Ariol SL-50 Workstation and Review Stations

Ariol SL-50 工作站和检查站

• 批准号: 6877240
• 负责人: MARY Kay WASHINGTON
• 金额: $ 33.79万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877240
• 关键词: biomedical automation; biomedical equipment purchase; biomedical resource; clinical chemistry; computer data analysis; computer network; computer program /software; cytodiagnosis; diagnosis quality /standard; exfoliative cytology; fluorescent dye /probe; fluorescent in situ hybridization; immunocytochemistry; molecular /cellular imaging; biomedical automation; biomedical equipment purchase; biomedical resource; clinical chemistry; computer data analysis; computer network; computer program /software; cytodiagnosis; diagnosis quality /standard; exfoliative cytology; fluorescent dye /probe; fluorescent in situ hybridization; immunocytochemistry; molecular /cellular imaging

Investigation of biomarkers of pre-neoplasia as well as markers for prognosis and response to individualized therapies is increasingly important in translational research, a major focus of Vanderbilt University School of Medicine. One recent advance relevant to these translational efforts is the development of tissue microarrays (TMAs), which allow high through-put analysis of expression of numerous markers as determined by immunohistochemistry and immunofluorescence. However, the utility of TMAs is limited by the subjective semi-quantitative nature of the data analysis by individual pathologists. We are requesting funds to purchase an Ariol SL-50 Automated Cellular Scanning Quantitation System to overcome these limitations. This system will obtain digitized images of stained slides at various magnifications, including different types of stains, counterstains, immunohistochemical chromagens, and multiple fluorescent probes. Because the Ariol SL-50 system is automated and can scan in 50 slides overnight, facilitation of analysis of multiple slides without human intervention is possible. The images are easily reviewable by a consulting pathologist to confmn the veracity of interpretation. The most important characteristic of the system is the ability to quantify staining using varying calculations including the area of staining as a percent of total tissue area, staining per cell and number of stained cells and to differentiate patterns of intracellular staining among nuclear, cytoplasmic and plasma membrane locations. Operation of the instrument will be supervised by Dr. Washington, who will also oversee maintenance. Service contract fees will be offset by charging a small user fee per slide. Major users are primarily investigators from the VUMC Lung, GI, and Breast SPOREs, the Skin Diseases Research Center, and the Digestive Diseases Research Center. Use of the system and prioritization of access will be under the oversight of the Utilization Committee. The presence of this instrument will allow us to embark on the large-scale translational biomarker studies that are required for evaluation of diagnostic and prognostic markers. An additional benefit is the ease of data sharing among groups, facilitating interactions among investigators and promoting inter-SPORE collaborations.

调查北极亚前生物标志物以及预后和反应的标志 个性化疗法在翻译研究中越来越重要，这是范德比尔特大学医学院的主要重点。与这些翻译工作相关的最新进展是组织微阵列（TMA）的发展，该阶层允许对通过免疫组织化学和免疫荧光确定的众多标记的表达进行高度贯穿分析。但是，TMA的效用受个人病理学家数据分析的主观半定量性质的限制。我们要求资金购买ARIOL SL-50自动细胞扫描定量系统，以克服这些限制。该系统将在各种大型上获得染色幻灯片的数字化图像，包括 不同类型的污渍，抗风化，免疫组织化学染色体和多种荧光探针。由于Ariol SL-50系统是自动化的，并且可以在50张幻灯片中扫描，因此可以促进对无人干预的多个幻灯片进行分析。咨询病理学家可以很容易地审查这些图像，以了解解释的真实性。该系统的最重要特征是使用各种计算量化染色的能力，包括染色面积为总组织面积的百分比，每个细胞染色和染色细胞的染色，以及在核，细胞质和质膜膜位置之间区分细胞内染色的模式。该工具的运作将由华盛顿博士监督，他还将监督维护。服务合同费用将通过每张幻灯片收取少量用户费用来抵消。主要用户主要是来自VUMC肺，GI和乳腺孢子，皮肤病研究中心和消化疾病研究中心的研究人员。使用系统的使用和访问的优先级将在利用委员会的监督下。该仪器的存在将使我们能够开始评估诊断和预后标记所需的大规模翻译生物标志物研究。另一个好处是群体之间的数据共享易用性，从而促进了研究人员之间的互动并促进了跨越间的合作。