting; microcapsule; microfluidics; neurotoxins; polystyrenes; proteomics; toxin

DESCRIPTION (provided by applicant): The NIAID is seeking to develop assays for the improved early diagnosis of infectious diseases. In response, Physical Optics Corporation (POC) proposes to develop a novel Microfluidic Refractometric Biosensor (MRB) system based on fiber long-period Bragg grating (LPBG) optical probes, the tips of which are coated with a nanolayer gold mirror and positioned in a consecutively connected capillary array of a microfluidic chip. The MRB system will build upon POC's previously developed microfluidic refractometric proteomic array which has demonstrated high sensitivity to metalloproteinase in cancer related research. MRB system consists of 3 subsystems: (1) detection, (2) processing/analysis, and (3) microfluidic. The detection subsystem includes 2 pairs of the long period Bragg grating (LPBG) analyzing and reference probes coated with inhibitors of the Bacillus anthracis lethal factor or Botulinum neurotoxin; the processing/analysis subsystem consists of a laser diode, fiber optic cables, connectors, an optical switch, a coupler/beam splitter, and a high-precision optical spectrum analyzer feeding a microprocessor; the microfluidic subsystem combines a microfluidic chip with 4 channels/capillaries, injection and waste ports, a pump, and tubing with solenoid valves. The Phase I device prototype will perform label-free detection and concentration measurements of 2 metalloproteinases, anthrax lethal factor and botulinum neurotoxin, in unpurified samples of body fluids. In Phase I, POC will design and fabricate an MRB model and demonstrate its sensitivity and selectivity. In Phase II, the MRB will be optimized for diagnosis of infectious, allergen-caused, and immunologic diseases, culminating in the fabrication of a fully automatic, compact, durable, and highly sensitive device that will operate without labels. The proposed system will serve as a high-throughput detection platform for rapid recognition of infectious diseases based on protein-signature activity profiles. The system will accurately analyze multiple body fluid samples. Rapid functional proteomic analysis will facilitate early diagnosis, improve treatment, and speed the development of new drugs, all of which will contribute to public and individual health.

描述（由申请人提供）：NIAID 正在寻求开发用于改进传染病早期诊断的检测方法。为此，物理光学公司 (POC) 提议开发一种基于光纤长周期布拉格光栅 (LPBG) 光学探针的新型微流控折射生物传感器 (MRB) 系统，其尖端涂有纳米层金镜并位于连续连接的微流控芯片的毛细管阵列。 MRB 系统将建立在 POC 先前开发的微流体折射蛋白质组阵列的基础上，该阵列已在癌症相关研究中表现出对金属蛋白酶的高敏感性。 MRB 系统由 3 个子系统组成：(1) 检测、(2) 处理/分析和 (3) 微流体。检测子系统包括2对长周期布拉格光栅（LPBG）分析和参考探针，涂有炭疽杆菌致死因子或肉毒杆菌神经毒素的抑制剂；处理/分析子系统由激光二极管、光纤电缆、连接器、光开关、耦合器/分束器和为微处理器供电的高精度光谱分析仪组成；微流控子系统将微流控芯片与 4 个通道/毛细管、注射和废物端口、泵以及带电磁阀的管道结合在一起。第一阶段设备原型将对未纯化的体液样本中的 2 种金属蛋白酶、炭疽致死因子和肉毒杆菌神经毒素进行无标记检测和浓度测量。在第一阶段，POC将设计和制作MRB模型并展示其灵敏度和选择性。在第二阶段，MRB 将针对传染性、过敏原引起的和免疫性疾病的诊断进行优化，最终制造出一种全自动、紧凑、耐用且高度灵敏的设备，无需标签即可运行。所提出的系统将作为一个高通量检测平台，用于根据蛋白质特征活性谱快速识别传染病。该系统将准确分析多个体液样本。快速功能蛋白质组分析将促进早期诊断、改善治疗并加速新药的开发，所有这些都将有助于公众和个人健康。