Laser Lancing Device

激光采血装置

• 批准号: 7086305
• 负责人: GREGORY ZELTSER
• 金额: $ 48.6万
• 依托单位: PHYSICAL OPTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7086305
• 关键词: animal care; animal welfare research; bioengineering /biomedical engineering; biomedical equipment development; blood tests; catecholamines; catheterization; diagnosis design /evaluation; diagnosis procedure safety; heat; injury /disease stressor; jugular veins; laboratory mouse; laboratory rat; lasers; pain threshold; phlebotomy; portable biomedical equipment; rapid diagnosis; veterinary science

DESCRIPTION (provided by applicant): The National Institute on Aging is seeking minimally perturbing techniques for collecting blood from mice, rats, and other laboratory animals several times a day in sufficient quantities to measure hormone levels and other circulating factors in young and old animals over the course of long-term experiments. Current blood collection techniques cause pain and stress, and require anesthesia, or are unsuitable for long-terms repeated experiments. No instrument is available for undisturbed and painless blood collection from unanesthetized and unrestrained laboratory rodents 3-4 times a day over long-term experiments. To address this need, Physical Optics Corporation proposes to design, develop, and fabricate a new portable Laser Lancing Device (LLD) for painless and rapid (1-1.5 min.) collection of serial blood samples from the tails of unanesthetized rodents. The main goal of this Phase II project is to develop a complete engineering LLD prototype ready for testing and validation in research laboratories and veterinary clinics. The new LLD blood collecting system will be a technological breakthrough in laboratory animal science, and especially in animal studies that require minimally perturbing blood sampling. Because of the unique properties of the LLD and its improved treatment of laboratory animals, it will also have wide application in animal clinics, animal shelters, and veterinary medicine. This Phase II effort to develop an LLD will include the following: optimizing the laser diode irradiation regime based on an improved optical projection system that will allow shorter pulses and pulse trains, to diminish collateral thermal damage; designing and fabricating inflatable heating cuffs that will be easy to operate and minimally perturbing to the animals; and integrating and packaging the complete engineering LLD prototype for testing and validation in research laboratories and veterinary clinics. When fully developed, the LLD will be a rapid, painless, stress-free, easy-to-use device for collecting serial blood samples from the tails of unanesthetized mice and rats.

描述（由申请人提供）： 美国国家衰老研究所正在寻求最小的扰动技术，以每天几次从小鼠，大鼠和其他实验室动物收集血液，以测量长期实验的年轻和老动物中的激素水平和其他循环因素。当前的血液收集技术会导致疼痛和压力，需要麻醉，或者不适合长期重复实验。在长期实验中，每天3-4次，没有任何仪器可用于不受干扰且无痛的血液收集血液。为了满足这一需求，物理光学公司建议设计，开发和制造一种新的便携式激光衬里装置（LLD），以无痛和快速（1-1.5分钟）收集来自未消除啮齿动物的尾巴的连续血样。该第二阶段项目的主要目标是开发一个完整的工程原型，准备在研究实验室和兽医诊所进行测试和验证。新的LLD血液收集系统将是实验室动物科学的技术突破，尤其是在需要最少扰动血液采样的动物研究中。由于LLD的独特特性及其对实验动物的改进治疗，它也将在动物诊所，动物收容所和兽医医学中广泛应用。开发LLD的II期努力将包括以下内容：基于改进的光投影系统优化激光二极管辐射制度，该系统将允许较短的脉冲和脉冲序列，从而减少侧支热损伤；设计和制造充气加热袖口，这些袖口将易于操作，并且对动物的扰动极少；以及整合和包装完整的工程原型，用于研究实验室和兽医诊所的测试和验证。 当充分发达时，LLD将是一种快速，无痛，无压力，易于使用的设备，用于从一小鼠和大鼠的尾部收集连环血样。