3-D Thermoacoustic Computed Tomography/Molecular Imaging

3-D 热声计算机断层扫描/分子成像

• 批准号: 6992695
• 负责人: ROBERT A KRUGER
• 金额: $ 43.41万
• 依托单位: OPTOSONICS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-01-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6992695
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; computed axial tomography; computer program /software; computer system design /evaluation; cyanine; fluorescent dye /probe; laboratory mouse; phantom model; three dimensional imaging /topography

DESCRIPTION (provided by applicant): We propose to develop a thermoacoustic computed tomography (TCT) scanner for localizing optical-dye-labeled molecular probes in laboratory mice in vivo. This device offers two significant improvements over fluorescence intensity measurements: 1. Fully three-dimensional images will be acquired using a single axial scan of a novel, cylindrical detector army. 2. Exogenous molecular probes, to which a cyanine dye has been attached, will be detected using a dual-wavelength imaging approach. In Phase I we intend to quantify the spatial resolution and low contrast detection limitations of a TCT device that incorporates a 128-element, cylindrical transducer array. These studies will be carried out in tissue-mimicking phantoms, and the results compared to those we have acquired using a TCT device that incorporates a conventional, linear transducer array. We will employ a dual-wavelength, differential-measurement technique for isolating two non-tumor specific carbocyanine dyes (indocyanine dye and cypate), and a tumor-specific optical imaging agent (cytate) with peak absorption at 790 nm. These probes have high extinction coefficient (> 150,000 M-1 cm-1) and low fluorescence quantum yield (<20%), characteristics that favor thermoacoustic imaging. Our goals are to: 1. achieve < 0.5-mm high-contrast, spatial resolution in three dimensions, and 2. image a dye concentration of < 100 nM with a signal-to-noise ratio (SNR) of > 5 within a 1 mm3 volume (100 fmoles) embedded in a tissue-mimicking phantom. In Phase II we will construct a prototype TCT scanner and site the device at the Optical Radiology Laboratory, Washington University, St. Louis, MO. There, researchers will utilize novel monomolecular multimodal imaging agents to compare the imaging capabilities of this TCT scanner to a commercial mu-PET scanner and conventional fluorescence intensity imaging system in a series of phantom and in vivo mouse-imaging experiments.

描述（由申请人提供）： 我们建议开发一个热声计算机断层扫描仪（TCT）扫描仪，用于在体内实验室小鼠中定位光染色标记的分子探针。该设备比荧光强度测量值进行了两个重大改进：1。使用新型的圆柱探测器军队的单个轴向扫描将获得完全三维图像。 2。将使用双波长成像方法检测到氰染料的外源分子探针。在第一阶段，我们打算量化包含128个元素的圆柱传感器阵列的TCT设备的空间分辨率和低对比度检测局限性。这些研究将在模拟组织的幻影中进行，与使用TCT设备合并的TCT设备相比，结果将其结果进行。我们将采用双波长，差异测定技术来隔离两种非肿瘤特异性的碳酸染料染料（吲哚氰氨基染料和Cypate），并在790 nm处隔离具有峰值吸收的肿瘤特异性光学成像剂（细胞）。这些探针具有高灭绝系数（> 150,000 m-1 cm-1）和低荧光量子产率（<20％），这是有利于热声成像的特征。我们的目标是：1。实现<0.5毫米的高对比度，三个维度的空间分辨率和2。图像染料浓度<100 nm，具有信噪比（SNR）> 5的染料浓度在1 mm3体积（100 fmoles）内嵌入了组织模仿谎言中。在第二阶段，我们将构建一个原型TCT扫描仪，并在密苏里州圣路易斯华盛顿大学的光放射学实验室进行设备。在那里，研究人员将利用新型的单分子多模式成像剂将TCT扫描仪的成像能力与一系列幻影和体内小鼠成像实验中的商业MU-PET扫描仪和常规荧光强度成像系统进行比较。