Development and Integration of Bioluminescent CT

生物发光CT的开发和集成

• 批准号: 7053410
• 负责人: Ge Wang
• 金额: $ 25.13万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-14 至 2006-11-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7053410
• 关键词: X ray; bioimaging /biomedical imaging; bioluminescence; biomedical equipment development; computed axial tomography; gene expression; imaging /visualization /scanning; laboratory mouse; lung

DESCRIPTION (provided by applicant): Small animals, particularly genetically engineered mice, are of increasing importance to understand the biologic basis for pathologic disease manifestations. Major efforts are being made to link the genome to the phenotypic expression in both form and function ("Physiome"). Small animal imaging offers the opportunity to evaluate pathologic progression in a much-compressed time frame and with a much-improved resolution. In this R21/R33 proposal, we plan to develop and integrate the first bioluminescent CT (BLCT) device for imaging gene expression and a state-of-the-art micro-CT scanner for studies of the lung in the living mouse model. Our long term goal is to advance molecular and micro imaging technology, and make the proposed integrated system an in vivo tool in biomedical applications, especially for small animal tomography. The specific aims of the R21 Phase are to (1) develop a data acquisition module for bioluminescent imaging of the mouse lung; (2) establish the forward model for bioluminescence based on the optical properties of the mouse as derived from its CT volume; (3) demonstrate the feasibility of bioluminescent tomography in numerical simulation and phantom experiments. The specific aims of the R33 Phase are to (1) prototype a bioluminescent CT device with I0 CCD cameras, and develop an iterative algorithm for a spatial resolution on the order of 1 ram; (2) build a multi-resolution micro-CT scanner with dual imaging, and develop analytic algorithms for high quality reconstructions, including the in vivo mode with about 20 micron spatial resolution and 20 second temporal resolution and a maximal spatial resolution on the order of 5 microns; (3) integrate the two cutting-edge CT modalities (optical and X-ray), and demonstrate the feasibility and utility of the integrated system in small animal studies. Upon completion of the R21/33 project, a unique micro-tomography system will have been developed with molecular, multi-resolution, multi-energy, dynamic and bioluminescent imaging capabilities. We will demonstrate, through example projects, the utility of the system in generating critical physiological and pathological information of the lung.

描述（由申请人提供）： 小型动物，尤其是基因工程小鼠，对了解病理疾病表现的生物学基础的重要性越来越重要。正在做出的重大努力将基因组与形式和功能的表型表达联系起来（“ Physiome”）。小型动物成像提供了在备受压制的时间范围内评估病理进展的机会，并通过大量改良的分辨率进行评估。在此R21/R33提案中，我们计划开发和整合第一个用于成像基因表达的生物发光CT（BLCT）设备，以及用于活小鼠模型中肺部研究的最先进的Micro-CT扫描仪。我们的长期目标是推进分子和微型成像技术，并使所提出​​的集成系统成为生物医学应用中的体内工具，尤其是对于小型动物断层扫描。 R21相的具体目的是（1）开发一个数据采集模块，用于小鼠肺的生物发光成像； （2）基于其CT体积得出的小鼠的光学特性建立生物发光的正向模型； （3）证明了生物发光层析成像在数值模拟和幻影实验中的可行性。 R33相的具体目的是（1）具有I0 CCD摄像机的生物发光CT设备的原型，并开发出一种迭代算法，以实现1 RAM的空间分辨率； （2）用双成像构建多分辨率的微CT扫描仪，并为高质量重建的分析算法开发分析算法，包括具有约20微米空间分辨率的体内模式和20秒的时间分辨率和20秒的时间分辨率以及5微米顺序的最大空间分辨率； （3）整合了两种尖端的CT模式（光学和X射线），并证明了综合系统在小动物研究中的可行性和实用性。 R21/33项目完成后，将通过分子，多分辨率，多能，动态和生物发光成像功能开发独特的微型摄影系统。我们将通过示例项目证明该系统在产生肺的关键生理和病理信息方面的实用性。