Immunofluorometric Drug Detection System

免疫荧光药物检测系统

• 批准号: 6444435
• 负责人: GREGORY ZELTSER
• 金额: $ 10万
• 依托单位: PHYSICAL OPTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2002-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444435
• 关键词: biomedical equipment development; cannabinoids; cocaine; drug detection; enzyme linked immunosorbent assay; fluorimetry; immunofluorescence technique; portable biomedical equipment; saliva

DESCRIPTION (provided by applicant): Administration of drug testing in the field requires a non-invasive drug assay that can be performed immediately at the site of specimen collection. To address this need, POC proposes to design, develop, and fabricate a unique saliva-based Immunofluorometric Drug Detection System (IDDS) as a rapid, sensitive, and simple-to-use multi-analyte diagnostic tool with disposable cartridges. The IDDS consists of two elements: a high surface area cartridge and a fluorometric reader. The three-dimensional polymer cartridge surface relief is covered with a patchwork of positively and negatively charged binding sites to provide high immobilization density of catching antibodies without steric inhibition. Additionally, this cartridge significantly improves the sensitivity and robustness of the two-step quantitative "sandwich" immunofluorometric assay of drugs in saliva performed in a portable fluorometric reader. The IDDS device will be build on a base of commercially available parts and will include a holographic cartridge. Phase I will culminate in a full proof-of-concept demonstration using an in vitro artificial model. PROPOSED COMMERCIAL APPLICATION: In addition to saliva-based drug detection, the IDDS device also can be used for detection of pathogens in body fluids; rapid immunoassay for hormones, medications, and metabolites; and military surveillance and and monitoring of biological and chemical warfare agents.

描述（由申请人提供）：在 场需要一个非侵入性药物测定，可以立即执行 标本收集的地点。为了满足这一需求，POC建议设计， 开发并制造出独特的基于唾液的免疫荧光计量药物检测 系统（IDD）作为快速，敏感且易于使用的多肛门诊断 具有一次性墨盒的工具。 IDD由两个元素组成：高 表面积墨盒和荧光读取器。三维聚合物 墨盒表面的浮雕被积极的拼布覆盖 带负电荷的结合位点可提供高固定密度 捕获没有空间抑制的抗体。此外，这把墨盒 显着提高了两步的灵敏度和鲁棒性 对唾液中药物进行的定量“三明治”免疫荧光测定法 在便携式荧光读取器中。 IDD设备将建立在 市售的零件，将包括全息墨盒。第一阶段 将使用体外的完整概念示范演示中达到高潮 人工模型。 拟议的商业应用： 除了基于唾液的药物检测外，IDDS设备还可用于检测体液中的病原体。激素，药物和代谢产物的快速免疫测定；以及军事监视以及对生物和化学战代理商的监测。