RADIOLOGICAL RESEARCH ACCELERATOR FACILITY (RARAF)

放射学研究加速器设施 (RARAF)

• 批准号: 7118253
• 负责人: DAVID JONATHAN BRENNER
• 金额: $ 88.42万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7118253
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical automation; biomedical equipment; biomedical equipment purchase; biomedical resource; electromagnetic radiation; particle accelerators; radiation detector; radiography; single cell analysis; technology /technique development

DESCRIPTION (provided by applicant): The technology development component focuses on four areas which our users have identified as ripe for enhancement: improved accuracy, enhanced imaging capabilities, extension of the charged-particle microbeam to X rays; and development of a simple stand-alone microbeam that can be used without physics support and can be potentially exported to other labs. A highlight regarding improved accuracy and reliability is that we have just received a $2M NIH high-end equipment grant (plus institutional support) for a new particle accelerator, central to the microbeam facility, which will be installed under the auspices of this P-41 grant. Highlights regarding enhanced imaging and sensor technology will be an on-line custom multi-photon imaging system to allow time-lapse fluorescent imaging of early (scale of seconds to minutes) radiation events in individual live cells, a new imaging system based on quantitative phase microscopy to image live cells and cell nuclei without use of potentially cytotoxic stains, and a system for single-cell microsensors to monitor the flux of molecules such as oxygen and NO, in microbeam-irradiated individual cells. In collaboration with a variety of investigators from outside and within Columbia University, 20 collaborative and service-based research projects are described, which both drive and utilize the new resources. All are logical outgrowths of current federally funded research In addition to the ongoing training of undergraduates, graduate students, and post docs, training will be enhanced through formal programs for high school students, high school teachers, and undergraduates. In addition to dissemination of material through talks, our website, and peer-reviewed publications, we will run a 2nd International Workshop "Imaging and Probing Individual Cells: Application to Signaling, Structure and Function", as well as the biannual "International Workshop on Microbeam Probes of Cellular Radiation Response".

描述（由申请人提供）：技术开发部分重点关注我们的用户认为可以增强的四个领域：提高精度、增强成像能力、将带电粒子微束扩展到 X 射线；以及开发一种简单的独立微束，该微束可以在没有物理支持的情况下使用，并且可以导出到其他实验室。 关于提高准确性和可靠性的一个亮点是，我们刚刚收到了 200 万美元的 NIH 高端设备拨款（加上机构支持），用于购买一台新的粒子加速器，该加速器是微束设施的核心，将在该 P- 的支持下安装41 补助金。 增强成像和传感器技术的亮点将是在线定制多光子成像系统，可对单个活细胞中的早期（秒到分钟）辐射事件进行延时荧光成像，这是一种基于定量阶段的新型成像系统无需使用潜在细胞毒性染色剂即可对活细胞和细胞核进行成像的显微镜，以及用于监测微束照射的单个细胞中氧气和一氧化氮等分子通量的单细胞微传感器系统。 与哥伦比亚大学外部和内部的各种研究人员合作，描述了 20 个协作和基于服务的研究项目，这些项目既驱动又利用新资源。 所有这些都是当前联邦政府资助的研究的合理产物除了对本科生、研究生和博士后的持续培训之外，还将通过针对高中生、高中教师和本科生的正式计划加强培训。 除了通过讲座、我们的网站和同行评审出版物传播材料外，我们还将举办第二届国际研讨会“成像和探测单个细胞：在信号、结构和功能中的应用”，以及每两年一次的“国际研讨会”细胞辐射响应的微束探针”。