Microchannel Proteomic Array

微通道蛋白质组阵列

• 批准号: 6643112
• 负责人: GREGORY ZELTSER
• 金额: $ 10万
• 依托单位: PHYSICAL OPTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2003-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6643112
• 关键词: biomedical automation; biotechnology; charge coupled device camera; high throughput technology; intermolecular interaction; ligands; peptide library; protein protein interaction; protein quantitation /detection; protein structure function; proteomics; technology /technique development

DESCRIPTION (provided by applicant): Physical Optics Corporation (POC) proposes to design, develop, fabricate, and test a Microchannel Proteomic Array (MPA) system based on proprietary silicon chip fabrication technology and integration of immunodetection amplified by T7 RNA polymerase (IDAT) and fluorescence-in-situ-hybridization (FISH) techniques. The system will provide high throughput, precise isolation, and quantitative analysis of proteins in unpurified samples of body fluids and/or cancer cells, as well as allow carrying out protein-protein and protein-ligand interaction studies. The MPA system will consist of three components: a microarray chip, a POC backlit holographic diffuser screen, and a processing/analyzing subsystem. The chip will have up to 10,000 micro-channels on a 2. 25 cm2 plate surface and the potential to simultaneously assay ten thousand proteins/ oligopepetides in an unpurified sample in hours. The highly sensitive chip combined with a massively parallel, automated analysis array can yield ultrahigh throughput. The microchip will consume only nanoliters of the sample. In Phase I, POC will investigate micro-channel proteomic array system design and architecture to demonstrate proof-of-concept.

描述（由申请人提供）：物理光学公司（POC）提议设计，开发，制造和测试基于专有硅芯片制造技术的微通道蛋白质组学阵列（MPA）系统，并通过T7 RNA聚合酶（IDAT）扩增免疫探测器的整合和荧光 - 原位杂交（FISH）技术。该系统将在体液和/或癌细胞的未纯净样品中对蛋白质进行高吞吐量，精确的分离和定量分析，并允许进行蛋白质 - 蛋白质蛋白和蛋白质配体相互作用研究。 MPA系统将由三个组件组成：微阵列芯片，POC背光全息扩散器屏幕和处理/分析子系统。该芯片在2. 25 cm2板表面上最多将具有多达10,000个微通道，并在未覆盖的样品中在几个小时内同时测定一千个蛋白质/寡素的潜力。高度敏感的芯片与大规模平行的自动分析阵列结合在一起，可以产生超高的吞吐量。微芯片将仅消耗样品的纳米含量。在第一阶段，POC将研究微通道蛋白质组学阵列系统设计和体系结构，以证明概念验证。